[1]陈丹,连惠婷,孙向英,等.酪氨酸分子印迹电化学传感器的制备及性能[J].华侨大学学报(自然科学版),2014,35(4):403-408.[doi:10.11830/ISSN.1000-5013.2014.04.0403]
　CHEN Dan,LIAN Hui-ting,SUN Xiang-ying,et al.Preparation and Properties of Tyrosine Molecularly Imprinted Electrochemical Sensor[J].Journal of Huaqiao University(Natural Science),2014,35(4):403-408.[doi:10.11830/ISSN.1000-5013.2014.04.0403]
点击复制

酪氨酸分子印迹电化学传感器的制备及性能()
分享到：

《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第35卷
期数:
2014年第4期
页码:
403-408
栏目:
出版日期:
2014-07-10

文章信息/Info

Title:
Preparation and Properties of Tyrosine Molecularly Imprinted Electrochemical Sensor
文章编号:
1000-5013(2014)04-0403-06
作者:
陈丹;  连惠婷;  孙向英;  刘斌
华侨大学 材料科学与工程学院, 福建 厦门 361021
Author(s):
CHEN Dan;  LIAN Hui-ting;  SUN Xiang-ying;  LIU Bin
College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China
关键词:
L-酪氨酸;  分子印迹;  电化学传感器;  壳聚糖
Keywords:
L-tyrosine;  molecular imprinting;  electrochemical sensor;  chitosan
分类号:
O657.1;TP212.2
DOI:
10.11830/ISSN.1000-5013.2014.04.0403
文献标志码:
A
摘要:
采用恒电位沉积法,制备以L-酪氨酸为模板分子,壳聚糖为功能基体的分子印迹电化学传感器.实验结果表明:在优化的制备和测试条件下,所制得的印迹传感器对L-酪氨酸具有良好的特异识别性能,印迹因子达3.34;在pH值为6.0,0.1 mol·L-1的磷酸盐缓冲溶液中,L-酪氨酸的微分脉冲伏安氧化峰峰电流与浓度在4.0×10-7~1.0×10-4 mol·L-1范围内呈良好的线性关系,检出限为2.0×10-7 mol·L-1.将该传感器用于人血清中酪氨酸含量的测定,平均回收率为89.50%~99.67%.
Abstract:
A novel molecularly imprinted electrochemical sensor was prepared with chitosan as functional matrix and L-tyrosine(L-Tyr)as template molecule by potentiostatic electrodeposition. According to our experimental results, the molecular imprinted polymer(MIP)sensor with an imprinted factor of 3.34 showed well sensitivity and good selectivity to L-Tyr among the structural similarities and co-existences. The oxidation peak currents of differential pulse voltammetry were linear to the concentration of L-Tyr in the range from 4.0×10-7 to 1.0×10-4 mol·L-1 with a detection limit of 2.0×10-7 mol·L-1. Furthermore, the recoveries ranged from 89.50% to 99.67% were obtained when the sensor was applied to real samples analysis.

参考文献/References:

