[1]梅胜,王镇发,罗云,等.阿魏酸酯酶在体外模拟猪胃肠道中的稳定性[J].华侨大学学报(自然科学版),2017,38(5):659-663.[doi:10.11830/ISSN.1000-5013.201608020]
　MEI Sheng,WANG Zhenfa,LUO Yun,et al.Stability of Ferulic Acid Esterase in Simulated Gastrointestinal Tract of Pigs[J].Journal of Huaqiao University(Natural Science),2017,38(5):659-663.[doi:10.11830/ISSN.1000-5013.201608020]
点击复制

阿魏酸酯酶在体外模拟猪胃肠道中的稳定性()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第38卷
期数:
2017年第5期
页码:
659-663
栏目:
出版日期:
2017-09-20

文章信息/Info

Title:
Stability of Ferulic Acid Esterase in Simulated Gastrointestinal Tract of Pigs
文章编号:
1000-5013(2017)05-0659-05
作者:
梅胜1;  王镇发2;  罗云1;  陈培钦1;  李夏兰1
1. 华侨大学 化工学院, 福建 厦门 361021;2. 漳州市龙文区环境保护监测站, 福建 漳州 363005
Author(s):
MEI Sheng1;  WANG Zhenfa2;  LUO Yun1;  CHEN Peiqin1;  LI Xialan1
1. College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2. Longwen District Environmental Protection Monitoring Station of Zhangzhou, Zhangzhou 363005, China
关键词:
阿魏酸酯酶;  饲用酶;  胃肠道;  酶活
Keywords:
ferulic acid esterase;  feed enzyme;  gastrointestinal tract;  enzyme activity
分类号:
Q95.33
DOI:
10.11830/ISSN.1000-5013.201608020
文献标志码:
A
摘要:
模拟了胃(肠)液中pH值、蛋白酶、进食量和微量金属离子等对阿魏酸酯酶(FAE)酶活稳定性的影响.结果表明:模拟胃液环境对阿魏酸酯酶酶活影响显著,模拟肠液环境对阿魏酸酯酶稳定性影响较小;蛋白酶酶活的增加不利于阿魏酸酯酶稳定性,但影响不显著;混合金属离子对阿魏酸酯酶稳定性影响不显著;进食量增加可以保护阿魏酸酯酶通过胃液环境,进入小肠发挥作用.在模拟胃液中,阿魏酸酯酶的酶活迅速降低,1 h时,酶活为25%左右,调整为小肠环境时,对阿魏酸酯酶有瞬时激活作用;5 h时,酶活为30%左右;胃肠道pH值是影响阿魏酸酯酶酶活的主要因素,胃蛋白酶、肠道中的金属离子对阿魏酸酯酶酶活影响较小,进食量对肠道中阿魏酸酯酶有保护作用,模拟肠液对经过模拟胃液处理的阿魏酸酯酶有瞬时激活作用.
Abstract:
In this paper, we simulated the influence of pH, protease, food-intake and trace metal ions in the gastrointestinal fluid on the stability of ferulic acid esterase(FAE)activity. The results showed the simulated gastric fluid environment had significant effects on the activity of FAE, whereas the intestinal juice had subtle effects on the stability of FAE. The increasing activity of protease was unfavorable to the stability of FAE, which was not obvious. Similarly, the effect of mixed metal ions on the stability of FAE was not significant. The increasing food-intake could protect FAE from degradation in the gastric environment and make it work in the small intestine. Furthermore, the activity of FAE in the simulated gastric fluid decreased rapidly, which was about 25% of the initial activity in one hour. As for the intestine environment, it had a transient activation to FAE with 30% of the initial activity in five hours. In summary, the pH of the gastrointestinal tract is the key factor that affects the activity of FAE. Both the protease and the metal ions in intestine have a little influence on the activity of FAE. Besides, the food-intake plays a protective role in FAE. And the simulated intes-tinal juice has instant activation on the FAE treated with the simulated gastric juice.

参考文献/References:

