[1]刘艳锋,周作明,李小林,等.芘降解菌的分离纯化及其降解性能测定[J].华侨大学学报(自然科学版),2008,29(2):267-269.[doi:10.11830/ISSN.1000-5013.2008.02.0267]
　LIU Yan-feng,ZHOU Zuo-ming,LI Xiao-lin,et al.Isolation and Purification of Pyrene Degradating Strains and Measurement of their Degradation Capability[J].Journal of Huaqiao University(Natural Science),2008,29(2):267-269.[doi:10.11830/ISSN.1000-5013.2008.02.0267]
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芘降解菌的分离纯化及其降解性能测定()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第2期
页码:
267-269
栏目:
出版日期:
2008-04-20

文章信息/Info

Title:
Isolation and Purification of Pyrene Degradating Strains and Measurement of their Degradation Capability
文章编号:
1000-5013(2008)02-0267-03
作者:
刘艳锋; 周作明; 李小林; 荆国华; 方柏山
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021
Author(s):
LIU Yan-feng;  ZHOU Zuo-ming;  LI Xiao-lin;  JING Guo-hua;  FANG Bai-shan
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
芘;  降解菌;  分离;  纯化;  生物降解
Keywords:
pyrene;  degradating strains;  isolation;  purification;  microbiological degradation
分类号:
X172
DOI:
10.11830/ISSN.1000-5013.2008.02.0267
文献标志码:
A
摘要:
通过对某炼油厂生化反应池的活性污泥的富集与驯化,得到一组能以芘为唯一碳源的混合微生物DP.将混和菌DP分离纯化,可得到6种芘降解菌DP1,DP2,DP3,DP4,DP5和DP6.通过显微镜观察各降解菌的菌落特征及革兰氏染色实验,初步判断DP1为细菌,其他为放线菌.在芘质量浓度为100 mg.L-1的培养基中培养44 h后,6种菌对芘的降解率分别为46.2%,83.2%,23.6%,11.3%,53.3%,13.6%,其中DP2为芘的高效降解菌.
Abstract:
A microbial consortium named DP was cultivated from the refinery activated sludge with pyrene as the single carbon and energey source.Six pure strains were isolated and purified from DP,which were named as DP1,DP2,DP3,DP4,DP5 and DP6.DP1 was a specy of bacteria and the others were species of actinomycetes according to morphological observations,including colony observation and light microscope examination basing on the Gram staining of the strains.To measure their degradation capability,the removal efficiencies of pyrene were 46.2%,83.2%,23.6%,11.3%,53.3%and 13.6% respectively after 44 hours in the mineral salts medium under initial pyrene concentration of 100 mg·L-1.DP2 was a kind of high-efficient degradating strain towards pyrene.

参考文献/References:

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(D0710019); 国务院侨办科研基金资助项目(06QZR09)
更新日期/Last Update: 2014-03-23