[1]陈少欣,林文銮.阳离子反胶团体系提取蛋白质[J].华侨大学学报(自然科学版),1998,19(3):304-307.[doi:10.11830/ISSN.1000-5013.1998.03.0304]
 Chen Shaoxin,Lin Wenluan.Protein Extraction by Adopting Cationic Reverse Micelle[J].Journal of Huaqiao University(Natural Science),1998,19(3):304-307.[doi:10.11830/ISSN.1000-5013.1998.03.0304]
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阳离子反胶团体系提取蛋白质()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第19卷
期数:
1998年第3期
页码:
304-307
栏目:
出版日期:
1998-07-20

文章信息/Info

Title:
Protein Extraction by Adopting Cationic Reverse Micelle
作者:
陈少欣林文銮
华侨大学化工与生化工程系
Author(s):
Chen Shaoxin Lin Wenluan
关键词:
反胶团 萃取 蛋白质
Keywords:
reverse micelle extraction protein
分类号:
Q510.3,
DOI:
10.11830/ISSN.1000-5013.1998.03.0304
摘要:
用阳离子反胶团体系(CTAB/正戊醇-正辛烷)分别对4种蛋白质进行反胶团提取的试验和考察,确定pH值、离子强度、表面活性剂浓度和助溶剂等影响提取的主要因素.实验结果表明,反胶团的提取过程与蛋白质本身的性质和反胶团体系有关,且静电作用在提取中起主要作用; 利用阳离子体系提取蛋白质时,为了获得高的萃取率,应控制低盐深度和高的pH-pI值.
Abstract:
Four kinds of proteins are extracted respectively by adopting cetane trimethyl ammonia bromide(CTAB)/n amyl alcohol octyl alcohol. A study is devoted to the effects of pH value, ionic strength, concentration of surfactant and cosolvent on the extraction.

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备注/Memo

备注/Memo:
福建省自然科学版基金
更新日期/Last Update: 2014-03-22