[1]于莹,彭永刚,李明春,等.氧化透明质酸交联碳酰肼明胶/半胱氨酸壳聚糖复合水凝胶的制备及pH响应和抗氧化性能[J].华侨大学学报(自然科学版),2025,46(6):660-665.[doi:10.11830/ISSN.1000-5013.202510012]
　YU Ying,PENG Yonggang,LI Mingchun,et al.Preparation of Oxidized Hyaluronic Acid Crosslinked Carbohydrazide-Gelatin/Cysteine-Chitosan Composite Hydrogel With pH-Responsive and Antioxidant Properties[J].Journal of Huaqiao University(Natural Science),2025,46(6):660-665.[doi:10.11830/ISSN.1000-5013.202510012]
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氧化透明质酸交联碳酰肼明胶/半胱氨酸壳聚糖复合水凝胶的制备及pH响应和抗氧化性能()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第46卷
期数:
2025年第6期
页码:
660-665
栏目:
出版日期:
2025-11-20

文章信息/Info

Title:
Preparation of Oxidized Hyaluronic Acid Crosslinked Carbohydrazide-Gelatin/Cysteine-Chitosan Composite Hydrogel With pH-Responsive and Antioxidant Properties
文章编号:
1000-5013(2025)06-0660-06
作者:
于莹1; 2;  彭永刚1; 2;  李明春1; 2;  辛梅华1; 2
1. 华侨大学 材料科学与工程学院, 福建 厦门 361021;2. 华侨大学 环境友好功能材料教育部工程中心, 福建 厦门 361021
Author(s):
YU Ying1; 2;  PENG Yonggang1; 2;  LI Mingchun1; 2;  XIN Meihua1; 2
1. College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China; 2. Engineering Research Center of Environment-Friendly Functional Materials, Ministry of Education, Huaqiao University, Xiamen 361021, China
关键词:
水凝胶;  壳聚糖;  pH响应;  抗氧化
Keywords:
hydrogel;  chitosan;  pH-responsive;  antioxidant
分类号:
O636.1
DOI:
10.11830/ISSN.1000-5013.202510012
文献标志码:
A
摘要:
为提升天然高分子水凝胶的生物功能,设计并构建一种基于氧化透明质酸(OHA)、半胱氨酸接枝壳聚糖(CSCys)与碳酰肼改性明胶(Gel-CDH)的pH响应型复合水凝胶。该水凝胶通过醛基与氨基/肼基之间的席夫碱反应实现动态共价交联,形成稳定且可调控的三维网络结构。通过调节OHA比例,制得3种水凝胶,并系统评估其溶胀行为与pH值的关系及抗氧化活性。结果表明:该复合水凝胶不仅具备良好的pH响应性,还因巯基等功能团的协同作用,展现出优异的自由基清除能力。
Abstract:
To enhance the biological functionality of natural polymer hydrogels, a pH-responsive composite hydrogel was designed and constructed based on oxidized hyaluronic acid(OHA), cysteine-grafted chitosan(CSCys), and carbonyl hydrazide-modified gelatin(Gel-CDH). The hydrogel achieved dynamic covalent crosslinking through Schiff base reactions between aldehyde groups and amino/hydrazide groups to form a stable and tunable three-dimensional network structure. By adjusting the OHA ratio, three types of hydrogels were prepared, and their swelling behavior in relation to pH values as well as antioxidant activity were systematically evaluated. The results showed that this composite hydrogel not only exhibited excellent pH responsiveness but also demonstrated superior free radical scavenging capability due to the synergistic effects of functional groups such as thiol groups.

参考文献/References:

