[1]肖美添,李锋,唐凤翔,等.麒麟菜粗多糖中多糖与蛋白质结合方式[J].华侨大学学报(自然科学版),2003,24(3):295-299.[doi:10.3969/j.issn.1000-5013.2003.03.014]
　Xiao Meitian,Li Feng,Tang Fengxiang,et al.The Mode of Binding Polysaccharide and Protein Together in Crude Polysaccharide of Eucheume Cottoni[J].Journal of Huaqiao University(Natural Science),2003,24(3):295-299.[doi:10.3969/j.issn.1000-5013.2003.03.014]
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麒麟菜粗多糖中多糖与蛋白质结合方式()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第24卷
期数:
2003年第3期
页码:
295-299
栏目:
出版日期:
2003-07-20

文章信息/Info

Title:
The Mode of Binding Polysaccharide and Protein Together in Crude Polysaccharide of Eucheume Cottoni
文章编号:
1000-5013(2003)03-0295-05
作者:
肖美添; 李锋; 唐凤翔; 郭养浩
华侨大学材料科学与工程学院; 福州大学药物生物技术与工程研究所; 福州大学药物生物技术与工程研究所 福建泉州362011; 福建福州350002; 福建福州350002
Author(s):
Xiao Meitian1;  Li Feng2;  Tang Fengxiang2;  Guo Yanghao2
1.College of Mater. Sci. & Eng., Huaqiao Univ., 362011, Quanzhou, China; 2.Inst, of Pharma. Biotech. & Eng., Fuzhou Univ., 350001, Fuzhou, China
关键词:
麒麟菜;  粗多糖;  蛋白质分离;  结合方式
Keywords:
Eucheume cottoni;  crude polysaccharide;  separation of protein;  the mode of binding
分类号:
O636.1
DOI:
10.3969/j.issn.1000-5013.2003.03.014
文献标志码:
A
摘要:
从海藻麒麟菜中提取的粗多糖含有一定量的蛋白质,采用 Sevag法在除麒麟菜粗多糖蛋白质,则蛋白质与多糖以相同的比例减少 .粗多糖酸解液的 p H调整为 1 1 .0后,经凝胶 Sephadex G-1 0 0层析,能使蛋白质部分分离,而酸性条件无法使之分离 .实验结果表明,粗多糖中蛋白质不是以游离的形式存在,可能以离子键方式与多糖分子中的硫酸根聚阴离子相结合 .提取麒麟菜多糖和纯化过程中,控制合适的 p H条件有利于分离蛋白质 .
Abstract:
Certain amount of proteins are contained in the crude polysaccharide extracting from the seaweed Eucheume cottoni . In removing proteins from crude polgasccharide of Eucheume cottoni by adopting Sevag method, both proteins and polysaccharide reduce in same proportion. The crude polysaccharide liquid of acidolysis is passed through gel Sephadex G100 chromatography after the value its pH is adjusted to 11.0, partial separation of proteins can be occured but separation of protein can not be occurred in acid medium. As shown by experimental result, protein does not exist freely in crude polysaccharide; proteins may bind in the form of ionic bonds to polyanion of sulfate radicals in polysaccharide molecule. During extracting and purifying polysaccharide from Eucheume cotloni, controlling suitable pH condition is advantageous to the separation of protein.

参考文献/References:

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备注/Memo

备注/Memo:
福建省教育厅科研基金资助项目(K02014)
更新日期/Last Update: 2014-03-23