[1]成志云,陈佳怡,白如玉,等.采用Cell-SELEX技术的核酸适配体在肿瘤靶向治疗的研究进展[J].华侨大学学报(自然科学版),2024,45(4):439-444.[doi:10.11830/ISSN.1000-5013.202401016]
 CHENG Zhiyun,CHEN Jiayi,BAI Ruyu,et al.Research Progress of Aptamers Using Cell-SELEX Technology in Cancer Targeted Treatment[J].Journal of Huaqiao University(Natural Science),2024,45(4):439-444.[doi:10.11830/ISSN.1000-5013.202401016]
点击复制

采用Cell-SELEX技术的核酸适配体在肿瘤靶向治疗的研究进展()
分享到:

《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第4期
页码:
439-444
栏目:
出版日期:
2024-07-20

文章信息/Info

Title:
Research Progress of Aptamers Using Cell-SELEX Technology in Cancer Targeted Treatment
文章编号:
1000-5013(2024)04-0439-06
作者:
成志云 陈佳怡 白如玉 杨会勇 MOHSAN Ullah 刁勇
华侨大学 医学院, 福建 泉州 362021
Author(s):
CHENG Zhiyun CHEN Jiayi BAI Ruyu YANG Huiyong MOHSAN Ullah DIAO Yong
School of Medicine, Huaqiao University, Quanzhou 362021, China
关键词:
核酸适配体 细胞-配体指数富集系统进化(Cell-SELEX)技术 肿瘤 靶向治疗
Keywords:
aptamers cell-systematic evolution of ligands by exponential enrichment(Cell-SELEX)technology cancer targeted treatment
分类号:
R944
DOI:
10.11830/ISSN.1000-5013.202401016
文献标志码:
A
摘要:
阐述细胞-配体指数富集系统进化(Cell-SELEX)技术特点,以及通过该技术筛选得到的核酸适配体在肿瘤靶向治疗中的应用进展和挑战,通过查阅近年的相关文献,综述核酸适配体作为药物及药物载体在肿瘤靶向治疗中的应用研究进展。结果表明:基于Cell-SELEX技术筛选得到的核酸适配体在肿瘤靶向治疗中的疗效显著,可开发成为肿瘤靶向治疗的潜力药物及良好的药物载体。
Abstract:
The characteristics of cell-systematic evolution of ligands by exponential enrichment(Cell-SELEX)technology as well as the application progress and challenges of screened aptamers in cancer-targeted treatment through this technology were expounded. And the research progress about cancer-targeted treatment applications of aptamers as drugs or drug carriers were reviewed by consulting relevant literature in recent years. The results show that the aptamers screened based on Cell-SELEX technology have significant therapeutic effects and can be developed as potential drugs and good drug carriers in cancer-targeted treatment.

参考文献/References:

