[1]王忠敏,黄惠莉.一株罗非鱼出血病致病菌的分离与鉴定及组织病理观察[J].华侨大学学报(自然科学版),2012,33(6):660-666.[doi:10.11830/ISSN.1000-5013.2012.06.0660]
　WANG Zhong-min,HUANG Hui-li.Isolation, Identification and Histopathologic Observation of a Pathogen from Hemorrhage Tilapia[J].Journal of Huaqiao University(Natural Science),2012,33(6):660-666.[doi:10.11830/ISSN.1000-5013.2012.06.0660]
点击复制

一株罗非鱼出血病致病菌的分离与鉴定及组织病理观察()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第33卷
期数:
2012年第6期
页码:
660-666
栏目:
出版日期:
2012-11-20

文章信息/Info

Title:
Isolation, Identification and Histopathologic Observation of a Pathogen from Hemorrhage Tilapia
文章编号:
1000-5013(2012)06-0660-07
作者:
王忠敏;  黄惠莉
华侨大学 化工学院, 福建 厦门 361021
Author(s):
WANG Zhong-min;  HUANG Hui-li
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
罗非鱼;  蜡状芽孢杆菌;  出血病;  组织切片;  病理
Keywords:
tilapia;  Bacillus cereus;  hemorrhage;  tissue section;  pathology
分类号:
Q939.124
DOI:
10.11830/ISSN.1000-5013.2012.06.0660
文献标志码:
A
摘要:
从患有出血病症状的罗非鱼病灶部位分离筛选出一株高致病性菌(编号为BC-2),结合对菌落形态观察、生理生化实验及16S rDNA测序,确定该高致病菌为一种致罗非鱼死亡的蜡状芽孢杆菌.通过对健康鱼进行不同浓度致病菌的感染试验及定性数据机率的分析,可知其半数致死量(LD50)的对数值为7.534 8、感染的菌浓度为3.4×107 cfu·mL-1、置信限为95%.通过对患病鱼的不同组织器官的切片病理结构观察,可以发现鳃和肠是病变最明显的部位.
Abstract:
A highly pathogenic bacterium(No. BC-2)was isolated from the infected hemorrhage tilapia. Combination of the colonial morphology observation, the biochemistry test and 16S rDNA sequencing, the highly pathogenic bacterium was determined as Bacillus cereus, which could cause tilapia dead. Then infective test was carried out to normal tilapias with different concentrations of the pathogen, and the qualitative probability data analysis results showed that the logarithmic concentration of LD50 was 7.534 8 with the confidence interval 95% when the infective concentration was 3.4×107 cfu·mL-1. After observing the pathological structure of different tissue and organ sections from infected tilapia, we found gill and intestine were the most evident lesion parts.

参考文献/References:

