[1]陈明霞,邢元军,李和阳,等.产灵菌红素沙雷氏菌R18的鉴定及基因组特性[J].华侨大学学报(自然科学版),2024,45(5):626-635.[doi:10.11830/ISSN.1000-5013.202311032]
　CHEN Mingxia,XING Yuanjun,LI Heyang,et al.Identification and Genomic Characterization of Prodigiosin-Producing Serratia sp. R18[J].Journal of Huaqiao University(Natural Science),2024,45(5):626-635.[doi:10.11830/ISSN.1000-5013.202311032]
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产灵菌红素沙雷氏菌R18的鉴定及基因组特性()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第5期
页码:
626-635
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Identification and Genomic Characterization of Prodigiosin-Producing Serratia sp. R18
文章编号:
1000-5013(2024)05-0626-10
作者:
陈明霞1;  邢元军1;  李和阳2; 3;  施俊豪1
1. 华侨大学 化工学院, 福建 厦门 361021;2. 自然资源部 第三海洋研究所, 福建 厦门 361005;3. 第三海洋研究所 福建省海洋生态保护与修复重点实验室, 福建 厦门 361005
Author(s):
CHEN Mingxia1;  XING Yuanjun1;  LI Heyang2; 3;  SHI Junhao1
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China; 3. Fujian Provincial Key Laboratory of Marine Ecological Conservation and Restoration, Third Institute of Oceanography, Xiamen 361005, China
关键词:
粘质沙雷氏菌;  灵菌红素;  菌种鉴定;  基因组特性
Keywords:
Serratia marcescens;  prodigiosin;  bacterial identification;  genomic characterization
分类号:
Q939.1
DOI:
10.11830/ISSN.1000-5013.202311032
文献标志码:
A
摘要:
采用形态学、生理生化和分子生物学方法对菌株R18进行菌种鉴定。通过基因组测序和生物信息学比较,进一步分析其分类地位和生物学特性,并在基因组水平上探讨该菌株红色素的合成基因簇和代谢路径。结果表明:菌株R18最佳生长条件为温度30~37 ℃,pH值6~8,NaCl质量浓度0~10 g·L-1;菌株R18与粘质沙雷氏菌模式菌株ATCC 13880的16S rDNA相似度为99.86%,基因组平均核苷酸均一性和数字DNA-DNA杂交值分别为98.73%,89.5%,均高于物种界定阈值,属于同一个物种;菌株R18灵菌红素合成基因簇全长35 021 bp,包含29个基因,其中,4个核心合成基因、10个补充的合成基因、1个调控基因、1个转运基因及13个其他基因,具有完整的灵菌红素合成代谢途径。
Abstract:
Strain R18 was identified using morphological, physiological, biochemical and molecular biological methods. Its taxonomic status and biological properties were further analyzed by comparing genome sequencing and bioinformatics, and the synthetic gene cluster and metabolic pathway of the red pigment in this strain were explored at the genomic level. The results showed that the optimal growth conditions for strain R18 were temperature of 30-37 ℃, pH value of 6-8, and the mass concentration of NaCl of 0-10 g·L-1. Strain R18 shared a 16S rDNA similarity of 99.86% to Serratia marcescens type strain ATCC 13880, the genome average nucleotide identity value and the digital DNA-DNA hybridization value were 98.73% and 89.5%, respectively, both of which were higher than the species identification threshold and belong to the same specie. Strain R18 prodigiosin synthetic gene cluster had a total length of 35 021 bp and contained 29 genes including 4 core syn-thetic genes, 10 complementary synthetic genes, 1 regulatory gene, 1 transporter gene and 13 other genes, it had a complete prodigiosin synthesis metabolic pathway.

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备注/Memo

备注/Memo:
收稿日期: 2023-11-29
通信作者: 李和阳(1978-),男,副研究员,博士,主要从事海洋微生物生态的研究。E-mail:heyang_li@tio.org.cn。
基金项目: 福建省海洋经济发展专项资金资助项目(FJHJF-L-2022-11); 华侨大学2023年实验教学与管理改革课题(SY2023J05); 福建省海洋生态保护与修复重点实验室开放基金资助项目(EPR2020002)https://hdxb.hqu.edu.cn/
更新日期/Last Update: 2024-09-20