[1] BRAGA C M,DELABONA P S,LIMA D J,et al.Addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis[J].Bioresource Technology,2014,170(5):316-324.
[2] BARTOLOME B,GOMEZ C C,SANCHO A I,et al.Growth and release of hydroxycinnamic acids from Brewer’s spent grain by streptomyces avermitilis CECT 3339[J].Enzyme and Microbial Technology,2007,32(1):140-144.
[3] SELIG M J,KNOSHAUG E P,ADNEY W S,et al.Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities[J].Bioresource Technology, 2008,99(11):4997-5005.
[4] 杨道秀,李夏兰,陈培钦,等.新型复合酶在肉鸡饲料中的应用[J].食品与生物技术学报,2013,32(4):410-416.
[5] MATHEW S,ABRAHAM T E.Studies on the production of feruloyl esterase from cereal brans and sugar cane bagasse by microbial fermentation[J].Enzyme and Microbial Technology,2005,36(4):565-570.
[6] 杨红建,黎大洪,谢春元,等.阿魏酸酯酶处理对草、玉米秸、稻秸及麦秸瘤胃体外发酵特性的影响[J].动物营养学报,2010,22(1):207-211.
[7] 郝志敏.饲用酶制剂的开发应用及存在的问题[J].饲料与畜牧,2010(11):25-27.
[8] 张伟,詹志春.饲用酶制剂研究进展与发展趋势[J].饲料工业,2011(增刊1):11-19.
[9] YUE Q,YANG H J,LI D H,et al.A comparison of HPLC and spectrophotometrical methods to determine the activity of ferulic acid esterase in commercial enzyme products and rumen contents of steers[J].Anim Feed Sci Tech,2009,153(3/4):169-177.
[10] MATHEW S,ABRAHAM T E.Studies on the production of feruloyl esterase from cereal brans and sugar cane bagasse by microbial fermentation[J].Enzyme Microb Tech,2005,36(4):565-570.
[11] MILLER L C.The United States pharmacopeia[M].Rockville: United States Pharmacopeial Convention, 1983:510-530.
[12] STRUBE M L,MEYER A S,BOYE M.Minireview: Basic physiology and factors influencing exogenous enzymes activity in the porcine gastrointestinal tract[J].Animal Nutrition and Feed Technology,2013(13):441-459.
[13] 孙建义,李卫芬,顾赛红.体外模拟动物肠胃条件下β-葡聚糖酶稳定性的研究[J].中国畜牧志,2002,38(1):18-19.
[14] 杨道秀.阿魏酸酯酶在麦糟酶化饲料中的应用[D].厦门:华侨大学,2013:20-23.
[15] MORGAVI D P,BEAUCHEMIN K A,NSEREKO V L,et al.Resistance of feed enzymes to proteolytic inactivation by rumen microorganisms and gastrointestinal proteases[J].Journal of Animal Science,2001,79(6):1621-1630.

相似文献/References:

[1]范韵敏,李夏兰,方柏山.阿魏酸酯酶产生菌的培养条件优化[J].华侨大学学报(自然科学版),2010,31(4):426.[doi:10.11830/ISSN.1000-5013.2010.04.0426]
　FAN Yun-min,LI Xia-lan,FANG Bai-shan.Optimization of Fermentation Conditions of Ferulic Acid Esterase Producers[J].Journal of Huaqiao University(Natural Science),2010,31(5):426.[doi:10.11830/ISSN.1000-5013.2010.04.0426]
[2]王镇发,陈培钦,邓秩韬,等.反胶束萃取阿魏酸酯酶[J].华侨大学学报(自然科学版),2013,34(2):182.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
　WANG Zhen-fa,CHEN Pei-qin,DENG Yi-tao,et al.Purification of Feruloyl Esterase Using Reverse Micelles Extraction[J].Journal of Huaqiao University(Natural Science),2013,34(5):182.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
[3]王林林,陈云华,陈培钦,等.阿魏酸酯酶酶化发酵饲料制备及其对肉鸡养分利用率的影响[J].华侨大学学报(自然科学版),2014,35(6):689.[doi:10.11830/ISSN.1000-5013.2014.06.0689]
　WANG Lin-lin,CHEN Yun-hua,CHEN Pei-qin,et al.Preparation of Ferulic Acid Esterase Enzymatic Fermented Feed andIts Effect on the Nutrient Utilization of Broilers[J].Journal of Huaqiao University(Natural Science),2014,35(5):689.[doi:10.11830/ISSN.1000-5013.2014.06.0689]
[4]陈云华,李慧,张光亚,等.阿魏酸酯酶O42807在毕赤酵母GS115中的表达[J].华侨大学学报(自然科学版),2016,37(2):224.[doi:10.11830/ISSN.1000-5013.2016.02.0224]
　CHEN Yunhua,LI Hui,ZHANG Guangya,et al.Expression of Feruloyl Esterase O42807 in Pichia pastoris GS115[J].Journal of Huaqiao University(Natural Science),2016,37(5):224.[doi:10.11830/ISSN.1000-5013.2016.02.0224]

备注/Memo

备注/Memo:
收稿日期: 2016-08-19
通信作者: 李夏兰(1965-),女,教授,博士,主要从事生物化工的研究.E-mail:xialan@hqu.edu.cn.
基金项目: 福建省发改委投资项目(2013-886)http://www.hdxb.hqu.edu.cn
更新日期/Last Update: 2017-09-20