[1]裴真巧,王奇志,杨道茂,等.胜红蓟内生真菌转化柠檬烯产物的抑菌活性[J].华侨大学学报(自然科学版),2023,44(1):87-93.[doi:10.11830/ISSN.1000-5013.202207028]
　PEI Zhenqiao,WANG Qizhi,YANG Daomao,et al.Antibacterial Activity of Limonene Product Transformed by Ageratum conyzoides Endophytic Fungi[J].Journal of Huaqiao University(Natural Science),2023,44(1):87-93.[doi:10.11830/ISSN.1000-5013.202207028]
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胜红蓟内生真菌转化柠檬烯产物的抑菌活性()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第44卷
期数:
2023年第1期
页码:
87-93
栏目:
出版日期:
2023-01-10

文章信息/Info

Title:
Antibacterial Activity of Limonene Product Transformed by Ageratum conyzoides Endophytic Fungi
文章编号:
1000-5013(2023)01-0087-07
作者:
裴真巧;  王奇志;  杨道茂;  于海玲
华侨大学 化工学院, 福建 厦门 361021
Author(s):
PEI Zhenqiao;  WANG Qizhi;  YANG Daomao;  YU Hailing
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
胜红蓟;  内生真菌;  柠檬烯;  生物转化;  抑菌活性
Keywords:
Ageratum conyzoides L.;  endophytic fungi;  limonene;  biotransformation;  antibacterial activity
分类号:
O657.6
DOI:
10.11830/ISSN.1000-5013.202207028
文献标志码:
A
摘要:
为考察入侵植物胜红蓟内生真菌转化柠檬烯产物的抑菌活性,以柠檬烯二醇作为标品对照,采用薄层色谱法筛选具有转化柠檬烯能力的胜红蓟内生真菌.通过气相色谱-质谱法对菌株的转化产物进行分析,并对其生物转化柠檬烯发酵液乙酸乙酯粗提物的抑菌活性进行研究.从22株胜红蓟内生真菌中共筛选得到14株具有转化柠檬烯能力的菌株,其中,有6个菌株的柠檬烯转化乙酸乙酯粗提物中含有柠檬烯-1,2-二醇;菌株Diaporthe phaseolorum(wl01)的柠檬烯转化产物中,柠檬烯-1,2-二醇的质量浓度最高(3.43 mg· mL-1);菌株Alternaria alternata(wp06)生物转化柠檬烯发酵液的乙酸乙酯提取物对柑橘意大利青霉类致病菌有一定的抑制效果,对意大利青霉的抑菌效果为(40.03±3.58)%.
Abstract:
In order to explore the antibacterial activity of limonene conversion by endophytic fungi from invasive plant Ageratum conyzoides L., using limonene-1,2-diol as the standard control, the endophytic fungi with the ability to transform limonene were screened by thin layer chromatography. The transformation products of the strain were analyzed by gas chromatography-mass spectrometry, and the antibacterial activity of the ethyl acetate extracts of the biotransformed limonene fermentation broth was studied. It was found that 14 strains with the ability to transform limonene were screened from 22 Ageratum conyzoides L. endophytic fungi, among them, 6 strains of limonene-converted ethyl acetate crude extracts contained limonene-1,2-diol. The mass concentration of limonene-1,2-diol in the limonene conversion product of strain Diaporthe phaseolorum(wl01)was the highest(3.43 mg · mL-1 ). The ethyl acetate extract of strain Alternaria alternata(wp06)biotransformed limonene fermentation broth had a certain inhibitory effect on Penicillium italicum, and the inhibitory effect on Penicillium italicum was(40.03±3.58)%.

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备注/Memo

备注/Memo:
收稿日期: 2022-07-25
通信作者: 王奇志(1976-),女,副教授,博士,主要从事入侵植物资源化利用的研究.E-mail:wqz@hqu.edu.cn.
基金项目: 福建省农业重点自然科学基金资助项目(2014N0026); 福建省泉州市校(院)地协同创新专项(重点)项目(2014Z101); 2018 年中医药公共卫生服务补助专项(财社〔2018〕43)
更新日期/Last Update: 2023-01-20