[1]黄亦欣,邹景,林金彬,等.ABTS强化HRP/H2O2体系降解吲哚的效能与机制[J].华侨大学学报(自然科学版),2022,43(6):753-762.[doi:10.11830/ISSN.1000-5013.202203052]
　HUANG Yixin,ZOU Jing,LIN Jinbin,et al.Efficiency and Mechanism of ABTS Enhanced HRP/H2O2 System for Indole Degradation[J].Journal of Huaqiao University(Natural Science),2022,43(6):753-762.[doi:10.11830/ISSN.1000-5013.202203052]
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ABTS强化HRP/H2O2体系降解吲哚的效能与机制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第43卷
期数:
2022年第6期
页码:
753-762
栏目:
出版日期:
2022-11-11

文章信息/Info

Title:
Efficiency and Mechanism of ABTS Enhanced HRP/H2O2 System for Indole Degradation
文章编号:
1000-5013(2022)06-0753-10
作者:
黄亦欣;  邹景;  林金彬;  马红芳;  陈秀峰;  杨浩宇
华侨大学 土木工程学院, 福建 厦门 361021
Author(s):
HUANG Yixin;  ZOU Jing;  LIN Jinbin;  MA Hongfang;  CHEN Xiufeng;  YANG Haoyu
College of Civil Engineering, Huaqiao University, Xiamen 361021, China
关键词:
辣根过氧化物酶;  ABTS;  过氧化氢;  ABTS自由基;  吲哚
Keywords:
horseradish peroxidase;  ABTS;  hydrogen peroxide;  ABTS radicals;  indole
分类号:
X703.1
DOI:
10.11830/ISSN.1000-5013.202203052
文献标志码:
A
摘要:
针对辣根过氧化物酶催化过氧化氢(HRP/H2O2)体系存在对污染物降解速度慢的问题,采用2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)作为电子转移体,强化HRP/H2O2体系降解吲哚,对不同pH值、ABTS浓度和常见共存水体成分的吲哚降解效能进行研究.通过高效液相色谱-四级杆-飞行时间质谱仪及发光细菌毒性实验,考察吲哚的降解产物及毒性变化.结果表明:在pH值为5.0~11.0的范围内,ABTS可显著强化HRP/H2O2体系降解吲哚,且强化效能随ABTS浓度的增加而增加;常见共存水体成分对吲哚的降解均无显著影响;检测到5种吲哚降解产物,其生物毒性相较于吲哚有所下降.
Abstract:
Aiming at the problem of slow degradation rate of pollutants in horseradish peroxidase catalyzed hydrogen peroxide(HRP/H2O2)system,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate)(ABTS)was used as electron shuttle to enhance the degradation of indole in HRP/H2O2 system. The degradation efficiency of indole in different pH values, ABTS concentrations and common coexisting water components were investigated. The degradation products and toxicity changes of indole were investigated by high performance liquid chromatography-quadrupole-time of flight mass spectrometry and luminescent bacteria toxicity test. The results showed that ABTS could significantly enhance the degradation of indole in HRP/H2O2 system in the pH range of 5.0-11.0,and the enhancement efficiency increased with the increase of ABTS concentration. The common coexisting water components had no significant effect on the degradation of indole. Five indole degradation products were detected, and their biological toxicity decreased compared with indole.

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备注/Memo

备注/Memo:
收稿日期: 2022-03-22
通信作者: 邹景(1986-),男,副教授,博士,主要从事高级氧化水处理新技术与理论的研究.E-mail:zoujing@hqu.edu.cn.
基金项目: 国家级大学生创新创业训练计划项目(202110385031); 福建省厦门市青年创新基金资助项目(3502Z20 206007); 华侨大学中青年教师科研提升资助计划项目(ZQN-YX506)http://www.hdxb.hqu.edu.cn
更新日期/Last Update: 2022-11-20