[1]邱岳,张丹丹,吴姝羽,等.蝌蚪状类弹性蛋白多肽相变特性分析[J].华侨大学学报(自然科学版),2018,39(3):414-419.[doi:10.11830/ISSN.1000-5013.201712002]
　QIU Yue,ZHANG Dandan,WU Shuyu,et al.Phase Transition Behavior of Tadpole-Like Elastin-Like Polypeptides[J].Journal of Huaqiao University(Natural Science),2018,39(3):414-419.[doi:10.11830/ISSN.1000-5013.201712002]
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蝌蚪状类弹性蛋白多肽相变特性分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第39卷
期数:
2018年第3期
页码:
414-419
栏目:
出版日期:
2018-05-20

文章信息/Info

Title:
Phase Transition Behavior of Tadpole-Like Elastin-Like Polypeptides
文章编号:
1000-5013(2018)03-0414-06
作者:
邱岳;  张丹丹;  吴姝羽;  蔡征文;  林源清;  张光亚
华侨大学 化工学院, 福建 厦门 361021
Author(s):
QIU Yue;  ZHANG Dandan;  WU Shuyu;  CAI Zhengwen; LIN Yuanqing;  ZHANG Guangya
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
蛋白拓扑结构;  蝌蚪状ELPs40;  相变温度;  Hofmeister离子序;  构效关系
Keywords:
protein topology;  tadpole-like ELPs40;  phase transition temperature;  Hofmeister ion sequence;  structure-activity relationship
分类号:
Q35
DOI:
10.11830/ISSN.1000-5013.201712002
文献标志码:
A
摘要:
为研究不同盐类型及浓度对蝌蚪状ELPs40相变特性的影响,探究霍夫曼斯特(Hofmeister)离子序中阴、阳离子对蝌蚪状ELPs40相变温度的影响,通过设计、表达和纯化后,获得由谍标签/捕谍器(SpyTag/SpyCatcher),以及由40个重复的线性类弹性蛋白多肽所形成的2个蝌蚪型结构的ELPs40.结果表明:蝌蚪状ELPs40相变温度比ELPs80相变温度高1.35 ℃;阴离子对蝌蚪状ELPs40相变温度的影响符合Hofmeister离子序现象,但NH4+,K+,Na+等阳离子却发生逆Hofmeister离子序现象;在NaCl,Na2SO4,NaNO3,K2SO4,KCl,KNO3,NH4Cl,NH4NO3,(NH4)2SO4等不同浓度缓冲液中,蝌蚪状ELPs40发生相变所需的温度范围显著高于线性ELPs40,这与其特殊的结构密切相关.
Abstract:
In order to study the effects of different salt types and concentrations on the phase transition characteristics of elastin-like polypeptides 40(ELPs40), the influence of the anions and cations in the Hofmeister on the phase transition temperature of ELPs40 was investigated. After designing, expressing and purifying, the ELPs40 with the SpyTag/SpyCatcher and two 40-repetitive linear elastin-like peptides were obtained. The results show that the phase transition temperature of ELPs40 is higher with 1.35 ℃ than that of the ELPs80. The effect of anions on the phase transition temperature of ELPs40 accords with Hofmeister, but the cations such as NH4+, K+, Na+ have inverse Hofmeister; in the buffers with different concentrations such as NaCl, Na2SO4, NaNO3, K2SO4, KCl, KNO3, NH4Cl, NH4NO3,(NH4)2SO4, etc., temperature range required for phase transition of the tadpole-like ELPs40 is significantly higher than that of the linear ELPs40, which should be closely related to its special structure.

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备注/Memo

备注/Memo:
收稿日期: 2017-12-04
通信作者: 张光亚(1975-),男,教授,博士,主要从事酶与智能多肽的研究.E-mail:zhgyghh@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(21376103); 福建省自然科学基金资助项目(2017J01065)
更新日期/Last Update: 2018-05-20