[1]刘青,甘林火,邓爱华,等.新氨基酸螯合钙制剂L-亮氨酸钙的急性毒性及致突变性[J].华侨大学学报(自然科学版),2009,30(4):429-431.[doi:10.11830/ISSN.1000-5013.2009.04.0429]
　LIU Qing,GAN Lin-huo,DENG Ai-hua,et al.Experimental Study on Acute Toxicity and Mutagenicity of L-Leucine Calcium of a New Amino Acid Chelate Calcium[J].Journal of Huaqiao University(Natural Science),2009,30(4):429-431.[doi:10.11830/ISSN.1000-5013.2009.04.0429]
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新氨基酸螯合钙制剂L-亮氨酸钙的急性毒性及致突变性()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第4期
页码:
429-431
栏目:
出版日期:
2009-07-20

文章信息/Info

Title:
Experimental Study on Acute Toxicity and Mutagenicity of L-Leucine Calcium of a New Amino Acid Chelate Calcium
文章编号:
1000-5013(2009)04-0429-03
作者:
刘青; 甘林火; 邓爱华; 翁连进
华侨大学化工学院
Author(s):
LIU Qing;  GAN Lin-huo;  DENG Ai-hua;  WENG Lian-jin
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
L-亮氨酸钙;  急性毒性;  染色体畸变试验;  微核试验;  精子畸形试验
Keywords:
L-leucine calcium;  acute toxicity;  chromosome shape abnormality test;  micronu-cleus test;  sperm shape deformity test
分类号:
R96
DOI:
10.11830/ISSN.1000-5013.2009.04.0429
文献标志码:
A
摘要:
研究自制的新型氨基酸螯合钙制剂——L-亮氨酸钙的急性毒性及致突变性,并对其安全性进行评价.对小鼠分别给予不同质量的L-亮氨酸钙混悬液灌胃,连续7 d观察小鼠急性毒性.将50只小鼠随机分为5组:阴性对照组、质量比为0.04 g.kg-1的环磷酰胺组,以及质量比分别为0.5,2.5,5.0 g.kg-1的L-亮氨酸钙混悬液组.通过染色体畸变试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验,观察L-亮氨酸钙的遗传毒性.结果表明,为小鼠灌胃7 d后半数致死计量(LD50)大于10 g.kg-1,L-亮氨酸钙属实际无毒物质.在染色体畸变试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验中均呈阴性反应,未显示致突变作用,表明L-亮氨酸钙无毒性和无致突变性.
Abstract:
Studies were carried out on the acute toxicity and mutagenicity of as-prepared L-leucine calcium,and its safety was evaluated.The different density L-leucine calcium solution was gived by the stomach,the acute toxicity tests was observed in seven days continuously.Fifty mice were divied into five groups randomly: the negtive contrst group,Cyclophosphamide group(0.04 g·kg-1),L-leucine calcium solution group(0.5,2.5,5.0 g·kg-1),and mutagenicity tests were evaluated through chromosome shape abnormality test,micronu-cleus test of bone marrow cell and sperm shape abnormality test in mice.LD50 was higher than 10 g·kg-1,indicating that L-leucine calcium was non-toxic,and no significant defferent was found between each test group and the control group in mutagenicity tests.These results indicate the L-leucine calcium has non-toxic and can’t induce mutation.

参考文献/References:

[1] 段惠敏, 李淑芳, 郭光美. 补钙与钙营养强化剂 [J]. 食品科技, 2002(1):64-65.doi:10.3969/j.issn.1005-9989.2002.01.027.
[2] 张经坤, 张泽民, 于傲. 人体钙吸收理论探讨 [J]. 科学通报, 2000(1):1114-1120.doi:10.3321/j.issn:0023-074X.2000.10.022.
[3] 王立升. 天门冬氨酸钙和葡萄糖酸钙兔体内吸收度的研究 [J]. 广西大学学报（自然科学版）, 2000(4):270-281.doi:10.3969/j.issn.1001-7445.2000.04.005.
[4] 张丽萍, 赵红杰, 王岑. 络合钙在小鼠体内的药代动力学研究 [J]. 中国生化药物杂志, 2000(2):71-72.doi:10.3969/j.issn.1005-1678.2000.02.006.
[5] 中华人民共和国卫生部. GB 15193.1-2003.食品安全性毒理学评价程序和方法 [S]. 北京:中国标准出版社, 2003.
[6] 黄幸纾, 陈星若. 环境化学物致突变、致畸、致癌试验方法 [M]. 杭州:浙江科学技术出版社, 1985.
[7] 张丽萍, 许国, 姚杰. 络合物用于补钙的研究 [J]. 贵州师范大学学报(自然科学版), 2000(2):28-29.

相似文献/References:

[1]刘青,甘林火,邓爱华,等.L-亮氨酸钙对小鼠股骨生长的影响[J].华侨大学学报(自然科学版),2011,32(2):191.[doi:10.11830/ISSN.1000-5013.2011.02.0191]
　LIU Qing,GAN Lin-huo,DENG Ai-hua,et al.Effect of L-Leucine Calcium on the Thighbone Growth of Mice[J].Journal of Huaqiao University(Natural Science),2011,32(4):191.[doi:10.11830/ISSN.1000-5013.2011.02.0191]

备注/Memo

备注/Memo:
泉州市科技计划重点项目(2005G6); 华侨大学科研基金资助项目(05BS301)
更新日期/Last Update: 2014-03-23