[1]张金梅,杜予民.水溶性有机介质中漆树酶催化氧化对茴香胺[J].华侨大学学报(自然科学版),2004,25(4):446-448.[doi:10.3969/j.issn.1000-5013.2004.04.026]
　Zhang Jinmei,Du Yumin.Catalytic Oxidation of P-Anisidine with Rhus Laccase in Water Soluble Organic Solvents[J].Journal of Huaqiao University(Natural Science),2004,25(4):446-448.[doi:10.3969/j.issn.1000-5013.2004.04.026]
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水溶性有机介质中漆树酶催化氧化对茴香胺()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第25卷
期数:
2004年第4期
页码:
446-448
栏目:
出版日期:
2004-10-20

文章信息/Info

Title:
Catalytic Oxidation of P-Anisidine with Rhus Laccase in Water Soluble Organic Solvents
文章编号:
1000-5013(2004)04-0446-03
作者:
张金梅; 杜予民
暨南大学化学系; 武汉大学环境科学系 广东广州510632; 湖北武汉430072
Author(s):
Zhang Jinmei1;  Du Yumin2
1.Dept. of Chem., Jinan Univ., 510632, Guangzhou, China; 2.Dept. of Environ. Sci., Wuhan Univ., 430072, Wuhan, China
关键词:
漆树酶;  有机介质;  对茴香胺;  催化氧化
Keywords:
Rhus laccase;  organic media;  p-anisidine;  catalytic oxidation
分类号:
O643.3
DOI:
10.3969/j.issn.1000-5013.2004.04.026
文献标志码:
A
摘要:
采用分光光度法,考察不同水 /水溶性有机溶剂体系中漆树酶的催化氧化性能,以及影响反应的因素 .研究结果表明,漆树酶在醇溶剂中的活性要强于在非醇溶剂中,醇分子中羟基数量对漆树酶活性也有影响 .在温度 4 5℃,pH值为 7.5,体积比为 1∶1的水 /丙三醇溶液中,漆树酶的活力最高,活力比达 15 0 %以上,超出漆树酶在纯水中的活性 .以漆树酶催化氧化对茴香胺,采用TLC和HPLC分离纯化反应产物 根据光谱分析法,鉴定主要产物为偶氮茴香醚
Abstract:
By adopting spectrophotometry, the authors inspected the performance of Rhus laccase catalytic oxidation in differenl water/water soluble organic solvent systems; and the factors influencing the reaction. It was found that Rhus laccase shows better activity in alcohols than in nonalcoholic organic solvent; and the number of hydroxyl groups in alcohol molecule also exerts influence on the activity of Rhus laccase. In water/glycerin solution where volume ratio of water and glycerin was 1∶1 and temperature was around 45 ℃ and pH was around 7.5, Rhus laccase showed the highest activity, which was for better than that in pure water, with an activity ratio over 150%. Then the p-anisidine was catalytically oxidized with Rhus laccase as catalyst, the reaction product was separated and purified by HPLC and TLC. According to spectrophotometry, the main product was determined to be p-azoanisol.

参考文献/References:

[1] 王习文, 詹怀宇. 漆酶/介体系统生物漂白技术 [J]. 西南造纸, 2002(3):7-10.
[2] 季立才, 胡培植. 漆酶催化氧化研究进展 [J]. 林产化学与工业, 1997(1):79-84.
[3] Mayer A M, Staples R C. Laccase: New functions for an old enzyme [J]. Phytochemistry, 2002.551-565.doi:10.1016/S0031-9422(02)00171-1.
[4] Rodakiewicz-Nowak J, Kasture S M, Dudek B. Effect of various water-miscible solvents on enzymatic activity of fungal laccases [J]. Journal of Molecular Catalysis B:Enzymatic, 2000, (11):1-7.doi:10.1016/S1381-1177(00)00183-1.
[5] 詹东风, 杜予民, 钱保功. 醇水介质中漆酶催化氧化漆酚及其类似物 [J]. 高分子学报, 1990(3):327-331.
[6] 杜予民, 张金梅. 反胶团中漆树酶催化氧化性能与反应产物鉴定 [J]. 高等学校化学学报, 1995(5):715-718.
[7] Reinhammar B. Purification and properties of laccase and stellacyanin from Rhus vernicif era [J]. Biochimica et Biophysica Acta, 1970(1):35-49.doi:10.1016/0005-2728(70)90059-9.
[8] 何为, 詹怀宇, 王习文. 一种改进的漆酶酶活检测检测方法 [J]. 华南理工大学学报(自然科学版), 2003, (12):46-50.doi:10.3321/j.issn:1000-565X.2003.12.012.
[9] 伊凡海耳, 中国科学院《自然科学名词》编订室. 汉译海氏有机化合物词典 [M]. 北京:科学出版社, 1965.318-319.

更新日期/Last Update: 2014-03-23