[1]陈碧娥.单酶法快速测定发酵液中的L-乳酸[J].华侨大学学报(自然科学版),1998,19(3):311-313.[doi:10.11830/ISSN.1000-5013.1998.03.0311]
　Chen Bie.Single Enzyme Method for Rapidly Determining L-Lactic Acid in Fermentation Liquid[J].Journal of Huaqiao University(Natural Science),1998,19(3):311-313.[doi:10.11830/ISSN.1000-5013.1998.03.0311]
点击复制

单酶法快速测定发酵液中的L-乳酸()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第19卷
期数:
1998年第3期
页码:
311-313
栏目:
出版日期:
1998-07-20

文章信息/Info

Title:
Single Enzyme Method for Rapidly Determining L-Lactic Acid in Fermentation Liquid
作者:
陈碧娥
华侨大学化工与生化工程系
Author(s):
Chen Bie
关键词:
L-乳酸;  L-乳酸脱氢酶;  测定
Keywords:
L-lactic acid;  L-lactic acid dehydrogenase;  determination
分类号:
TQ921.3
DOI:
10.11830/ISSN.1000-5013.1998.03.0311
摘要:
介绍一种简便、快速测定发酵液中L-乳酸的新方法.采用不依赖NAD的L-乳酸脱氢酶(L-LDH),以菲咯啉铁复合物(O-phenathroline-Fe3+)作为其电子受体,吸收波长510nm,在751分光光度计上直接测定发酵液中的L-乳酸.
Abstract:
A new handy method is presented for rapidly determining L lactic acid in fermentation liquid. By adopting NAD independent L lactic acid dehydrogenase, and also Fe 3+ phenanthnoline as electron acceptor, L lactic acid in fermentation liquid can be

相似文献/References:

[1]陈碧娥,李旭,李文琦.L-乳酸产生菌的诱变选育[J].华侨大学学报(自然科学版),1993,14(3):377.[doi:10.11830/ISSN.1000-5013.1993.03.0377]
　Chen Bie,Li Xu,Li Wenqi.Mutation Breeding of L-Lactic Acid Producing Strain[J].Journal of Huaqiao University(Natural Science),1993,14(3):377.[doi:10.11830/ISSN.1000-5013.1993.03.0377]
[2]陈碧娥.离子交换法提取L-乳酸新工艺的研究[J].华侨大学学报(自然科学版),1995,16(2):231.[doi:10.11830/ISSN.1000-5013.1995.02.0231]
　Chen Bie.Ion Exchange Resin Process as a New Technology for Extracting L-Lactic Acid[J].Journal of Huaqiao University(Natural Science),1995,16(3):231.[doi:10.11830/ISSN.1000-5013.1995.02.0231]

备注/Memo

备注/Memo:
福建省自然科学基金
更新日期/Last Update: 2014-03-22