[1]丁飞艳,郑茗冉,吴振,等.柏苓颗粒的降尿酸效果及其初步作用机制[J].华侨大学学报(自然科学版),2023,44(5):615-621.[doi:10.11830/ISSN.1000-5013.202301006]
　DING Feiyan,ZHENG Mingran,WU Zhen,et al.Effect of Bailing Granules on Reducing Uric Acid and Its Preliminary Action Mechanism[J].Journal of Huaqiao University(Natural Science),2023,44(5):615-621.[doi:10.11830/ISSN.1000-5013.202301006]
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柏苓颗粒的降尿酸效果及其初步作用机制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第44卷
期数:
2023年第5期
页码:
615-621
栏目:
出版日期:
2023-09-20

文章信息/Info

Title:
Effect of Bailing Granules on Reducing Uric Acid and Its Preliminary Action Mechanism
文章编号:
1000-5013(2023)05-0615-07
作者:
丁飞艳1;  郑茗冉1;  吴振2;  王立强1;  侯志勇1; 3
1. 华侨大学 生物医学学院, 福建 泉州 362021;2. 厦门大学 药学院, 福建 厦门 361102;3.中国人民解放军联勤保障部队第962医院, 黑龙江 哈尔滨 150086
Author(s):
DING Feiyan1;  ZHENG Mingran1;  WU Zhen2; WANG Liqiang1;  HOU Zhiyong1; 3
1. School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China; 2. School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China; 3. The 962nd Hospital of the Joint Service Support Force of the Chinese People’s Liberation Army,
关键词:
柏苓颗粒;  高尿酸血症;  黄嘌呤氧化酶;  有机阴离子转运体1
Keywords:
Bailing granules;  hyperuricemia;  xanthine oxidase;  organic anion transporter 1
分类号:
R971.1
DOI:
10.11830/ISSN.1000-5013.202301006
文献标志码:
A
摘要:
将42只雄性大鼠随机分为空白组、模型组、阳性对照组(非布司他组)、柏苓汤剂组及柏苓颗粒高、中、低剂量组,使用氧嗪酸钾灌胃,建立高尿酸血症(HUA)大鼠模型,连续给药14 d,每天1次.采用酶联免疫吸附法测定血清与肝脏黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)活性,血清中尿酸(SUA)、肌酐(Cr)、尿素氮(BUN)水平及肝脏次黄嘌呤鸟嘌呤磷酸核糖转移酶(HGPRT)活性.采用蛋白免疫印迹法测定肾脏中有机阴离子转运体1(OAT1)及尿酸阴离子转运体1(URAT1)蛋白的相对表达水平.结果表明:与模型组相比,柏苓颗粒可显著降低SUA水平、XOD和ADA活性,趋势呈剂量依赖性;所有组别大鼠的血清Cr和BUN浓度及肝脏HGPRT活性无统计学意义;柏苓颗粒高剂量组能极显著抑制URAT1蛋白相对表达水平并上调OAT1蛋白的相对表达水平,作用与柏苓汤剂组和非布司他组相当.
Abstract:
42 male rats were randomly divided into blank group, model group, positive control group(febuxostat group), Bailing decoction group and high, medium and low dose groups of Bailing granules.The rat model of hyperuricemia(HUA)was established by intragastric administration of potassium oxonate once a day for 14 days. Activities of xanthine oxidase(XOD)and adenosine deaminase(ADA)in serum and liver, levels of serumuric acid(SUA), creatinine(Cr)and urea nitrogen(BUN)in serum and the activity of hypoxanthine guanine phosphoribosyltransferase(HGPRT)in liver were measured by enzyme linked immunosorbent assay method. The relative protein expression levels of organic anion transporter 1(OAT1)and uric acid anion transporter 1(URAT1)in the kidney were determined by protein immunoblotting method. The results show that, compared with model group, Bailing granules can significantly reduce SUA level, XOD and ADA activities, and the trend is dose-dependent. There are no statistical significance in concentration of Cr and BUN in serum and the activity of HGPRT in liver among all groups of rats. The high-dose group of Bailing granules can significantly inhibit the relative expression level of URAT1 protein and up-regulate the relative expression level of OAT1 protein, which are equivalent to that of Bailing decoction group and febuxostat group.

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备注/Memo

备注/Memo:
收稿日期: 2023-01-06
通信作者: 王立强(1970-),男,教授,博士,主要从事药剂学和创新药物研发的研究.E-mail:wlq1599@163.com.
基金项目: 国家重点研发计划项目(2016YFF0101700); 福建省高校产学合作重大项目(2019Y4007)http://www.hdxb.hqu.edu.cn
更新日期/Last Update: 2023-09-20