[1]林若欣,赵晓岚,赵春贵,等.多拷贝pucBA基因在沼泽红假单胞菌光合生长中的作用[J].华侨大学学报(自然科学版),2024,45(5):610-617.[doi:10.11830/ISSN.1000-5013.202407015]
 LIN Ruoxin,ZHAO Xiaolan,ZHAO Chungui,et al.Role of Multiple Copy pucBA Genes in Phototrophic Growth of Rhodopseudomonas palustris[J].Journal of Huaqiao University(Natural Science),2024,45(5):610-617.[doi:10.11830/ISSN.1000-5013.202407015]
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多拷贝pucBA基因在沼泽红假单胞菌光合生长中的作用()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第45卷
期数:
2024年第5期
页码:
610-617
栏目:
出版日期:
2024-09-20

文章信息/Info

Title:
Role of Multiple Copy pucBA Genes in Phototrophic Growth of Rhodopseudomonas palustris
文章编号:
1000-5013(2024)05-0610-08
作者:
林若欣 赵晓岚 赵春贵 杨素萍
华侨大学 化工学院, 福建 厦门 361021
Author(s):
LIN Ruoxin ZHAO Xiaolan ZHAO Chungui YANG Suping
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
紫细菌 沼泽红假单胞菌 外周捕光复合体(LH2) 基因敲除
Keywords:
purple bacteria Rhodopseudononas palustris peripheral light-harvesting complex(LH2) gene knockout
分类号:
Q78
DOI:
10.11830/ISSN.1000-5013.202407015
文献标志码:
A
摘要:
选择沼泽红假单胞菌CGA009菌株,采用基因敲除方法,依次对菌株中的5对pucBA基因进行敲除,以探求不同pucBA基因对菌株光合生长、光谱表型和光合色素质量浓度的影响。结果表明:不同pucBA基因的表达量与菌株光合生长速率和光合色素合成量呈正相关关系;高光下pucBAb和pucBAa基因对菌株生长发挥主要作用,低光下pucBAb,pucBAd和pucBAa基因对菌株生长起主要作用;敲除pucBAd基因可导致pucBAb基因高效表达,敲除pucBAc基因可导致LH1-RC合成量提高,表明某个pucBA基因的敲除对其他光合基因表达具有补偿作用。
Abstract:
Selecting Rhodopseudomonas palustris CGA009 strain, gene knockout method was used to sequentially knock out five pairs of pucBA genes in the strain in order to explore the effects of different pucBA genes on photosynthetic growth, spectral phenotype and photosynthetic pigment mass concentration of the strain. The results showed that the expression levels of different pucBA genes were positively correlated with the photosynthetic growth rate and photosynthetic pigment synthesis of the strain. The pucBAb and pucBAa genes played a major role in strain growth under high light conditions, while the pucBAb, pucBAd and pucBAa genes played a major role in strain growth under low light conditions. Knocking out the pucBAd gene could lead to efficient expression of pucBAb gene, while knocking out the pucBAc gene could increase LH1-RC synthesis, indicating that the knockout of a certain pucBA gene had a compensatory effect on the expression of other photosynthetic genes.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2024-07-15
通信作者: 杨素萍(1966-),女,教授,博士,主要从事资源微生物的挖掘与利用的研究。E-mail:yangsuping@hqu.edu.cn。
基金项目: 国家自然科学基金资助项目(31070054, 30970068); 国家海洋公益性行业科研专项(201505026)https://hdxb.hqu.edu.cn/
更新日期/Last Update: 2024-09-20