[1]戴四发,贺淹才.黑曲霉产纤维素酶系各组分特性及酶解条件[J].华侨大学学报(自然科学版),2001,22(1):65-69.[doi:10.3969/j.issn.1000-5013.2001.01.015]
　Dai Sifa,He Yancai.Characteristic of Respective Components from Cellulase System Induced by Aspergillus Niger and Condition of Enzymolysis[J].Journal of Huaqiao University(Natural Science),2001,22(1):65-69.[doi:10.3969/j.issn.1000-5013.2001.01.015]
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黑曲霉产纤维素酶系各组分特性及酶解条件()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第22卷
期数:
2001年第1期
页码:
65-69
栏目:
出版日期:
2001-01-20

文章信息/Info

Title:
Characteristic of Respective Components from Cellulase System Induced by Aspergillus Niger and Condition of Enzymolysis
文章编号:
1000-5013(2001)01-0065-05
作者:
戴四发; 贺淹才
华侨大学材料科学与工程学院, 泉州362011
Author(s):
Dai Sifa;  He Yancai
College of Mater. Sci. & Eng., Huaqiao Univ., 362011, Quanzhou
关键词:
黑曲霉;  纤维素酶系;  酶组分;  液体发酵;  酶解条件
Keywords:
Aspergillus Niger;  cellulase system;  enzyme component;  liquid fermentation;  enzymolysis condition
分类号:
Q556.03
DOI:
10.3969/j.issn.1000-5013.2001.01.015
摘要:
采用正交实验法分析出黑曲霉 3.316纤维素酶系各组分最适酶解条件,其组合分别为 C1酶 (p H5.0,4 5℃ ),Cx 酶 (p H3.5,6 5℃ )和 βG酶 (p H4 .5,6 5℃ ) .在液体发酵条件下,添加质量分数为 0 .0 0 3的碳酸钙时,各组分酶活性最高,其中以 C1酶明显增高,Cx 酶几乎无影响,βG酶则明显降低 .C1和 Cx 比酶活只有在碳酸钙质量分数小于 0 .0 0 5时才有提高,βG酶则明显降低 .添加蛋白胨,C1和 βG酶的最高酶活在碳酸钙质量分数小于 0 .0 0 3时有增加,Cx 酶则无明显变化 .碳酸钙和蛋白胨对各组分最高酶活形成时间均无影响 .在各组分酶分泌规律方面,仅有碳酸钙对 C1和βG酶有一定影响
Abstract:
By adopting orthogonal test, the optimel enzymolyis condition for respective component of cellase system from Aspergillus Niger 3.316 induced liquid fermentation are found to be pH5.0, 45 ℃ for enzyme C 1, and pH3.5, 65 ℃ for enzyme C x and pH4.5, 65 ℃ for enzyme β G. Under the condition of liquid state fermentation, the addition of CaCO 3 in a concentration of 0.3% leads to the highest enzyme activity of varions components. In which that of C 1 obviously increases, that of C x is almost unaffected, while that of β G obviously decreases. The enzyme activity ratio of C 1 and C x increases only if CaCO 3 keeps in a concentration below 0.5%, while C x shows no significant change. The addition of peptone leads to the highest enzyme activity of C 1 and β G of CaCO 3 keeps in a concentration below 0.3% while it leads to insignibicant change of C x . The formation time of highest enzyme activity of respective lomponent are uneffected by CaCO 3 and peptone. Regarding to the rule of enzyme secretion of respective component, only CaCO 3 shows a certain effect on C 1 and β G.

参考文献/References:

[1] StenbergK, Galbe M, Zacchi G. The influence of lactic acid formation on the stimultaneous saccharifacation and fermantation of softwood to ethanol-using saccharomyces cerevisiae and beta-glucosidase and cellulase-mediated hydrolysis [J]. Enzyme and Microbial Technology, 1990(1):71-79.doi:10.1016/S0141-0229(99)00127-1.
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[3] 赵小立, 周红军, 费承伟. 黑曲霉HD9478纤维素酶固体发酵条件的研究 [J]. 粮食与饲料工业, 1997(5):21-23.
[4] 余晓斌, 李晓华, 丁建琴. 黑曲霉产纤维素酶的研究 [J]. 生物技术, 1997(4):13-15.
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备注/Memo

备注/Memo:
福建省自然科学基金资助项目
更新日期/Last Update: 2014-03-23