[1]郑晨娜,方柏山,罗菊香,等.链霉菌G-HD-4产黑色素的提取及理化性质[J].华侨大学学报(自然科学版),2009,30(3):292-296.[doi:10.11830/ISSN.1000-5013.2009.03.0292]
　ZHENG Chen-na,FANG Bai-shan,LUO Ju-xiang,et al.Extraction and Physico-Chemical Proterties of the Melanin Produced by Streptomyces G-HD-4[J].Journal of Huaqiao University(Natural Science),2009,30(3):292-296.[doi:10.11830/ISSN.1000-5013.2009.03.0292]
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链霉菌G-HD-4产黑色素的提取及理化性质()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第3期
页码:
292-296
栏目:
出版日期:
2009-05-20

文章信息/Info

Title:
Extraction and Physico-Chemical Proterties of the Melanin Produced by Streptomyces G-HD-4
文章编号:
1000-5013(2009)03-0292-05
作者:
郑晨娜; 方柏山; 罗菊香; 靳鸿蔚
华侨大学工业生物技术福建省高等学校重点实验室
Author(s):
ZHENG Chen-na;  FANG Bai-shan;  LUO Ju-xiang;  JIN Hong-wei
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
链霉菌G-HD-4;  黑色素;  提取;  纯化;  理化性质
Keywords:
Streptomyces G-HD-4;  melanin;  extraction;  purification;  physical-chemical properties
分类号:
TS264.4
DOI:
10.11830/ISSN.1000-5013.2009.03.0292
文献标志码:
A
摘要:
对链霉菌G-HD-4发酵液采用酸沉淀、碱性溶液提取、酸水解,以及多种有机溶剂抽提等工艺手段分离、纯化出黑色素,研究其理化性质.结果表明,该黑色素的最大吸收峰为246 nm,与标准黑色素最大吸收峰相一致.在400 nm波长下,其光密度值(D)随黑色素质量浓度的增加而增大; 容易被强氧化剂所氧化,而紫外、光照、温度、有机溶剂、还原剂、各种盐,以及金属离子对黑色素的影响较小,氨基酸、淀粉等添加物对黑色素稳定性没有影响,说明该黑色素稳定性良好.
Abstract:
The melanin derived from ferment broth of Streptomyces G-HD-4 was isolated and purified by acid deposition,alkaline extraction,acid hydrobysis and multiplicate organic solvents extraction,and their physical and chemical properties were studied.The result shows that the maximum absorption peak of the melanin was 246 nm which agree with the typical melanin.The absorbance of the melanin increase with an increase of melanin’s concentration at 400 nm.The melanin could be bleached by strong oxidants,but it had less sensitivity to ultraviolet light or room light,temperature,organic solvents,reducing agents,salts and metal-ions for the melanin.Different of additions such as amino acid,starch et al,had no effect on the stability of melanin.Physical and chemical properties of the melanin revealed that the melanin from Streptomyces G-HD-4 had a favorable stability.

参考文献/References:

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[1]陈世年.出芽短梗霉发酵生产卜多糖的研究　(I)不产黑色素酵母样形态菌株的选育[J].华侨大学学报(自然科学版),1995,16(2):223.[doi:10.11830/ISSN.1000-5013.1995.02.0223]
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备注/Memo

备注/Memo:
国家重点基础研究发展(973)计划项目(2007CB707804); 国家高技术研究发展(863)计划项目(2006AA020103); 国家自然科学基金资助项目(30770059); 国务院侨办科研基金资助项目(07QZR07)
更新日期/Last Update: 2014-03-23