[1]陈丹,连惠婷,孙向英,等.酪氨酸分子印迹电化学传感器的制备及性能[J].华侨大学学报(自然科学版),2015,36(预先出版):0.
　CHEN Dan,LIAN Hui-ting,SUN Xiang-ying,et al.Preparation of Tyrosine Molecularly Imprinted Electrochemical Sensor and the Investigation of Its Characters[J].Journal of Huaqiao University(Natural Science),2015,36(预先出版):0.
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酪氨酸分子印迹电化学传感器的制备及性能
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第36卷
期数:
2015年预先出版
页码:
0
栏目:
出版日期:
2027-07-30

文章信息/Info

Title:
Preparation of Tyrosine Molecularly Imprinted Electrochemical Sensor and the Investigation of Its Characters
作者:
陈丹;  连惠婷;  孙向英;  刘斌
华侨大学 材料科学与工程学院, 福建 厦门 361021
Author(s):
CHEN Dan;  LIAN Hui-ting;  SUN Xiang-ying;  LIU Bin
College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China
关键词:
L-酪氨酸;  分子印迹;  电化学传感器;  壳聚糖
Keywords:
tyrosine;  molecular imprinted polymer;  electrochemical sensor;  chitosan
分类号:
O657.1;TP212.2
文献标志码:
A
摘要:
采用恒电位沉积法,制备以L-酪氨酸为模板分子,壳聚糖为功能基体的分子印迹电化学传感器.实验结果表明:在优化的制备和测试条件下,所制得的印迹传感器对L-酪氨酸具有良好的特异识别性能,印迹因子达3.34;在pH值为6.0,0.1 mol·L-1的磷酸盐缓冲溶液中,L-酪氨酸的微分脉冲伏安氧化峰峰电流与浓度在4.0×10-7~1.0×10-4 mol·L-1范围内呈良好的线性关系,检出限为2.0×10-7 mol·L-1.将该传感器用于人血清中酪氨酸含量的测定,平均回收率为89.50%~99.67%.
Abstract:
A novel molecularly imprinted electrochemical sensor was prepared with chitosan as functional matrix and L-tyrosine(L-Tyr)as template molecule by potentiostatic electrodeposition. According to our expectation, the MIP sensor with an imprinted factor of 3.34 showed well sensitivity and good selectivity to L-Tyr among the structural similarities and co-existences. The oxidation peak currents of differential pulse voltammetry were linear to the concentration of L-Tyr in the range from 4.0×10-7 to 1.0×10-4 mol·L-1 with a detection limit of 2.0×10-7 mol·L-1. Furthermore, the recoveries ranged from 89.50% to 99.67% were obtained when the sensor was applied to real samples analysis.

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备注/Memo

备注/Memo:
收稿日期: 2013-12-20
通信作者: 刘斌(1963-),男,教授,主要从事电化学传感器的研究.E-mail:bliu@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(20955001, 21175049, 21275059); 福建省自然科学基金计划资助项目(2011J01049, 2012J01044, 2013J01047); 中央高校基本科研业务费专项资金资助项目(JB-ZR1215)
更新日期/Last Update: 1900-01-01