[1]周丰,连惠婷,曹学功,等.掺杂石墨烯分子印迹传感器对色氨酸的手性识别[J].华侨大学学报(自然科学版),2012,33(5):528-534.[doi:10.11830/ISSN.1000-5013.2012.05.0528]
　ZHOU Feng,LIAN Hui-ting,CAO Xue-Gong,et al.Enantioselective Recognition of Tryptophan Enantiomers by Graphene Doped Molecularly Imprinted Electrochemical Sensor[J].Journal of Huaqiao University(Natural Science),2012,33(5):528-534.[doi:10.11830/ISSN.1000-5013.2012.05.0528]
点击复制

掺杂石墨烯分子印迹传感器对色氨酸的手性识别()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第5期
页码:
528-534
栏目:
出版日期:
2012-09-20

文章信息/Info

Title:
Enantioselective Recognition of Tryptophan Enantiomers by Graphene Doped Molecularly Imprinted Electrochemical Sensor
文章编号:
1000-5013(2012)05-0528-07
作者:
周丰;  连惠婷;  曹学功;  刘斌
华侨大学 材料科学与工程学院, 福建 厦门 361021
Author(s):
ZHOU Feng;  LIAN Hui-ting;  CAO Xue-Gong;  LIU Bin
College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China
关键词:
石墨烯;  分子印迹传感器;  手性识别;  色氨酸
Keywords:
graphene;  molecularly imprinted sensors;  chiral recognitiony;  tryptophan
分类号:
O657.1
DOI:
10.11830/ISSN.1000-5013.2012.05.0528
文献标志码:
A
摘要:
以掺杂了石墨烯纳米片的壳聚糖为功能基体,L-色氨酸为模板分子,利用恒电位沉积法制备对L-色氨酸具有手性识别功能的分子印迹传感器.采用扫描电子显微镜(SEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)等方法表征印迹膜的形成过程.探讨印迹传感器的电化学性能,并优化了最优检测条件.研究结果表明:石墨烯掺杂量为1 mg·mL-1,沉积时间为300 s,工作电压为+0.85 V,溶液pH值为6时,所制备的石墨烯-壳聚糖印迹传感器具有良好的手性识别性能,且对L-色氨酸的浓度线性响应范
Abstract:
A novel electrochemical sensor was prepared by graphene-chitosan molecularly imprinted film, which casted on the gassy carbon electrode by electrodeposition, using L-tryptophan(L-Trp)as the template. The prepared film was investigated by scanning electron microscopy(SEM), fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD). The enantioselective performance and the optimal conditions for L-Trp detection were evaluated by electrochemical technique in detail. Under the optimum conditions of 1 mg·mL-1 graphene doping concentration, 300 s electrodepositing time, the prepared sensor showed a excellent chiral recognition to Trp in pH=6 NaCl solution and linearly selective detection of L-Trp in the range of 0.17~25 μmol·L-1 was found, with a limit of detection of 0.04 μmol·L-1(S/N=3).

参考文献/References:

[1] 郭智成.对美国L-色氨酸事件的回顾和思考[J].医学与哲学,2001,22(8):60-61.
[2] 王大慧,韦萍,欧阳平凯.D-色氨酸研究进展[J].化工进展,2002,21(2):103-105.
[3] 甄乾娜,黄小兰,张晓清,等.高效液相色谱程序波长紫外检测法同时测定血浆中的色氨酸及其代谢物[J].Spzz,2011,29(5):435-438.
[4] Da SILVA M S,VAO E R,TEMTEM M,et al.Clean synthesis of molecular recognition polymeric materials with chiral sensing capability using supercritical fluid technology[J].Biosens Bioelectron,2010,25(7):1742-1747.
[5] 马东杰,许旭,廉伟,等.高效液相色谱法检测胃液中色氨酸和利多卡因[J].分析化学,2009,37(11):1617-1621.
[6] 乔成栋,宋平顺,严祥,等.5种生物胺的毛细管胶束电动色谱分离[J].分析化学,2007,35(1):95-98.
[7] BELIN G K,GARTNER V,SEEGER S.Rapid analysis and sensitive detection of D,L-tryptophan by using shorter capillary column coupled with deep-UV fluorescence detector[J].J Chromatogr B,2009,877(29):3753-3756.
[8] YAN Hang-yan,ROW K H.Enantioseparation condition of D,L-tryptophan using ligand exchange chromatography[J].Indian J Chem A,2008,47(1):75-80.
[9] PRIVETT B J,SHIN J H,SCHOENFISCH M H.Electrochemical sensors[J].Analytical Chemistry,2008,80(12):4499-4517.
[10] CHEN Yang-ping,LIU Bin,LIAN Hui-ting,et al.Preparation and application of urea electrochemical sensor based on chitosan molecularly imprinted films[J].Electroanal,2011,23(6):1454-1461.
[11] 连惠婷,陈娟娟,薛燕,等.久效磷分子印迹传感器的电化学响应特性[J].华侨大学学报:自然科学版,2011,32(2):182-187.
[12] 殷静芬,连惠婷,孙向英,等.尿酸分子印迹电化学传感器的研制及其应用[J].华侨大学学报:自然科学版,2012,33(1):33-38.
[13] VASAPOLLO G,DEL SOLE R,MERGOLA L,et al.Molecularly imprinted polymers:Present and future prospective[J].Int J Mol Sci,2011,12(9):5908-5945.
[14] ZHANG Yuan-bo,TAN Yan-wen,STORMER H L,et al.Experimental observation of the quantum Hall effect and Berry’s phase in graphene[J].Nature,2005,438(7065):201-204.
[15] SHAFRANJUK S E.Electromagnetic properties of the graphene junctions[J].European Physical Journal B-Condensed,2011,80(3):379-393.
[16] MOROZOV S,NOVOSELOV K,KATSNELSON M,et al.Giant intrinsic carrier mobilities in graphene and its bilayer[J].Physical Review Letters,2008,100(1):16602-16604.
[17] GEIM A K.Graphene:Status and Prospects[J].Science,2009,324(5934):1530-1534.
[18] HILL E W,VIJAYARAGAHVAN A,NOVOSELOV K.Graphene Sensors[J].Sensors Journal, IEEE,2011,11(12):3161-3170.
[19] DEMETGUL C,SERIN S.Synthesis and characterization of a new vic-dioxime derivative of chitosan and its transition metal complexes[J].Carbohydrate Polymers,2008,72(3):506-512.
[20] WANG Shao-feng,SHEN Lu,ZHANG Wei-de,et al.Preparation and mechanical properties of chitosan/carbon nanotubes composites[J].Biomacromolecules,2005,6(6):3067-3072.
[21] LI Waoguo,XU Shudong,TANG Min,et al.Direct electrochemistry of horseradish peroxidase on graphene-modified electrode for electrocatalytic reduction towards H2O2[J].Electrochimica Acta,2011,56(3):1144-1149.
[22] NETHRAVATHI C,RAJAMATHI M.Chemically modified graphene sheets produced by the solvothermal reduction of colloidal dispersions of graphite oxide[J].Carbon,2008,46(14):1994-1998.
[23] 蒋挺大.壳聚糖[M].北京:化学工业出版社,2001.
[24] CAO Xiao-ding,DONG Hua,LI Chang-min,et al.The enhanced mechanical properties of a covalently bound chitosan-multiwalled carbon nanotube nanocomposite[J].Journal of Applied Polymer Science,2009,113(1):466-472.
[25] WAN Yi,LIN Zhi-feng,ZHANG Dun,et al.Impedimetric immunosensor doped with reduced graphene sheets fabricated by controllable electrodeposition for the non-labelled detection of bacteria[J].Biosens Bioelectron,2011,26(5):1959-1964.
[26] 农兰平,黄敏,庄玉.L-色氨酸分子印迹壳聚糖膜的制备及透过选择性[J].化学研究,2009,20(3):15-18.

相似文献/References:

[1]方艳红,连慧婷,陈国华.石墨烯/壳聚糖修饰玻碳电极测定水样中痕量铜离子[J].华侨大学学报(自然科学版),2011,32(5):529.[doi:10.11830/ISSN.1000-5013.2011.05.0529]
　FANG Yan-hong,LIAN Hui-ting,CHEN Guo-hua.Determination of Copper with Graphene Nanoplatelets-Chitosan Modified Glassy Carbon Electrode[J].Journal of Huaqiao University(Natural Science),2011,32(5):529.[doi:10.11830/ISSN.1000-5013.2011.05.0529]
[2]孙兆辉,连惠婷,孙向英,等.石墨烯掺杂对分子印迹电化学传感器的增敏作用[J].华侨大学学报(自然科学版),2012,33(4):401.[doi:10.11830/ISSN.1000-5013.2012.04.0401]
　SUN Zhao-hui,LIAN Hui-ting,SUN Xiang-ying,et al.Sensitivity Enhancement of Graphene Doped Molecular Imprinting Electrochemical Sensors[J].Journal of Huaqiao University(Natural Science),2012,33(5):401.[doi:10.11830/ISSN.1000-5013.2012.04.0401]
[3]王国权,庞素秋,林俊生,等.石墨烯及其衍生物在生物医药领域的研究进展[J].华侨大学学报(自然科学版),2018,39(6):787.[doi:10.11830/ISSN.1000-5013.201705036]
　WANG Guoquan,PANG Suqiu,LIN Junsheng,et al.Research Progress of Graphene and Its Derivatives Biomedicine Field[J].Journal of Huaqiao University(Natural Science),2018,39(5):787.[doi:10.11830/ISSN.1000-5013.201705036]

备注/Memo

备注/Memo:
收稿日期: 2012-04-18
通信作者: 刘斌(1963-),男,教授,主要从事电化学传感器的研究.E-mail:bliu@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(20955001,21175049); 福建省自然科学基金计划资助项目(2011J01049); 中央高校基本科研业务费专项基金资助项目(10QZR13)
更新日期/Last Update: 2012-09-20