[1] NG E W M,SHIMA D T,CALIAS P,et al.Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease[J].Nature Reviews Drug Discovery,2006,5(2):123-132.DOI:10.1038/nrd1955.
[2] BING Tao,SHANGGUAN Dihua,WANG Yinsheng.Facile discovery of cell-surface protein targets of cancer cell aptamers[J].Molecular and Cellular Proteomics,2015,14(10):2692-2700.DOI:10.1074/mcp.m115.051243.
[3] ZHOU Wei,ZHAO Libo,YUAN Hongyu,et al.A new small cell lung cancer biomarker identified by Cell-SELEX generated aptamers[J].Experimental Cell Research,2019,382(2/3):111478.DOI:10.1016/j.yexcr.2019.06.023.
[4] LYU Jing,ZHEN Xiaoxiao,LI Dandan,et al.Generation of an aptamer targeting receptor-type tyrosine protein phosphatase F[J].Analytical Chemistry,2023,95(2):1228-1233.DOI:10.1021/acs.analchem.2c03988.
[5] ZAMAY G S,KOLOVSKAYA O S,ZAMAY T N,et al.Aptamers selected to postoperative lung adenocarcinoma detect circulating tumor cells in human blood[J].Molecular Therapy,2015,23(9):1486-1496.DOI:10.1038/mt.2015.108.
[6] WU Xiaoqiu,LIU Honglin,HAN Dongmei,et al.Elucidation and structural modeling of CD71 as a molecular target for cell-specific aptamer binding[J].Journal of the American Chemical Society,2019,141(27):10760-10769.DOI:10.1021/jacs.9b03720.
[7] JIA Wenting,REN Caiping,WAN Lei,et al.CD109 is identified as a potential nasopharyngeal carcinoma biomarker using aptamer selected by cell-SELEX[J].Oncotarget,2016,7(34):55328-55342.DOI:10.18632/oncotarget.10530.
[8] WANG Guodong,LIU Jun,CHEN Ke,et al.Selection and characterization of DNA aptamer against glucagon receptor by cell-SELEX[J].Scientific Reports,2017,7(1):7179.DOI:10.1038/s41598-017-05840-w.
[9] CALZADA V,MORENO M,NEWTON J,et al.Development of new PTK7-targeting aptamer-fluorescent and radiolabelled probes for evaluation as molecular imaging agents: Lymphoma and melanoma in vivo proof of concept[J].Bioorganic and Medicinal Chemistry,2017,25(3):1163-1171.DOI:10.1016/j.bmc.2016.12.026.
[10] DHARMASIRI U,BALAMURUGAN S,ADAMS A A,et al.Highly efficient capture and enumeration of low abundance prostate cancer cells using prostate-specific membrane antigen aptamers immobilized to a polymeric microfluidic device[J].Electrophoresis,2009,30(18):3289-3300.DOI:10.1002/elps.200900141.
[11] XU Ye,PHILLIPS J A,YAN Jilin,et al.Aptamer-based microfluidic device for enrichment, sorting, and detection of multiple cancer cells[J].Analytical Chemisitry,2009,81(17):7436-7442.DOI:10.1021/ac9012072.
[12] 刁勇,王立强,邱飞.抗肿瘤适体药物的研究进展[J].中国生化药物杂志,2009,30(6):415-418.
[13] RONG Yuan,CHEN Hao,ZHOU Xuefeng,et al.Identification of an aptamer through whole cell-SELEX for targeting high metastatic liver cancers[J].Oncotarget,2016,7(7):8282-8294.DOI:10.18632/oncotarget.6988.
[14] CERCHIA L,DUCONGé F,PESTOURIE C,et al.Neutralizing aptamers from whole-cell SELEX inhibit the RET receptor tyrosine kinase[J].Plos Biology,2005,3(4):697-704.DOI:10.1371/journal.pbio.0030123.
[15] ZUEVA E,RUBIO L I,DUCONGé F,et al.Metastasis-focused cell-based SELEX generates aptamers inhibiting cell migration and invasion[J].International Jouranl of Cancer,2011,128(4):797-804.DOI:10.1002/ijc.25401.
[16] ESPOSITO C L,PASSARO D,LONGOBARDO I,et al.A neutralizing RNA aptamer against EGFR causes selective apoptotic cell death[J].Plos One,2011,6(9):e24071.DOI:10.1371/journal.pone.0024071.