[1] 孙雨,乐晓霞,路伟,等.功能高分子水凝胶前沿进展与未来趋势[J].前瞻科技,2025,4(1):147-159.
[2] 王少凡,周颖,郝康安,等.具有pH响应性的自愈合蓝光水凝胶[J].化工进展,2023,42(9):4837-4846.DOI:10.16085/j.issn.1000-6613.2022-1944.
[3] 于丁一,李圆圆,王晨钰,等.pH响应性木质素水凝胶的制备及药物控释[J].化工进展,2023,42(6):3138-3146.
[4] ZHANG Yingfeng,WANG Jia.Current status and prospects of gelatin and its derivatives in oncological applications: Review[J].International Journal of Biological Macromolecules,2024,274:133590.DOI:10.1016/j.ijbiomac.2024.133590.
[5] UL-ISLAM M,ALABBOSH K F,MANAN S,et al.Chitosan-based nanostructured biomaterials: Synthesis, properties, and biomedical applications[J].Advanced Industrial and Engineering Polymer Research,2024,7(1):79-99.DOI:10.1016/j.aiepr.2023.07.002.
[6] 姚文杰,辛梅华,李明春,等.pH值响应聚乙二醇化壳聚糖基隐形纳米胶束的制备及其载药性能[J].化工进展,2015,34(1):173-177.
[7] 林意华,李明春,辛梅华,等.O-季铵化-N-(4-十二烷氧基)壳聚糖苯甲醛席夫碱的制备及胶束pH响应性[J].化工进展,2015,34(2):484-489.
[8] SONAWANE S J,KALHAPURE R S,GOVENDER T.Hydrazone linkages in pH responsive drug delivery systems[J].European Journal of Pharmaceutical Sciences,2017,99:45-65.DOI:10.1016/j.ejps.2016.12.011.
[9] FEDERER C,KURPIERS M,BERNKOP-SCHN?RCH A.Thiolated chitosans: A multi-talented class of polymers for various applications[J].Biomacromolecules,2021,22(1):24-56.DOI:10.1021/acs.biomac.0c00663.
[10] KIKANI T,DAVE S,THAKORE S.Functionalization of hyaluronic acid for development of self-healing hydrogels for biomedical applications: A review[J].International Journal of Biological Macromolecules,2023,242:124950.DOI:10.1016/j.ijbiomac.2023.124950.
[11] SHEN K H,CHIU T H,TENG K C,et al.Fabrication of triple-crosslinked gelatin/alginate hydrogels for controlled release applications[J].International Journal of Biological Macromolecules,2023,250:126133.DOI:10.1016/j.ijbiomac.2023.126133.
[12] KISS T,AMBRUS R,ABDELGHAFOUR M M,et al.Preparation and detailed characterization of the thiomer chitosan-cysteine as a suitable mucoadhesive excipient for nasal powders[J].International Journal of Pharmaceutics,2022,626:122188.DOI:10.1016/j.ijpharm.2022.122188.
[13] HOZUMI T,KAGEYAMA T,OHTA S,et al.Injectable hydrogel with slow degradability composed of gelatin and hyaluronic acid cross-linked by schiff’s base formation[J].Biomacromolecules,2018,19(2):288-297.DOI:10.1021/acs.biomac.7b01133.
[14] BAO Zhishu,YU Ailin,SHI Hui,et al.Glycol chitosan/oxidized hyaluronic acid hydrogel film for topical ocular delivery of dexamethasone and levofloxacin[J].International Journal of Biological Macromolecules,2021,167:659-666.DOI:10.1016/j.ijbiomac.2020.11.214.
[15] PENG Yonggang,YU Ying,SU Zhongwen,et al.Novel ionic chitosan derivatives based on pyridinium sulfonate: Synthesis, characterization, and studies on antimicrobial, antioxidant, and biocompatibility properties[J].International Journal of Biological Macromolecules,2025,285:138638.DOI:10.1016/j.ijbiomac.2024.138638.
[16] ZHONG Yujing,YU Ying,PENG Yonggang,et al.Construction of Schiff base dynamically cross-linked pH-responsive chitosan-based hydrogels and investigation of their targeted oral delivery performance for vitamin B12[J].International Journal of Biological Macromolecules,2025,316:144478.DOI:10.1016/j.ijbiomac.2025.144478.
[17] MARTORANA A,LENZUNI M,CONTARDI M,et al.Schiff base-based hydrogel embedded with in situ generated silver nanoparticles capped by a hyaluronic acid-diethylenetriamine derivative for wound healing application[J].ACS Applied Materials & Interfaces,2024,16(16):20186-20201.DOI:10.1021/acsami.4c00657.
[18] YANG Yongyan,MA Ying,WANG Haodong,et al.Chitosan-based hydrogel dressings with antibacterial and antioxidant for wound healing[J].International Journal of Biological Macromolecules,2024,280:135939.DOI:10.1016/j.ijbiomac.2024.135939.

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备注/Memo

备注/Memo:
收稿日期: 2025-10-09
通信作者: 辛梅华(1962-),女,教授,博士,博士生导师,主要从事应用化学的研究。E-mail:mhxin@hqu.edu.cn。
基金项目: 福建省科技计划项目(2019H6014, 2020Y4012)https://hdxb.hqu.edu.cn/
更新日期/Last Update: 2025-11-20