[1]张光亚,方柏山,闵航,等.一株好氧反硝化菌的特征及系统进化分析[J].华侨大学学报(自然科学版),2004,25(1):75-78.[doi:10.3969/j.issn.1000-5013.2004.01.019]
　Zhang Guangya,Fang Baishan,Min Hang,et al.Characteristics and Phylogenetic Analysis of a Strain of Aerobic Denitrifier[J].Journal of Huaqiao University(Natural Science),2004,25(1):75-78.[doi:10.3969/j.issn.1000-5013.2004.01.019]
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一株好氧反硝化菌的特征及系统进化分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第25卷
期数:
2004年第1期
页码:
75-78
栏目:
出版日期:
2004-01-20

文章信息/Info

Title:
Characteristics and Phylogenetic Analysis of a Strain of Aerobic Denitrifier
文章编号:
1000-5013(2004)01-0075-04
作者:
张光亚; 方柏山; 闵航; 陈美慈
华侨大学材料科学与工程学院; 浙江大学生命科学学院; 浙江大学生命科学学院 福建泉州362011; 福建泉州362011; 浙江杭州310029; 浙江杭州310029
Author(s):
Zhang Guangya1;  Fang Baishan1;  Min Hang2;  Chen Meici2
1 College of Mater. Sci. & Eng., Huaqiao Univ, 362011, Quanzhou, China; 2.College of Life science, Zhejiang Univ., 310029, Hangzhou, China
关键词:
好氧反硝化菌;  Rhodococcussp.HN;  16SrDNA序列;  系统进化分析
Keywords:
aerobic denitrifying bacteria;  Rhodococcus sp. HN;  16S rDNA sequence;  phylogenetic analysis
分类号:
X703
DOI:
10.3969/j.issn.1000-5013.2004.01.019
文献标志码:
A
摘要:
从土壤中分离出一株好氧反硝化菌,命名为菌株HN,分离菌株呈革兰氏染色阳性,为球状或杆状,菌落颜色显橙红色 .该菌株以硝酸钠为氮源时,进行好氧反硝化作用,能以乙酰胺为唯一碳源和氮源进行生长 .它的部分长度 1 6SrDNA序列分析表明,分离菌株HN与Rhodococcusruber的 1 6SrDNA序列具有 99%相似性 .实验采用PHYLIP程序对该菌株与报道菌进行系统发育进化分析
Abstract:
A strain of aerobic denitrifying bacteria was isolated from the soil and named as strain HN. They are bacteria of gram positive, spherical or rod like; they often become orang red colony. They can carry out denitrification when sodium nitrate is taken as nitrogen source; and they can grow with acetamide as unique carbon source and nitrogen source. As indicated by analysing 16S rDNA sequence of partial length, this isolated strain bear similarity to 16S rDNA sequence of Rhodococcas ruber . By applying PHYLIP program, phylogenetic analysis was made on strain HN and the relative strains.

参考文献/References:

[1] 丁爱中, 傅家谟, 盛国英. 好氧生物反硝化反应的实验证据 [J]. 科学通报, 2000, (z1):2779-2782.doi:10.3321/j.issn:0023-074X.2000.Z1.016.
[2] 冯叶成, 王建龙, 钱易. 同时硝化反硝化的试验研究 [J]. 上海环境科学, 2002(9):527-529.
[3] Lukow T, Diekmann H. Aerobic denitrification by a newly isolated heterotrophic bacterium strain TL1 [J]. Biotechnology Letters, 1997, (19):1157-1159.
[4] Huang H K, Tseng S K. Nitrate reduction by Citrobacter diversus under aerobic environment [J]. Applied Microbiology and Biotechnology, 2001, (55):90-94.
[5] Sneath P H A, Mair N S, Sharpe M E. Bergey’s Manual of Systematic Bacteriology [M]. Lippincott: Milliams Wilkins, 1986.2366-2367.
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备注/Memo

备注/Memo:
浙江省自然科学基金资助项目(399214); 华侨大学科研基金资助项目(03HZR7)
更新日期/Last Update: 2014-03-23