[1]夏启容,方柏山,洪燕.酶体外定向进化(Ⅲ)展示技术及其应用[J].华侨大学学报(自然科学版),2005,26(4):333-337.[doi:10.3969/j.issn.1000-5013.2005.04.001]
　Xia Qirong,Fang Baishan,Hong Yan.Directed Evolution of Enzymes in Vitro(Ⅲ) Display Technology and Its Application[J].Journal of Huaqiao University(Natural Science),2005,26(4):333-337.[doi:10.3969/j.issn.1000-5013.2005.04.001]
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酶体外定向进化(Ⅲ)展示技术及其应用()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第26卷
期数:
2005年第4期
页码:
333-337
栏目:
出版日期:
2005-10-20

文章信息/Info

Title:
Directed Evolution of Enzymes in Vitro(Ⅲ) Display Technology and Its Application
文章编号:
1000-5013(2005)04-0333-05
作者:
夏启容; 方柏山; 洪燕
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021
Author(s):
Xia Qirong;  Fang Baishan;  Hong Yan
College of Material Science and Engineering, Huaqiao University, 362021, Quanzhou, China
关键词:
展示技术;  酶;  定向进化;  噬菌体;  细胞表面;  核糖体;  mRNA
Keywords:
display technology;  enzyme;  directed evolution;  phage;  cell surface;  ribosome;  mRNA
分类号:
Q78
DOI:
10.3969/j.issn.1000-5013.2005.04.001
文献标志码:
A
摘要:
利用展示技术构建的蛋白质/多肽的突变体库,可以通过高通量进行高效地分析和筛选,因此特别适用于改造蛋白质.文中综述噬菌体、细胞表面、核糖体、mRNA等展示技术的原理和方法,以及其在酶定向进化中的应用.
Abstract:
With the ability of conveniently assaying and analyzing a large number of mutated proteins in a high-throughput way,display systems of libraries of mutated proteins or peptides on phage or some biological resources have been used to improve proteins and enzymes.In this article,we review recent studies on display technology,which include not only the principles and methods of phage display,cell-surface display,ribosome display and mRNA display,but also their application in aspect of directed evolution of enzymes.

相似文献/References:

[1]冯真泰,颜文礼.辐射聚合在酶的固定化中应用概述[J].华侨大学学报(自然科学版),1991,12(3):331.[doi:10.11830/ISSN.1000-5013.1991.03.0331]
　Feng Zhentai,Yan Wenli.Application of Radiopolymerization to the Immobilization of Enzymes[J].Journal of Huaqiao University(Natural Science),1991,12(4):331.[doi:10.11830/ISSN.1000-5013.1991.03.0331]

备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026,20446004); 福建省科技计划重点基金资助项目(2003I020)
更新日期/Last Update: 2014-03-23