[1]蔡福营,樊云,张光亚,等.杀虫晶体蛋白对小菜蛾杀虫活性的识别预测[J].华侨大学学报(自然科学版),2009,30(1):53-55.[doi:10.11830/ISSN.1000-5013.2009.01.0053]
　CAI Fu-ying,FAN Yun,ZHANG Guang-ya,et al.The Discrimination Prediction with Insecticidal Activity of Insecticidal Crystal Protein Against Plutella xylostella[J].Journal of Huaqiao University(Natural Science),2009,30(1):53-55.[doi:10.11830/ISSN.1000-5013.2009.01.0053]
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杀虫晶体蛋白对小菜蛾杀虫活性的识别预测()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第1期
页码:
53-55
栏目:
出版日期:
2009-01-20

文章信息/Info

Title:
The Discrimination Prediction with Insecticidal Activity of Insecticidal Crystal Protein Against Plutella xylostella
文章编号:
1000-5013(2009)01-0053-03
作者:
蔡福营; 樊云; 张光亚; 林毅
华侨大学化工学院
Author(s):
CAI Fu-ying;  FAN Yun;  ZHANG Guang-ya;  LIN Yi
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
苏云金芽胞杆菌;  杀虫晶体蛋白;  二肽组成;  杀小菜蛾活性;  识别预测
Keywords:
Bacillus thuringiensis;  insecticidal crystal proteins;  dipeptide composition;  activity against Plutella xylostella;  discrimination
分类号:
S476
DOI:
10.11830/ISSN.1000-5013.2009.01.0053
文献标志码:
A
摘要:
考察对小菜蛾具有杀虫活性和无杀虫活性的晶体蛋白二肽组成的差异.结果发现,对小菜蛾具有杀虫活性的晶体蛋白中,SG,LS,SN,NI,GS,SV,SL,TV,EF,NQ二肽分数较高; 而对小菜蛾无杀虫活性的晶体蛋白中,AN,LQ,DA,AQ,TF,SY,EI,LE,PT,RA二肽分数较高,总体分数上存在差异最大的是S*型二肽.在此基础上,发展一种识别杀虫晶体蛋白(ICP)对小菜蛾杀虫活性的统计学方法,通过对两组共27条杀虫晶体蛋白进行有无杀虫活性的初步识别,该方法识别平均正确率分别是71.4%和100%.
Abstract:
In this work,the dipeptide composition of crystal proteins with and without insecticidal activity against Plutella xylostella was systematically analyzed.We found that crystal proteins with insecticidal activity against Plutella xylostella contained more dipeptides such as SG,LS,SN,NI,GS,SV,SL,TV,EF and NQ,whereas less dipeptides such as AN,LQ,DA,AQ,TF,SY,EI,LE,PT and RA,and generally,S* was the most different dipeptide.Based on this information,a statistical method for discriminating 27 crystal proteins with or without insecticidal activity against Plutella xylostella was developed and their accuracy was 71.4% and 100%,respectively.

参考文献/References:

[1] HOFTE H, WHITELEY H. Insecticidal crystal proteins of Bacillus thuringiensis [J]. Microbiological Reviews, 1989, (36):242-255.
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[4] 张光亚, 方柏山. 基与二肽组成识别嗜热和常温蛋白的研究 [J]. 生物工程学报, 2006(2):293-298.doi:10.3321/j.issn:1000-3061.2006.02.021.
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[7] 林毅, 蔡福营, 张光亚. 苏云金杆菌杀虫晶体蛋白活性预测的支持向量机模型 [J]. 生物工程学报, 2007(1):127-132.doi:10.3321/j.issn:1000-3061.2007.01.022.

相似文献/References:

[1]洪雪梅,林毅.新型杀虫晶体蛋白Cry7Ab4空间结构的计算机解析[J].华侨大学学报(自然科学版),2012,33(1):60.[doi:10.11830/ISSN.1000-5013.2012.01.0060]
　HONG Xue-mei,LIN Yi.Structural Analysis of a Novel Insecticidal Crystal Protein Cry7Ab4[J].Journal of Huaqiao University(Natural Science),2012,33(1):60.[doi:10.11830/ISSN.1000-5013.2012.01.0060]

备注/Memo

备注/Memo:
国家自然科学基金资助项目(40601046); 福建省自然科学基金资助项目(B0510011); 福建省高等学校新世纪优秀人才计划支持项目(2006)
更新日期/Last Update: 2014-03-23