[1]王昭晶,罗巅辉.穿山龙多糖的提取与纯化工艺条件优化[J].华侨大学学报(自然科学版),2007,28(3):296-299.[doi:10.3969/j.issn.1000-5013.2007.03.019]
　WANG Zhao-jing,LUO Dian-hui.The Isolation,Separation,Purification and Property Research of Polysaccharide Extracted from Dioscorea nipponica Makino[J].Journal of Huaqiao University(Natural Science),2007,28(3):296-299.[doi:10.3969/j.issn.1000-5013.2007.03.019]
点击复制

穿山龙多糖的提取与纯化工艺条件优化()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第28卷
期数:
2007年第3期
页码:
296-299
栏目:
出版日期:
2007-07-20

文章信息/Info

Title:
The Isolation,Separation,Purification and Property Research of Polysaccharide Extracted from Dioscorea nipponica Makino
文章编号:
1000-5013(2007)03-0296-04
作者:
王昭晶; 罗巅辉
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021
Author(s):
WANG Zhao-jing;  LUO Dian-hui
College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
多糖;  穿山龙;  浸提;  纯化
Keywords:
polysaccharide;  Dioscorea nipponica Makino;  extration;  purification
分类号:
S823
DOI:
10.3969/j.issn.1000-5013.2007.03.019
文献标志码:
A
摘要:
通过单因素法,研究从穿山龙中提取水溶性多糖的工艺条件,以及分析原料质量与提取液体积的比(料液比)、浸提温度和提取时间3个主要因素对提取量的影响,并对热水浸提穿山龙多糖的工艺条件进行优化.实验表明,水提多糖最佳提取工艺条件:料液比(g∶mL)为1∶4,浸提温度为90℃,浸提时间为2.5 h.按最佳工艺条件水煮提,醇沉干燥得到粗多糖(CP),经柱层析分离纯化后,可得组分CP-Ⅰ,CP-Ⅱ.采用柱层析、纸电泳检测组分的纯度,并利用高效液相色谱法和纸层析法分析组成,结果表明,CP-Ⅱ为单一多糖,由鼠李糖(Rha)、果糖(Fru)和葡萄糖(Glu)3种单糖组成,其量比n(Rha)∶n(Fru)∶n(Glu)为1∶1.08∶48.6.
Abstract:
This paper studies the process of extracting polysaccharides from Dioscorea nipponica Makino.During the extracting process,three main factors including ratio of solid to liquid,exacting time and temperature were studied by using single factor analysis method.On basis of above,the optimal process conditions of hot water extraction of polysaccharides were obtained by orthogonal as follows: ratio of solid to liquid 1∶4(g∶mL),extracting duration 2.5 hours,and extracting temperature 90 ℃.A water-soluble crude polysaccharide(CP) was obtained after extracting with the optimal extracting conditions.Coarse amylose had to be purified,and two water-soluble polysaccharides named CP-Ⅰ and CP-Ⅱ were obtained.It was indicated that CP-Ⅱ was homogeneous by gel filtration chromatography and cellulose acetate pellicle electrophoresis.The monosaccharides of CP-Ⅱ were analyzed by using high performance liquid chromatography(HPLC) and paper chromatography(PC).The result showed that CP-Ⅱ was mainly composed of Rha,Fru and Glu,in the molar ratio of 1∶1.08∶48.6.

参考文献/References:

[1] 王岳宝. 福建省中药炮制规范 [M]. 福州:福建科学出版社, 1988.330.
[2] 郑永进, 郑爱群. 穿山龙与南蛇藤根的鉴别 [J]. 实用中医药杂志, 2002, (11):53.doi:10.3969/j.issn.1004-2814.2002.11.067.
[3] 刘江涛, 陈信义, 王玉芝. 穿山龙粗提物抗肿瘤体外实验研究 [J]. 中国中医药信息杂志, 2002(3):206-207.doi:10.3969/j.issn.1005-5304.2004.03.009.
[4] 张惟杰. 复合多糖生化研究技术 [M]. 上海:上海科学技术出版社, 1987.6-7.
[5] WANG Zhao-jing, LUO Dian-hui, LIANG Zhong-yuan. Structure of polysaccharides from the fruiting body of Hericium erinaceus Pers [J]. Carbohydrate Polymers, 2004, (57):241-247.
[6] 王昭晶, 梁忠岩, 罗巅辉. 小刺猴头菌子实体水溶性多糖HPI的结构研究 [J]. 高等学校化学学报, 2004, (8):1474-1476.doi:10.3321/j.issn:0251-0790.2004.08.049.

备注/Memo

备注/Memo:
华侨大学科研基金资助项目(04HZR03)
更新日期/Last Update: 2014-03-23