[1]周耕民,刁勇,李三暑.核酶的发现及其在基因治疗中的应用[J].华侨大学学报(自然科学版),2017,38(4):509-514.[doi:10.11830/ISSN.1000-5013.201704012]
 ZHOU Gengmin,DIAO Yong,LI Sanshu.Discovery of Ribozymes and Their Application in Gene Therapy[J].Journal of Huaqiao University(Natural Science),2017,38(4):509-514.[doi:10.11830/ISSN.1000-5013.201704012]
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核酶的发现及其在基因治疗中的应用()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第38卷
期数:
2017年第4期
页码:
509-514
栏目:
出版日期:
2017-07-10

文章信息/Info

Title:
Discovery of Ribozymes and Their Application in Gene Therapy
文章编号:
1000-5013(2017)04-0509-06
作者:
周耕民 刁勇 李三暑
华侨大学 生物医学学院, 福建 泉州 362021
Author(s):
ZHOU Gengmin DIAO Yong LI Sanshu
School of Biomedical Sciences, Huaqiao University, Quanzhou 362021, China
关键词:
核酶 非编码RNA 基因治疗 基因表达 剪切RNA
Keywords:
ribozyme non-coding RNA gene therapy gene expression cut RNA
分类号:
Q7
DOI:
10.11830/ISSN.1000-5013.201704012
文献标志码:
A
摘要:
核酶是具有催化功能的结构性RNA分子,且大多数核酶具有剪切RNAs的功能,可以利用它们剪切信使RNA(mRNAs)调节基因表达,从而作为基因治疗的新手段.阐述核酶的发现历程,以及其在艾滋病、肝炎、肿瘤和生殖道系统感染等基因治疗的研究进展.最后,分析核酶在基因治疗中的技术优势及存在问题,并对核酶领域包括更多核酶晶体结构的确立、酶切机理的理解、核酶新的生物学功能的发现等研究进行展望.
Abstract:
Ribozymes are structured RNA molecules with catalytic activities. Since most ribozymes have the ability to cleave RNAs, they could be used to cleave mRNAs and regulate gene expression, and therefore becoming new tools for gene therapy. This paper describes the discovery history of ribozymes and their applications in the therapy researches of acquired immune deficiency syndrome, hepatitis, tumor, reproductive tract infection and other diseases. Lastly, the paper analyzes the technological advantages and the existing problems of the ribozyme’s application in gene therapy, and looks into the future of researches on the establishment of more crystal structures of new ribozymes, understanding of ribozyme cleavage mechanisms, and the discovery of new biological functions of ribozymes.

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备注/Memo

备注/Memo:
收稿日期: 2017-03-30
通信作者: 李三暑(1972-),男,教授,博士,主要从事核酶和核糖开关的研究.E-mail:sanshuli@126.com.
基金项目: 国家自然科学基金资助项目(81271691, 81371669); 华侨大学高层次人才科研启动项目(Z16Y0015); 华侨大学研究生科研创新能力培育计划资助项目(1511316013)
更新日期/Last Update: 2017-07-20