[1] 曹维国.常见尼罗罗非鱼病的诊治[J].中国水产,1984(2):24-25.
[2] 李槿年,沈守琼,余为一,等.正常鱼体内条件致病菌种类的调查研究[J].淡水渔业,1999(2):21-24.
[3] 邓国成,江小燕,叶星,等.草鱼出血病混合感染的嗜水气单胞菌的分离、鉴定与理化特性[J].微生物学通报,2009,36(8):1170-1173.
[4] 卢迈新,黎炯,叶星,等.广东与海南养殖罗非鱼无乳链球菌的分离、鉴定与特性分析[J].微生物学通报,2010,37(5):766-774.
[5] FIGUEIREDO H C P,KLESIUS P H,ARIAS C R,et al.Isolation and characterization of strains of Flavobacterium columnare from Brazil[J].Journal of Fish Diseases,2005,28(4):199-204.
[6] EVANS J J,KLESIUS P H,PASNIK D J,et al.Human streptococcus agalactiae Isolate in nile tilapia(Oreochromis niloticus)[J].Emerging Infectious Diseases,2009,15(5):774-776.
[7] SOTO E,HAWKE J P,FERNANDEZ D,et al.Francisella sp., an emerging pathogen of tilapia, Oreochromis niloticus(L.), in Costa Rica[J].Journal of Fish Diseases,2009,32(8):713-722.
[8] NA J S,KIM T H,KIM H S,et al.Liver abscess and sepsis with Bacillus pantothenticus in an immunocompetent patient: A first case report[J].World Journal of Gastroenterology, 2009,15(42):5360-5363.
[9] 冯斯敏,云金蕊.蜡状芽胞杆菌引起军曹鱼大批死亡的报告[J].中国水产,2000(8):39-40.
[10] 彭喜春,张宁,冉艳红,等.海产品中溶藻弧菌的筛选及其致病因子的研究[J].现代食品科技,2008,24(4):312-315.
[11] 刘云,刘允坤,杨栋,等.牙鲆迟缓爱德华氏菌急性感染实验: 4种不同方法的比较[J].海洋科学,2001,25(2):8-11.
[12] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[13] FINNEY D J.Probit analysis[M].Cambridge:Cambridge University Press,1977.
[14] ABEE T,GROOT M N,TEMPELAARS M,et al.Germination and outgrowth of spores of Bacillus cereus group members: Diversity and role of germinant receptors[J].Food Microbiology,2011,28(2):199-208.
[15] BEDNARCZYK A,DACZKOWSKA-KOZON E G.Pathogenic features of bacteria from the Bacillus cereus group[J].Postepy Mikrobiologii,2008,47(1):51-63.
[16] CARLIN F,BRILLARD J,BROUSSOLLE V,et al.Adaptation of Bacillus cereus, an ubiquitous worldwide-distributed foodborne pathogen, to a changing environment[J].Food Research International,2010,43(7):1885-1894.
[17] ARAGON-ALEGRO L C,PALCICH G,LOPES G V,et al.Enterotoxigenic and genetic profiles of Bacillus cereus strains of food origin in Brazil[J].Journal of Food Protection,2008,71(10):2115-2118.
[18] 施彪,戚爱军.蜡样芽孢杆菌引起败血症1例[J].淮海医药,1999,17(增刊1):2.
[19] 张国富,姚远.蜡样芽孢杆菌引起角膜炎结膜炎的诊断与分析[J].实用医技杂志,2001,8(4):252.
[20] 陈滨,胡勇平,项燕,等.蜡样芽孢杆菌致暴发性眼内炎2例[J].中国眼耳鼻喉科杂志,2009,9(4):255,274.
[21] 刘春林,徐红云,李红,等.蜡样芽孢杆菌致急性眼内炎1例[J].中国实用医药,2010,5(6):154.
[22] LEE P L,LEMOS B,O’BRIEN S H,et al.Cutaneous diphtheroid infection and review of other cutaneous gram-positive Bacillus infections[J].Cutis,2007,79(5):371-377.
[23] XING Jian-ming,ZHANG Su,DU Ying,et al.Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method[J].World Journal of Gastroenterology,2009,15(20):2537-2542.
[24] CHATTERJEE S,GHOSH T S,DAS S.Virulence of Bacillus cereus as natural facultative pathogen of Anopheles subpictus Grassi(Diptera: Culicidae)larvae in submerged rice-fields and shallow ponds[J].African Journal of Biotechnology,2010,9(41):6983-6986.
[25] ANDREA H,GUPTE A,MCAULIFFE P F,et al.Bacillus cereus necrotizing fasciitis in a patient with end-stage liver disease[J].Surgical Infections,2010,11(5):469-474.
[26] 蔡完其,黄琪琰.尼罗罗非鱼溃烂病的病理研究[J].水产学报,1986,10(3):261-271.
[27] 秦蕾.养殖大菱鲆爱德华氏菌病及其几种重要疾病的病理学研究[D].青岛:中国海洋大学,2006.
[28] 汪开毓,耿毅,陈德芳,等.斑点叉尾鮰急性流行性传染病的病原分离与病理学观察[J].中国水产科学,2007,14(3):457-465.
[29] 隗黎丽.微囊藻毒素对鱼类的毒性效应[J].生态学报,2010,30(12):3304-3310.
[30] 郭琼林,卢全章.鳗鲡爱德华氏菌病的组织病理学研究[J].水生生物学报,1995,19(1):56-60.
[31] 韩先朴,李伟,潘金培.爱德华氏菌人工经口感染及病理观察[J].水生生物学报,1995,19(3):245-249.

备注/Memo

备注/Memo:
收稿日期: 2011-12-13
通信作者: 黄惠莉(1962-),女,教授,主要从事水产免疫与防治的研究.E-mail:hlhuang@hqu.edu.cn.
基金项目: 福建省科技重点项目(2007T0010)
更新日期/Last Update: 2012-11-20