[1]李红然,贺淹才,刘治江,等.产几丁质酶菌株的分离与鉴定及产酶条件优化[J].华侨大学学报(自然科学版),2009,30(5):538-542.[doi:10.11830/ISSN.1000-5013.2009.05.0538]
　LI Hong-ran,HE Yan-cai,LIU Zhi-jiang,et al.Isolation,Identification of Chitinase Producing Microorganism and Optimization of Its Fermentation Conditions[J].Journal of Huaqiao University(Natural Science),2009,30(5):538-542.[doi:10.11830/ISSN.1000-5013.2009.05.0538]
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产几丁质酶菌株的分离与鉴定及产酶条件优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第5期
页码:
538-542
栏目:
出版日期:
2009-09-20

文章信息/Info

Title:
Isolation,Identification of Chitinase Producing Microorganism and Optimization of Its Fermentation Conditions
文章编号:
1000-5013(2009)05-0538-05
作者:
李红然; 贺淹才; 刘治江; 刘杨斐
华侨大学工业生物技术福建省高等学校重点实验室
Author(s):
LI Hong-ran;  HE Yan-cai;  LIU Zhi-jiang;  LIU Yang-fei
Key Laboratory of Industrial Biotechnology of Fujian Drovince University, Huaqiao University, Quanzhou 362021, China
关键词:
几丁质酶;  Luteibacter rhizovicinus;  优化;  酶活力
Keywords:
chitinase;  Luteibacter rhizovicinus;  optimization;  enzyme activity
分类号:
X172
DOI:
10.11830/ISSN.1000-5013.2009.05.0538
文献标志码:
A
摘要:
从土壤中分离到一株产几丁质酶的细菌LL-C.该菌经16S rDNA序列同源性分析及形态培养特征、生理生化特征分析,鉴定为Luteibacter rhizovicinus,属于黄单胞菌科(Xanthomonadaceae).以不同发酵时间、碳源、氮源、起始pH值、培养基装液量和培养温度为因素,考察LL-C菌株产几丁质酶的优化条件.结果表明,最有利于该菌产几丁质酶的条件:碳源为胶体几丁质、氮源为(NH4)2SO4,培养基起始pH值为4.5,培养基装液量为30 mL,培养温度为32℃,振荡培养时间为7 d,此优化条件下,摇瓶产酶活力为115.02μkat.L-1.
Abstract:
A chitinase producing strain LL-C was isolated from soil.According to the analysis of the 16S rDNA sequence,the cultivation condition,its morphology and physiological characteristics of the LL-C,it was identified as Luteibacter rhizovicinus belong to Xanthomonadaceae.The effects of a range of factors on the production of chitinase of the LL-C were studied,including time,carbon source,nitrogen source,pH,volume of medium and cultural temperature.Experimental results showed that the optimal carbon and nitrogen source were colloid chitin and(NH4)2SO4 respectively.The optimal cultivation conditions for LL-C producing chitinase were 250 mL flask containing 30 mL medium with initial pH 4.5,30 ℃ incubating temperature and shaking cultivation for 7 days.Under optimized conditions,the chitinase activity produced by LL-C could reach 115.02 μkat·L-1.

参考文献/References:

[1] TAKEOMI M, SATOSHI A, TAKESHI H. Purification and characterization of a chitinase from Amycolatopsis orientalis with N-acetyllactosamine-repeating unit releasing activity [J]. Biochemical and Biophysical Research Communications, 2005(2):514-520.doi:10.1016/j.bbrc.2005.08.123.
[2] ROCO A, PEREZ L M. In vitro biocontrol activity of Trichoderma harzianum on Alternaria alternata in the presence of growth regulators [J]. Electronic Journal of Biotechnology, 2001(2):68-73.
[3] PANKAJ P, DEEPTI A, TUSHAR B. Optimisation of process parameters for chitinase productionby soil isolates of Penicillium chrysogenum under solid substrate fermentation [J]. Process Biochemistry, 2004(9):2962-2967.
[4] 沈萍, 范秀容, 李广武. 微生物学实验 [M]. 北京:高等教育出版社, 1999.
[5] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册 [M]. 北京:科学出版社, 2001.
[6] WEN P C, TSONG T K. A simple and rapid method for the preparation of gram-negative bacterial genomic DNA [J]. Nucleic Acids Research, 1993(9):2260-2263.doi:10.1093/nar/21.9.2260.
[7] 袁勇军, 陆兆新, 黄丽金. 烟碱降解细菌的分离、鉴定及其降解性能的初步研究 [J]. 微生物学报, 2005(2):181-184.doi:10.3321/j.issn:0001-6209.2005.02.005.
[8] JOSHI S, KOZLOWSKI M, RICHENS S. Chitinase and chitobiase production during Fermenta-tion of genetically improved Serratia liquefaciens [J]. Enzyme and Microbial Technology, 1989(5):289-296.doi:10.1016/0141-0229(89)90044-6.
[9] 都立辉, 刘芳. 16S rRNA基因在细菌菌种鉴定中的应用 [J]. 乳业科学与技术, 2006(5):207-209.doi:10.3969/j.issn.1671-5187.2006.05.001.
[10] JOHANSEN J E, BINNERUP S J, KROER N. Luteibacter rhizovicinus gen nov, sp.nov, a yellow-pigmented gammaproteobacterium Isolated from the rhizosphere of barley (Hordeum vulgare L.) [J]. Systematic and Evolutionary Microbiology, 2005(6):2285-2291.doi:10.1099/ijs.0.63497-0.
[11] ANDERSEN O A, DIXON M J, EGGLESTON I M. Natural product family 18 chitinase inhibitors [J]. Natural Product Reports, 2005(5):563-579.doi:10.1039/b416660b.
[12] IZUMIDA H, IMAMURA N, SANO H. A novel chitinase inhibitor from a marine bacterium, Pseudomonas sp [J]. J Antibiotic(Tokyo), 1996(1):76-80.
[13] 邱力友, 王明道, 戚远成. 链霉菌A048产几丁质酶最佳发酵工艺研究 [J]. 微生物学通报, 2006(2):58-62.doi:10.3969/j.issn.0253-2654.2006.02.013.
[14] NAWANI N N, KAPADNIS B P. Optimization of chitinase production using statistics based experimental designs [J]. Process Biochemistry, 2005(2):651-660.doi:10.1016/j.procbio.2004.01.048.
[15] KAO Po-min, CHEN Chih-i, HUANG Shu-chen. Effects of shear stress and mass transfer on chitinase production by Paenibacillus sp.CHE-N1 [J]. Biochemical Engineering Journal, 2007(2):172-178.doi:10.1016/j.bej.2006.11.028.
[16] 贺淹才, 刘爱花, 张荣奎. 嗜麦芽窄食单胞菌产生的几丁质酶的特性 [J]. 华侨大学学报(自然科学版), 2008(2):245-249.
[17] 贺淹才, 刘爱花, 柴思捷. 改进的RBB染色法初步筛选几丁质酶基因chiA的表达 [J]. 华侨大学学报(自然科学版), 2008(4):563-566.
[18] 李红然, 贺淹才, 刘治江. 几丁质酶抑制剂的研究进展 [J]. 生物学通报, 2007(1):8-10.doi:10.3969/j.issn.0006-3193.2007.01.004.

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(C04010011)
更新日期/Last Update: 2014-03-23