[1]张亚武,肖美添,叶静,等.醇沉-萃取法提取(2S,5S)-2,5-己二醇[J].华侨大学学报(自然科学版),2010,31(2):196-199.[doi:10.11830/ISSN.1000-5013.2010.02.0196]
　ZHANG Ya-wu,XIAO Mei-tian,YE Jing,et al.Extraction of (2S,5S)-2,5-Hexanediol Via a Combination of Alcohol Precipitation and Extraction[J].Journal of Huaqiao University(Natural Science),2010,31(2):196-199.[doi:10.11830/ISSN.1000-5013.2010.02.0196]
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醇沉-萃取法提取(2S,5S)-2,5-己二醇()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第31卷
期数:
2010年第2期
页码:
196-199
栏目:
出版日期:
2010-03-20

文章信息/Info

Title:
Extraction of (2S,5S)-2,5-Hexanediol Via a Combination of Alcohol Precipitation and Extraction
文章编号:
1000-5013(2010)02-0196-04
作者:
张亚武; 肖美添; 叶静; 黄雅燕
华侨大学化工学院
Author(s):
ZHANG Ya-wu;  XIAO Mei-tian;  YE Jing;  HUANG Ya-yan
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
(2S; 5S)-2; 5-己二醇;  醇沉;  萃取;  发酵液
Keywords:
(2S; 5S)-2; 5-hexanediol;  alcohol precipitation;  extraction;  fermentation broth
分类号:
TQ223.162
DOI:
10.11830/ISSN.1000-5013.2010.02.0196
文献标志码:
A
摘要:
利用醇沉-萃取法,从生物转化发酵液中提取(2S,5S)-2,5-己二醇,并考察醇沉、萃取和结晶的工艺条件.结果表明,醇沉时,加入的乙醇与浓缩发酵液的最佳体积比为2∶1,pH值对醇沉效果影响显著,强碱和强酸条件下可使大部分杂质析出.经乙酸乙酯连续5次萃取,醇沉浓缩液中2,5-己二醇的萃取率达75.0%.通过优化后的工艺进行分离纯化,发酵液中2,5-己二醇总提取率为49.2%,纯度≥98.0%,(2S,5S)-2,5-己二醇的光学纯度值在提纯前后均为94.5%.
Abstract:
The technological conditions of extracting(2S,5S)-2,5-hexanediol from fermentation broth by a combination of alcohol precipitation and extraction was investigated.The refined product 2,5-hexanediol was obtained from fermentation broth by centrifugation,alcohol precipitation.extraction,crystallization and recrystallization.The results showed the optimal volume ratio of alcohol to condensed fermentation broth in alcohol precipitation was 2∶1.The pH value of broth had great effect on precipitation,most impuritis could be precipitated under strong acidity or basicity condition.And,after five consecutive extractions from concentrated liquid with ethyl acetate,the yield of 2,5-hexanediol could reach 75.0%.By using the optimal process of separation and purification from fermentation broth,the yield of 2,5-hexanediol was 49.2%,and its purity could be up to 98.0%.The optical purity value of the refined product(2S,5S)-2,5-hexanediol could be kept at 94.5% after purification process.

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备注/Memo

备注/Memo:
福建省泉州市科技计划重点项目(2006G05)
更新日期/Last Update: 2014-03-23