[1]岑传铨.海藻酸钡固定化尿酶电极的研制及应用血清中尿素含量的安培测定[J].华侨大学学报(自然科学版),1991,12(4):440-445.[doi:10.11830/ISSN.1000-5013.1991.04.0440]
　Cen Chuanquan.An Urease Electrode Prepared by Barium Alginate Immobilizing and Applied to Amperometric Determination of Serum Urea[J].Journal of Huaqiao University(Natural Science),1991,12(4):440-445.[doi:10.11830/ISSN.1000-5013.1991.04.0440]
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海藻酸钡固定化尿酶电极的研制及应用血清中尿素含量的安培测定()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第12卷
期数:
1991年第4期
页码:
440-445
栏目:
出版日期:
1991-10-20

文章信息/Info

Title:
An Urease Electrode Prepared by Barium Alginate Immobilizing and Applied to Amperometric Determination of Serum Urea
作者:
岑传铨
华侨大学应用化学系
Author(s):
Cen Chuanquan
关键词:
尿素;  酶电极;  固定化酶;  血清;  尿酶电极
Keywords:
enzyme electrodes;  immobilized enzyme;  urease electrode;  serum;  urea nitrogen
DOI:
10.11830/ISSN.1000-5013.1991.04.0440
摘要:
本文报道了用海藻酸钡固定干粉状的尿素酶,并制成酶电极。文中对背景液,选择在 lmmol/L NaH2PO4,lmmol/L Na2HPO4,lmmol/L EDTA,0.1mol/L KCI 10mmol/L 硫酸肼经 NaOH 溶液调节至 pH=7.0的背景液中测定。其电流值与尿素浓度在6×10-5—4×10-3mol/L 间成线性关系,检测限为2×10-5mol/L。本电极以安培法测定血清中尿素氮含量并与尿酶比色法进行比较结果较为满意。经100多次测定电极寿命约10多天,电极的稳定性和重现性良好。
Abstract:
An enzyme electrode was prepared by immobilizing the dry powdered urease with barium al- ginate and was applied to the amperometric determination of serum urea nitrogen.The solution contain- ing 1m mol/L NaH2PO4,1m mol/L Na2HPO4,1m mol/L EDTA,0.1mol/L KCl

相似文献/References:

[1]岑传铨,庄秀润.PVA固定化葡萄糖氧化酶电极的研制及应用——血清中葡萄糖含量的测定[J].华侨大学学报(自然科学版),1989,10(4):387.[doi:10.11830/ISSN.1000-5013.1989.04.0387]
　Cen Chuanquan,Zhuang Xiurun.Preparation of PVA Immobilized Glucose Oxidase Electrode and ItS Application in Serum Glucose Determination[J].Journal of Huaqiao University(Natural Science),1989,10(4):387.[doi:10.11830/ISSN.1000-5013.1989.04.0387]

备注/Memo

备注/Memo:
1988年福建省自然科学基金课题.
更新日期/Last Update: 2014-03-22