[17] MAHLKNECHT G,MARON R,MANCINI M,et al.Aptamer to ErbB-2/HER2 enhances degradation of the target and inhibits tumorigenic growth[J].Proceedings of the National Acadmey of Sciences of the United States of Americal,2013,110(20):8170-8175.DOI:10.1073/pnas.1302594110.
[18] KOLOVSKAYA O S,ZAMAY T N,ZAMAY A S,et al.DNA-aptamer/protein interaction as a cause of apoptosis and arrest of proliferation in ehrlich ascites adenocarcioma cells[J].Biologicheskie Membrany,2013,30(5/6):398-411.DOI:10.7868/S023347551305006X.
[19] CAMORANI S,ESPOSITO C L,RIENZO A,et al.Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRβ aptamer[J].Molecular Therapy,2014,22(4):828-841.DOI:10.1038/mt.2013.300.
[20] ESPOSITO C L,VAN ROOSBROECK K,SANTAMARIA G,et al.Selection of a nuclease-resistant RNA aptamer targeting CD19[J].Cancers,2021,13(20):5220.DOI:10.3390/cancers13205220.
[21] MERAVIGLIA-CRIVELLI D,VILLANUEVA H,MENON A P,et al.A pan-tumor-siRNA aptamer chimera to block nonsense-mediated mRNA decay inflames and suppresses tumor progression[J].Molecular Therapy-Nucleic Acids,2022,29:413-425.DOI:10.1016/j.omtn.2022.07.017.
[22] KELLY L,KRATSCHMER C,MAIER K E,et al.Improved synthesis and in vitro evaluation of an aptamer ribosomal toxin conjugate[J].Nucleic Acid Therapeutics,2016,26(3):156-165.DOI:10.1089/nat.2015.0599.
[23] QIAO Yan,SHI Yanli,JI Mengmeng,et al.Selection and identification of a prohibitin 2-binding DNA aptamer for tumor tissue imaging and targeted chemotherapy[J].International Journal of Biological Macromolecules,2024,259:129002.DOI:10.1016/j.ijbiomac.2023.129002.
[24] GAO Fei,YIN Jianhui,CHEN Yan,et al.Recent advances in aptamer-based targeted drug delivery systems for cancer therapy[J].Frontiers in Bioengineering and Biotechnology,2022,10:972933.DOI:10.3389/fbioe.2022.972933
[25] DUAN Tao,XU Zhuobin,SUN Fumo,et al.HPA aptamer functionalized paclitaxel-loaded PLGA nanoparticles for enhanced anticancer therapy through targeted effects and microenvironment modulation[J].Biomedicine and Pharmacothrapy,2019,117:109121.DOI:10.1016/j.biopha.2019.109121.
[26] MANOOCHEHRI H,JALALI A,TANZADEHPANAH H,et al.Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs[J].Colloids and Surfaces B: Biointerfaces,2022,218:112714.DOI:10.1016/j.colsurfb.2022.112714.
[27] MEHRNIA S S,HASHEMI B,MOWLA S J,et al.Radiosensitization of breast cancer cells using AS1411 aptamer-conjugated gold nanoparticles[J].Radiation Oncology,2021,16(1):33.DOI:10.1186/s13014-021-01751-3.
[28] WANG Tengfei,LUO Yu,LYU Haiyin,et al.Aptamer-based erythrocyte-derived mimic vesicles loaded with siRNA and DOX for the targeted treatment of multidrug resistance tumors[J].ACS Applied Materials and Interface,2019,11(49):45455-45466.DOI:10.1021/acsami.9b16637.
[29] BOLTZ A,PIATER B,TOLEIKIS L,et al.Bi-specific aptamers mediating tumor cell lysis[J].Journal of Biological Chemisty,2011,286(24):21896-21905.DOI:10.1074/jbc.M111.238261.
[30] ZHU Huijie,LI Jin,ZHANG Xiaobin,et al.Nucleic acid aptamer-mediated drug delivery for targeted cancer therapy[J].Chemmedchem,2014,10(1):39-45.DOI:10.1002/cmdc.201402312.
[31] ZHONG Yi, ZHAO Jiayao,LI Jiazhao,et al.Advances of aptamers screened by Cell-SELEX in selection procedure, cancer diagnostics and therapeutics[J].Analytical Biochemistry,2020,598:113620.DOI:10.1016/j.ab.2020.113620.

备注/Memo

备注/Memo:
收稿日期: 2024-01-29
通信作者: 刁勇(1967-),男,教授,博士,博士生导师,主要从事基因药物的研究。E-mail:diaoyong@hqu.edu.cn。
基金项目: 国家自然科学基金资助项目(20141ZA003); 福建省泉州市高层次人才创新项目(20221ZC061)
更新日期/Last Update: 2024-07-20