[1]吕英海,周仕学,李桂江,等.不同缓冲体系的牛血清白蛋白插层蒙脱石效果[J].华侨大学学报(自然科学版),2009,30(6):661-664.[doi:10.11830/ISSN.1000-5013.2009.06.0661]
　Lv Ying-hai,ZHOU Shi-xue,LI Gui-jiang,et al.Effect of Intercalation of Bovine Serum Albumin into Montmorillonite in Different Buffer Systems[J].Journal of Huaqiao University(Natural Science),2009,30(6):661-664.[doi:10.11830/ISSN.1000-5013.2009.06.0661]
点击复制

不同缓冲体系的牛血清白蛋白插层蒙脱石效果()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第6期
页码:
661-664
栏目:
出版日期:
2009-11-20

文章信息/Info

Title:
Effect of Intercalation of Bovine Serum Albumin into Montmorillonite in Different Buffer Systems
文章编号:
1000-5013(2009)06-0661-04
作者:
吕英海; 周仕学; 李桂江; 王斌; 吕东琴
山东科技大学化学与环境工程学院
Author(s):
Lv Ying-hai;  ZHOU Shi-xue;  LI Gui-jiang;  WANG Bin;  L Dong-qin
College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, China
关键词:
蒙脱石;  牛血清白蛋白;  插层;  pH值;  缓冲液
Keywords:
montmorillonite;  bovine serum albumin;  intercalation;  pH value;  buffer
分类号:
TB383.1
DOI:
10.11830/ISSN.1000-5013.2009.06.0661
文献标志码:
A
摘要:
利用离子交换的原理,研究等电点两侧的不同pH值、不同缓冲体系对牛血清白蛋白插层蒙脱石效果的影响.结果发现,pH值对蒙脱石插层影响显著.在采用低于蛋白质等电点的pH值且利用乙酸-乙酸钠作为缓冲体系时,蛋白质的插层容易造成蒙脱石层间的剥离.等电点两侧的pH值不同,会造成蛋白质插入量的显著差异.热重分析表明,蛋白质/蒙脱石复合物的热稳定性有一定程度的提高.
Abstract:
By applying the principle of ion exchange,the effect of intercalation of bovine serum albumin(BSA) into montmorillonite was investigated under the different pH near isoelectric point of BSA and impact of different buffer.It was found that pH value significantly affects the intercalation of the BSA.A pH lower than the isoelectric point together with the use of acetic acid-sodium acetate buffer system is often helpful to cause the spin-off of montmorillonite.The weight of BSA inserted exhibits a notable difference with different pH value and the thermal stability of protein/montmorillonite complex has been improved to some extent.

参考文献/References:

[1] JUDEINSTEIN P, SANCHEZ C. Hybrid organic-inorganic materials:A land of multi-disciplinarity [J]. Journal of Materials Chemistry, 1996(4):511-525.
[2] CHANDROSS E A, MILLER R D. Nanostructures:Introduction [J]. Chemical Reviews, 1999(7):1641-1642.
[3] 洪伟良, 刘剑洪, 田德余. 有机-无机纳米复合材料的制备方法 [J]. 化学研究与应用, 2000(2):132-136.doi:10.3969/j.issn.1004-1656.2000.02.003.
[4] 王一中. 聚合物/蒙脱土混杂材料的制备及其表征 [D]. 北京:北京化工大学, 1999.
[5] LIN J J, WEI J C, JUANG T Y. Preparation of protein-silicate hybrids from polyamine intercalation of layered montmorillonite [J]. Langmuir, 2007(4):1995-1999.doi:10.1021/la062013h.
[6] CHEN Guo-jing, YEN Ming-cheng, WANG Jen-ming. Layered inorganic/enzyme nanohybrids with selectivity and structural stability upon interacting with biomolecules [J]. Bioconjugate Chemistry, 2008(1):138-144.
[7] 郭蔼光, 郭泽坤. 生物化学实验技术 [M]. 北京:高等教育出版社, 2007.60-62.
[8] LOZZI I, PUCCI A, PANTANI O L. Interferences of suspended clay fraction in protein quantitation by several determination methods [J]. Analytical Biochemistry, 2008(1):108-114.
[9] ZHAO Fang-ming, XIONG Chuan-xi, WEN Di-jiang. Role of tough interfacial layers in fibre reinforced composites [J]. Journal of Wuhan University of Technology, 1996(2):38-43.
[10] 李纯毅, 李赞忠, 付渊. 红外光谱在二茂铁类化合物中的应用 [J]. 内蒙古石油化工, 2008(1):27-29.doi:10.3969/j.issn.1006-7981.2008.01.011.

相似文献/References:

[1]叶玲,黄继泰,戴劲草,等.离子交换条件对蒙脱石表面酸性的影响[J].华侨大学学报(自然科学版),1998,19(4):371.[doi:10.11830/ISSN.1000-5013.1998.04.0371]
[2]叶玲.配位化合物改性蒙脱石的结构及吸附性能[J].华侨大学学报(自然科学版),2006,27(1):50.[doi:10.3969/j.issn.1000-5013.2006.01.013]
　Ye Ling.Structure and Adsorption Performance of Montmorillonite Modified by Coordination Compounds[J].Journal of Huaqiao University(Natural Science),2006,27(6):50.[doi:10.3969/j.issn.1000-5013.2006.01.013]
[3]叶玲,张敬阳,肖子敬.改性蒙脱石的电动性质及吸附直接大红染料性能[J].华侨大学学报(自然科学版),2018,39(3):389.[doi:10.11830/ISSN.1000-5013.201711074]
　YE Ling,ZHANG Jingyang,XIAO Zijing.Zeta-Potential of Modified Montmorillonite and Its Effect on Adsorption Capacity for Direct Red Dye From Aqueous Solutions[J].Journal of Huaqiao University(Natural Science),2018,39(6):389.[doi:10.11830/ISSN.1000-5013.201711074]

备注/Memo

备注/Memo:
国家自然科学基金资助项目(50574054); 山东科技大学春蕾计划(2008AZZ075)
更新日期/Last Update: 2014-03-23