[1]杨道茂,王奇志,裴真巧.5株植物内生真菌生物转化柠檬烯和月桂烯产物分析[J].华侨大学学报(自然科学版),2023,44(3):374-380.[doi:10.11830/ISSN.1000-5013.202212034]
　YANG Daomao,WANG Qizhi,PEI Zhenqiao.Analysis of Biotransformation Products of Limonene and Myrcene by Five Plant Endophytic Fungi[J].Journal of Huaqiao University(Natural Science),2023,44(3):374-380.[doi:10.11830/ISSN.1000-5013.202212034]
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5株植物内生真菌生物转化柠檬烯和月桂烯产物分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第44卷
期数:
2023年第3期
页码:
374-380
栏目:
出版日期:
2023-05-12

文章信息/Info

Title:
Analysis of Biotransformation Products of Limonene and Myrcene by Five Plant Endophytic Fungi
文章编号:
1000-5013(2023)03-0374-07
作者:
杨道茂;  王奇志;  裴真巧
华侨大学 化工学院, 福建 厦门 361021
Author(s):
YANG Daomao;  WANG Qizhi;  PEI Zhenqiao
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
生物转化;  内生真菌;  柠檬烯;  月桂烯;  柠檬烯-1; 2-二醇
Keywords:
biotransformation;  endophytic fungi;  limonene;  myrcene;  limonene-1; 2-diol
分类号:
Q53
DOI:
10.11830/ISSN.1000-5013.202212034
文献标志码:
A
摘要:
为了筛选具有转化利用单萜类化合物能力的微生物菌株,以5株植物内生真菌为生物催化剂,(+)-柠檬烯、(-)-柠檬烯、月桂烯为生物转化底物,结合气相色谱-质谱联用技术考察生物转化产物.结果表明:5株植物内生真菌具有生物转化(+)-柠檬烯、(-)-柠檬烯、月桂烯的能力;当底物为(+)-柠檬烯时,主要产物均为柠檬烯-1,2-二醇,其中,菌株Diaporthe Nitschke PS10的产量最高,达到4.57 g·L-1;当底物为(-)-柠檬烯时,主要产物为柠檬烯-1,2-二醇,产量为0.81~2.04 g·L-1;当底物为月桂烯时,转化产物有环氧罗勒烯、氧化芳樟醇、柠檬烯-1,2-二醇等化合物;植物内生真菌是一种很有发展潜力的生物催化剂.
Abstract:
In order to screen microbial strains with the ability to transform and utilize monoterpene compounds, five plant endophytic fungi were used as biocatalysts,(+)-limonene,(-)-limonene and myrcene were used as biotransformation substrates, and the biotransformation products were investigated by Gas Chromatography-Mass Spectrometry. The results show that five plant endophytic fungi possess the ability to biotransform(+)-limonene,(-)-limonene and myrcene. When the substrate is(+)-limonene, the main product is limonene-1,2-diol, and the highest yield is 4.57 g·L-1 when the strain is Diaporthe Nitschke PS10. When the substrate is(-)-limonene, the main product is also limonene-1,2-diol, and the yield is 0.81 to 2.04 g·L-1. When the substrate is myrcene, the conversion products include myroxyde, linalool oxide, limonene-1,2-diol and other compounds. It is indicated that plant endophytic fungi are potential biocatalysts.

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备注/Memo

备注/Memo:
收稿日期: 2022-12-28
通信作者: 杨道茂(1975-),男,讲师,博士,主要从事微生物生物转化的研究.E-mail:ydmao@hqu.edu.cn.
基金项目: 国家级大学生科创项目(202110385039); 福建省农业重点自然科学基金资助项目(2014N0026); 福建省泉州市校(院)地协同创新专项(重点)项目(2014Z101); 福建省个人和团队科技员项目(2022年度); 中医药公共卫生服务补助专项(财社［2018］43
更新日期/Last Update: 2023-05-20