[1]金春英,崔亮,陈勇男,等.沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用[J].华侨大学学报(自然科学版),2024,45(5):618-625.[doi:10.11830/ISSN.1000-5013.202311010]
　JIN Chunying,CUI Liang,CHEN Yongnan,et al.Regulatory Role of Arsenic Regulatory Protein(ArsR)in Rhodopseudomonas palustris CGA009[J].Journal of Huaqiao University(Natural Science),2024,45(5):618-625.[doi:10.11830/ISSN.1000-5013.202311010]
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沼泽红假单胞菌CGA009砷调控蛋白(ArsR)的调控作用()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第5期
页码:
618-625
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Regulatory Role of Arsenic Regulatory Protein(ArsR)in Rhodopseudomonas palustris CGA009
文章编号:
1000-5013(2024)05-0618-08
作者:
金春英1;  崔亮1;  陈勇男2;  杨素萍1;  赵春贵1
1. 华侨大学 化工学院, 福建 厦门 361021;2. 北京农学院 国际学院, 北京 102206
Author(s):
JIN Chunying1;  CUI Liang1;  CHEN Yongnan2; YANG Suping1;  ZHAO Chungui1
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. International College, Beijing University of Agricultural, Beijing 102206, China
关键词:
沼泽红假单胞菌;  砷;  砷调控蛋白;  砷生物传感
Keywords:
Rhodopseudomonas palustris;  arsenic;  arsenic regulatory protein;  arsenic biosensing
分类号:
Q811.4
DOI:
10.11830/ISSN.1000-5013.202311010
文献标志码:
A
摘要:
采用改良的凝胶电泳迁移滞缓(EMSA)法和砷生物传感法研究沼泽红假单胞菌CGA009菌株6个高度异质性ArsR相关蛋白的调控作用。研究结果表明:6个ArsR相关蛋白都具有As(Ⅲ)依赖的调控活性,改良的 EMSA图谱可用于系统评价ArsR的功能; 6个相关蛋白对异种(E. coli pR733 or Stapylococcus aureus pⅠ258)的砷抗性操纵子的调控具有不同的选择性,氨基酸序列同源性(甚至低于30%同源性)的ArsR具有砷依赖的调控作用。
Abstract:
Using a modified gel electrophoretic mobility shift assay(EMSA)method and Arsenic biosensing method, the regulation of six highly heterogeneous ArsR-associated proteins from Rhodopseudomonas palustris CGA009 bacterial strain is investigated. The reseach results show that six ArsR-associated proteins exhibited As(Ⅲ)-dependent regulatory activity. The modified EMSA mappings can systematically evaluate the functions of ArsR. Six ArsR-associated proteins have different selectivity in regulating the arsenic resistance operon of different species(E. coli pR733 or Staphylococcus aureus pⅠ258). ArsRs with amino acid sequence homology(even with below 30% homology)have arsenic-dependent regulation.

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备注/Memo

备注/Memo:
收稿日期: 2023-11-01
通信作者: 赵春贵(1970-),男,教授,博士,主要从事资源微生物的挖掘与利用的研究。E-mail:chungui@hqu.edu.cn。
基金项目: 国家海洋公益性行业科研专项(201505026); 福建省自然科学基金资助项目(2018J01049)
更新日期/Last Update: 2024-09-20