[1]李晨,陈宏文.米曲霉木聚糖酶高产菌株选育[J].华侨大学学报(自然科学版),2011,32(6):663-667.[doi:10.11830/ISSN.1000-5013.2011.06.0663]
　LI Chen,CHEN Hong-wen.Breed of Aspergillus oryzae Producing High-Yield Xylanase[J].Journal of Huaqiao University(Natural Science),2011,32(6):663-667.[doi:10.11830/ISSN.1000-5013.2011.06.0663]
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米曲霉木聚糖酶高产菌株选育()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第32卷
期数:
2011年第6期
页码:
663-667
栏目:
出版日期:
2011-11-20

文章信息/Info

Title:
Breed of Aspergillus oryzae Producing High-Yield Xylanase
文章编号:
1000-5013(2011)06-0663-05
作者:
李晨; 陈宏文
华侨大学工业生物技术福建省高校重点实验室
Author(s):
LI Chen;  CHEN Hong-wen
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
木聚糖酶;  米曲霉;  紫外诱变;  纤维素溶剂木质纤维素分馏法;  半纤维素
Keywords:
xylanase;  Aspergillus oryzae;  ultraviolet irradiation;  cellulose solvent-based lignocellulose fractionation;  hemi·cellulose
分类号:
Q933
DOI:
10.11830/ISSN.1000-5013.2011.06.0663
文献标志码:
A
摘要:
以米曲霉C-2为出发菌株,紫外诱变后,利用透明圈初筛,再分别以农业废弃物1%蔗渣-1%麸皮复合物、2%蔗渣、2%碱提蔗渣半纤维素和2%纤维素溶剂分馏法(CSLF)提取的蔗渣半纤维素为碳源摇瓶复筛,得到一株高产木聚糖酶的米曲霉菌株A73.结果表明:米曲霉A73在上述碳源培养基中,产木聚糖酶酶活力比出发菌株分别提高了281.15%,82.81%,56.09%和33.11%; CSLF蔗渣半纤维素诱导米曲霉C-2及其诱变菌株产酶能力较弱,明显低于其他3种诱导物.
Abstract:
Aspergillus oryzae C-2 was treated with ultraviolet irradiation.High-yield xylanase mutant A73 was obtained after primary transparent circle screening and secondary screening in which 4 different carbon sources-1% wheat bran-1% sugarcane bagasse,2% sugarcane bagasse,2% alkali pretreatment hemi-cellulose and 2% CSLF(cellulose solvent-based lignocellulose fractionation) pretreatment hemi-cellulose were used as fermentation substrates.The xylanase activity of Aspergillus oryzae A73 improved 281.15%,82.81%,56.09% and 33.11% compared with the original strain on respective carbon source,but the xylanase activity induced by CSLF pretreatment hemi-cellulose was apparently lower than that of other substrates.

参考文献/References:

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(D0810015); 教育部留学回国人员科研启动项目(2010); 福建省高等学校新世纪优秀人才支持计划项目(07FJRC03)
更新日期/Last Update: 2014-03-23