[1]王珍珠,陈国,陈宏文.罗伊乳杆菌转化甘油能力的原位再激活[J].华侨大学学报(自然科学版),2011,32(4):414-417.[doi:10.11830/ISSN.1000-5013.2011.04.0414]
　WANG Zhen-zhu,CHEN Guo,CHEN Hong-wen.In Situ Reactivation of the Ability of Lactobacillus reuteri Converting Glycerol[J].Journal of Huaqiao University(Natural Science),2011,32(4):414-417.[doi:10.11830/ISSN.1000-5013.2011.04.0414]
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罗伊乳杆菌转化甘油能力的原位再激活()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第32卷
期数:
2011年第4期
页码:
414-417
栏目:
出版日期:
2011-07-20

文章信息/Info

Title:
In Situ Reactivation of the Ability of Lactobacillus reuteri Converting Glycerol
文章编号:
1000-5013(2011)04-0414-04
作者:
王珍珠; 陈国; 陈宏文
华侨大学化工学院
Author(s):
WANG Zhen-zhu;  CHEN Guo;  CHEN Hong-wen
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
罗伊氏乳杆菌;  甘油脱水酶;  原位再激活;  静息细胞;  甘油
Keywords:
Lactobacillus reuteri;  Glycerol dehydratase;  in situ reactivation;  resting cell;  glycerol
分类号:
TQ920.1
DOI:
10.11830/ISSN.1000-5013.2011.04.0414
文献标志码:
A
摘要:
考察罗伊氏乳杆菌(L.reuteri)静息细胞转化甘油的重复利用,甘油脱水酶的原位再激活,以及补加培养基对再激活甘油脱水酶的变化.结果表明:菌体经二次转化后基本丧失转化能力,甘油对胞内甘油脱水酶具有强烈的抑制灭活作用; 确证胞内存在甘油脱水酶再激活体系; 不具转化甘油能力的L.reuteri可被补加的培养基在一定程度上再激活,使其能够重复利用.
Abstract:
In this paper,reusing of Lactobacillus reuteri resting cell,reactivation of Glycerol dehydratase in situ and reacting converting glycerol of Lactobacillus reuteri by adding fresh culture medium were studied.The results indicated that the Lactobacillus reuteri would lose its transformation ability after converting twice,glycerol had strong inhibitory activity to Glycerol dehydratase inside the cell and it was confirmed the cell owned a reactivating system for Glycerol dehydratase,and Lactobacillus reuteri which wasn′t able to convert glycerol could react to a certain extent by adding culture medium,and it could convert glycerol again.

参考文献/References:

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[5] KAJIURA H, MORI K, TOBIMATUSU T. Characterization and mechanism of action of a reactivating factor for adenosylcobalamin-dependent glycerol dehydratase [J]. the Journal of Biological Chemistry, 2001, (39):36514-36519.doi:10.1074/jbc.M105182200.
[6] 平丽英, 柳志强, 薛亚平. 罗伊乳酸杆菌甘油脱水酶基因的克隆及其在大肠杆菌中的表达 [J]. 生物工程学报, 2009, (12):1983-1988.
[7] L(U)TJI-PENG Q, SCHARER S, PUHAN Z. Production and stability of 3-hydroxypropionaLdehyde in Lactobacillus reuteri [J]. Applied Microbiology and Biotechnology, 2002, (1/2):73-80.
[8] TORAYA T, KUNO S, FUKUI S. Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in Enterobacteriaceae and Propionibacteriaceae [J]. Journal of Bacteriology, 1980(3):1439-1442.
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相似文献/References:

[1]杨仲丽,方柏山,余劲聪,等.辅酶依赖型和不依赖型甘油脱水酶的比较[J].华侨大学学报(自然科学版),2010,31(5):542.[doi:10.11830/ISSN.1000-5013.2010.05.0542]
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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20906035); 福建省自然科学基金资助项目(E0810017)
更新日期/Last Update: 2014-03-23