[1]葛慧华,林锦霞,张光亚.以响应面法优化短小芽孢杆菌木聚糖酶产酶条件[J].华侨大学学报(自然科学版),2012,33(2):172-175.[doi:10.11830/ISSN.1000-5013.2012.02.0172]
　GE Hui-hua,LIN Jin-xia,ZHANG Guang-ya.Optimization of the Fermentation Conditions of Xylanase Production from Bacillus pumilus with Response Surface Analysis[J].Journal of Huaqiao University(Natural Science),2012,33(2):172-175.[doi:10.11830/ISSN.1000-5013.2012.02.0172]
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以响应面法优化短小芽孢杆菌木聚糖酶产酶条件()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第2期
页码:
172-175
栏目:
出版日期:
2012-03-20

文章信息/Info

Title:
Optimization of the Fermentation Conditions of Xylanase Production from Bacillus pumilus with Response Surface Analysis
文章编号:
1000-5013(2012)02-0172-04
作者:
葛慧华; 林锦霞; 张光亚
华侨大学化工学院
Author(s):
GE Hui-hua;  LIN Jin-xia;  ZHANG Guang-ya
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
木聚糖酶;  短小芽孢杆菌;  响应面分析;  交互影响
Keywords:
xylanase;  Bacillus pumilus;  response surface analysis;  interaction
分类号:
TQ925
DOI:
10.11830/ISSN.1000-5013.2012.02.0172
文献标志码:
A
摘要:
应用响应面分析对短小芽孢杆菌木聚糖酶的产酶条件进行优化,得到碳源、氮源及培养时间3种因素交互影响的回归方程.从该方程得到理论最佳产酶条件:培养时间为24h,麸皮、玉米粉、蛋白胨和NH4Cl的质量浓度分别为10,5,5,10g.L-1.在优化条件下,实际最高产酶量为12.1mkat.L-1,与理论最高产酶量12.0mkat.L-1接近,比初始酶活提高12.2倍.
Abstract:
The response surface analysis was applied to optimize the fermentation conditions of xylanase production from Bacillus pumilus.It resulted in a regression equation which reflected the interaction effect among carbon resource,nitrogen resource and cultivation time.The optimized fermentation conditions of xylanase production were: cultivation time 24 h,the proportion of bran and corn power 10∶5,the proportion of peptone and NH4Cl 5∶10.In this optimal conditions,the level of xylanase production was 12.1 mkat·L-1,with an increase of 12.2-fold compared with the enzyme production in the basic medium.The experimental value was very close to the theoretic value,which was 12.0 mkat·L-1 according to the regression equation.

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(2009J01030); 华侨大学科研基金资助项目(07HZR20)
更新日期/Last Update: 2014-03-23