[1]王谦,李夏兰,程珊影,等.生物酶降解米糠释放阿魏酸工艺条件优化[J].华侨大学学报(自然科学版),2011,32(1):62-66.[doi:10.11830/ISSN.1000-5013.2011.01.0062]
　WANG Qian,LI Xia-lan,CHENG Shan-ying,et al.Process Optimization of Ferulic Acid Released from Rice Bran by Biological Enzymolysis[J].Journal of Huaqiao University(Natural Science),2011,32(1):62-66.[doi:10.11830/ISSN.1000-5013.2011.01.0062]
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生物酶降解米糠释放阿魏酸工艺条件优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第32卷
期数:
2011年第1期
页码:
62-66
栏目:
出版日期:
2011-01-20

文章信息/Info

Title:
Process Optimization of Ferulic Acid Released from Rice Bran by Biological Enzymolysis
文章编号:
1000-5013(2011)01-0062-05
作者:
王谦; 李夏兰; 程珊影; 方柏山
华侨大学化工学院
Author(s):
WANG Qian;  LI Xia-lan;  CHENG Shan-ying;  FANG Bai-shan
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
阿魏酸;  米糠;  均匀设计;  木聚糖酶;  生物降解
Keywords:
ferulic acid;  rice bran;  uniform design;  xylanase;  biodegradation
分类号:
Q814.9
DOI:
10.11830/ISSN.1000-5013.2011.01.0062
文献标志码:
A
摘要:
采用均匀设计法设计和二次多项式逐步回归分析,优化一株放线菌降解米糠释放反式阿魏酸的培养基配方,并采用单因素法优化发酵条件.优化后培养基配方:米糠质量分数为6%,米糠粒径0.50mm,酵母粉质量分数为1.0%; 优化后的发酵条件:发酵温度为28℃,摇床转速200r.min-1,装液系数为12%,接种量的体积分数为3.3%,pH值8.0.实验结果表明,反式阿魏酸的释放率最高为14.55%,与优化前相比,释放率提高了106.7%.研究阿魏酸酯酶和木聚糖酶联合作用于米糠产阿魏酸和低聚糖的协同效应,阿魏酸释放率达到20.22%,与阿魏酸酯酶单独降解米糠相比,其释放率提高了119.1%; 低聚木糖的释放量2.04mg.mL-1,与木聚糖酶单独降解米糠相比,其释放量提高了87.2%.
Abstract:
The optimal media formulations of ferulic acid released from rice bran by actinomycosis were obtained by uniform-design and quadratic polynomial regression techniques.The best prescription was: rice bran 6%(mass fraction),rice bran granularity 0.50 mm,yeast powder 1.0%(mass fraction).On this basis,the optimal ferment condition was confirmed by single factor method.The optimal conditions were as follows: fermentation temperature 28 ℃,rotation speed 200 r·min-1,liquid volume in flask 12%,inoculation quantity 3.3%,pH 8.0.The release rate of ferulic acid reached 14.55% which was increased by 106.7% compared with that of unoptimized conditions.The synergistic effect of mixed enzymes containing ferulic acid esterase(FAE) and xylanase which were used to hydrolyze destarched rice bran.The release rate of FA reached 20.22%,which was increased by 119.1% compared with enzymatic hydrolysis of rice bran by ferulic acid esterase only.The release amount of xylooligosaccharides reached 2.04 mg·mL-1,which was increased 87.2% compared with enzymatic hydrolysis of rice bran by xylanase only.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026,20446004); 福建省科技计划重点项目(20031020)
更新日期/Last Update: 2014-03-23