[1] CARLSSON A,LINDQVIST M,ARCH N S.Dependence of 5-HT and catecholamine synthesis on concentrations of precursor amino-acids in rat brain[J].Pharmacology,1978,303(2):157-164.
[2] BOOMSMA F,MEIRACKER A,SCHALEKAMP M.Contrasting effects of peripheral decarboxylase inhibitors on plasma activity of aromatic-L-amino acid decarboxylase and semicarbazide-sensitive amine oxidase in Parkinson’s disease[J].Life Sci,1995,57(19):1753-1759.
[3] 邹忠梅,喻长远.人体生命之源(氨基酸)[M].北京:中国医药科技出版社,2000:76-77.
[4] GÖTZE L,HEGELE A,METZELDER S K,et al.Development and clinical application of a LC-MS/MS method for simultaneous determination of various tyrosine kinase inhibitors in human plasma[J].Clin Chim Acta,2012,413(1/2):143-149.
[5] CHRISTOPHER D A,NICOLAS G,STANISLAV R S.Automated method for analysis of tryptophan and tyrosine metabolites using capillary electrophoresis with native fluorescence detection[J].Anal Bioanal Chem.2013,405(8):2451-2459.
[6] DENG Chun-hui,DENG Yong-hui,WANG Bin,et al.Gas chromatography-mass spectrometry method for determination of phenylalanine and tyrosine in neonatal blood spots[J].J Chromatogr B,2002,780(2):407-413.
[7] CHRASTIL J.Spectrophotometric determination of tryptophan and tyrosine in peptides and proteins based on new color reactions[J].Anal Biochem,1986,158(2):443-446.
[8] 魏薇,王洪鉴,江崇球,等.新型荧光试剂1,5-二(4,6-二氯三嗪)-氨基萘与酪氨酸相互作用[J].分析化学,2007,35(12):1772-1775.
[9] ZHU Xia-shi,XU Su-qin.Determination of L-tyrosine by β-cyclodextrin sensitized fluorescence quenching method[J].Spectrochim Acta Part A,2010,77(3):566-571.
[10] SAUMYA V,PRATHISH K P,RAO T P.In situ copper oxide modified molecularly imprinted polypyrrole film based voltammetric sensor for selective recognition of tyrosine[J].Talanta,2011,85(2):1056-1062.
[11] TANG Xiao-feng,LIU Yang,HOU Hao-qing,et al.Electrochemical determination of L-tryptophan, L-tyrosine and L-cysteine using electrospun carbon nanofibers modified electrode[J].Talanta,2010,80(5):2182-2186.
[12] JIANG Lin,GU Su-qing,DING Ya-ping,et al.Amperometric sensor based on tricobalt tetroxide nanoparticles-graphene nanocomposite film modified glassy carbon electrode for determination of tyrosine[J].Colloids and Surfaces B: Biointerfaces,2013,107(1):146-151.
[13] SRIVASTAVA,ASHWINI K,GAICHORE R R.Simultaneous determination of L-tyrosine and caffeine based on their electrocatalytic oxidation at a 4-tert-butylcalix[6] arene-modified carbon paste electrode[J].Journal of AOAC International,2013,96(1):133-141.
[14] GHOREISHI S M,BEHPOUR M,JAFARI N,et al.Electrochemical determination of tyrosine in the presence of dopamine and uric acid at the surface of gold nanoparticles modified carbon paste electrode[J].J Chin Chem Soc,2012,59(8):1015-1020.
[15] LIU Xiao,LUO Li-qiang,DING Ya-ping,et al.Simultaneous determination of L-cysteine and L-tyrosine using Au-nanoparticles/poly-eriochrome black T film modified glassy carbon electrode[J].Bioelectrochemistry,2012,86(2012):38-45.
[16] 周丰,连惠婷,曹学功,等.掺杂石墨烯分子印迹传感器对色氨酸的手性识别[J].华侨大学学报:自然科学版,2012,33(5):528-534.
[17] 孙兆辉,连惠婷,孙向英,等.石墨烯掺杂对分子印迹电化学传感器的增敏作用[J].华侨大学学报:自然科学版,2012,33(4):401-406.
[18] LIU Bin,WANG Xiao-li,LIAN Hui-ting,et al.Xanthine microsensor based on polypyrrole molecularly imprinted film modified carbon fiber microelectrodes[J].Anal Biochem,2013,440(2):220-226.
[19] 姜忠义,吴洪.分子印迹技术[M].北京:化学工业出版社,2003:1-3.
[20] PILLETSKY S A,TURNER A P F.Electrochemical sensors based on molecularly imprinted polymers[J].Electroanalysis,2002,14(5):317-323.
[21] BKABCI-LOPEZ M C,LOBO-CASTANON M J,MIRANDA-ORDIERES A J,et al.Electrochemical sensors based on molecularly imprinted polymers[J].TrAC Trends Anal Chem,2004,23(1):36-48.
[22] 农兰平,黄敏,庄玉萍.L-色氨酸分子印迹壳聚糖膜的制备及透过选择性[J].化学研究,2009,20(3):15-18.
[23] 吴洪,赵艳艳,喻应霞,等.分子印迹壳聚糖膜分离手性苯丙氨酸[J].功能高分子学报,2007,19/20(2):262-266.

相似文献/References:

[1]杨芸,连惠婷,刘斌,等.敌百虫分子印迹聚合物的合成及其性能[J].华侨大学学报(自然科学版),2009,30(3):284.[doi:10.11830/ISSN.1000-5013.2009.03.0284]
　YANG Yun,LIAN Hui-ting,LIU Bin,et al.Preparation and Properties of Trichlorfon Molecularly Imprinted Polymers[J].Journal of Huaqiao University(Natural Science),2009,30(4):284.[doi:10.11830/ISSN.1000-5013.2009.03.0284]
[2]殷静芬,连惠婷,孙向英,等.尿酸分子印迹电化学传感器的研制及其应用[J].华侨大学学报(自然科学版),2012,33(1):33.[doi:10.11830/ISSN.1000-5013.2012.01.0033]
　YIN Jing-fen,LIAN Hui-ting,SUN Xiang-ying,et al.Highly Selective Determination of Uric Acid in Human Serum by Molecularly Imprinted Electrochemical Sensor[J].Journal of Huaqiao University(Natural Science),2012,33(4):33.[doi:10.11830/ISSN.1000-5013.2012.01.0033]
[3]孙兆辉,连惠婷,孙向英,等.石墨烯掺杂对分子印迹电化学传感器的增敏作用[J].华侨大学学报(自然科学版),2012,33(4):401.[doi:10.11830/ISSN.1000-5013.2012.04.0401]
　SUN Zhao-hui,LIAN Hui-ting,SUN Xiang-ying,et al.Sensitivity Enhancement of Graphene Doped Molecular Imprinting Electrochemical Sensors[J].Journal of Huaqiao University(Natural Science),2012,33(4):401.[doi:10.11830/ISSN.1000-5013.2012.04.0401]
[4]陈丹,连惠婷,孙向英,等.酪氨酸分子印迹电化学传感器的制备及性能[J].华侨大学学报(自然科学版),2015,36(预先出版):0.
　CHEN Dan,LIAN Hui-ting,SUN Xiang-ying,et al.Preparation of Tyrosine Molecularly Imprinted Electrochemical Sensor and the Investigation of Its Characters[J].Journal of Huaqiao University(Natural Science),2015,36(4):0.

备注/Memo

备注/Memo:
收稿日期: 2013-12-20
通信作者: 刘斌(1963-),男,教授,主要从事电化学传感器的研究.E-mail:bliu@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(20955001, 21175049, 21275059); 福建省自然科学基金计划资助项目(2011J01049, 2012J01044, 2013J01047); 中央高校基本科研业务费专项资金资助项目(JB-ZR1215)
更新日期/Last Update: 2014-07-20