[1]黄雅燕,王文平,肖美添.碱性蛋白酶水解豆粕制备大豆多肽[J].华侨大学学报(自然科学版),2013,34(6):674-677.[doi:10.11830/ISSN.1000-5013.2013.06.0674]
　HUANG Ya-yan,WANG Wen-ping,XIAO Mei-tian.Production of Soybean Peptide by Alkaline Protease Hydrolysis of Soybean Meal[J].Journal of Huaqiao University(Natural Science),2013,34(6):674-677.[doi:10.11830/ISSN.1000-5013.2013.06.0674]
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碱性蛋白酶水解豆粕制备大豆多肽()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第6期
页码:
674-677
栏目:
出版日期:
2013-11-20

文章信息/Info

Title:
Production of Soybean Peptide by Alkaline Protease Hydrolysis of Soybean Meal
文章编号:
1000-5013(2013)06-0674-04
作者:
黄雅燕;  王文平;  肖美添
华侨大学 化工学院, 福建 厦门 361021
Author(s):
HUANG Ya-yan;  WANG Wen-ping;  XIAO Mei-tian
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
豆粕;  大豆肽;  碱性蛋白酶;  转化率;  水解度
Keywords:
soybean meal;  soybean peptide;  alkaline protease;  conversion rate;  degree of hydrolysis
分类号:
Q516
DOI:
10.11830/ISSN.1000-5013.2013.06.0674
文献标志码:
A
摘要:
采用碱性蛋白酶水解豆粕制备大豆多肽,以蛋白质的转化率和水解度为指标,通过单因素试验,考察影响酶解工艺的主要因素,并利用正交试验确定最佳酶解条件.结果表明:在温度为55 ℃,pH值为9.5,加酶量为0.15%,底物质量分数为7%的最佳条件下,水解豆粕3 h,其转化率可达75.73%,水解度为49.30%.液相色谱分析表明:酶解液的大豆多肽相对分子质量分布主要集中在5 000以下,占比达80.21%.
Abstract:
The soybean meal was hydrolyzed to prepare soy peptide with alkaline protease. During the experiment, the conversion rate and degree of hydrolysis were selected as the main indexes to investigate the chief factors influencing enzymolysis technology with single-factor experiment, then the optimum conditions were studied using orthogonal test. It was shown that the optimum conditions of enzymatic hydrolysis were temperature 55 ℃, pH 9.5, protease concentration 0.15%, substrate concentration 7%. Under the optimum conditions, the conversion rate and degree of hydrolysis were 75.73% and 59.30% respectively when hydrolysis for 3 h. The relative molecular weight distribution of soybean peptides was determined by size exclusion chromatography. Result showed that there was 80.21% of soybean peptide with molecular weight less than 5 000.

参考文献/References:

[1] 张亚丽,徐忠.变性脱脂豆粕酶解物的特性研究[J].中国食品学报,2005,5(4):46-51.
[2] 范宝庆,陈中.酸溶性酶解大豆蛋白的研究[J].广州食品工业科技,2004,20(3):24-25.
[3] MOLINA O S E,ANÓN M C.Analysis of products, mechanisms of reaction, and some functional properties of soy protein hydrolysates[J].J Am Oil Chem Soc,2002,77(12):1293-1301.
[4] 张毅.酶法制备大豆肽工艺条件的研究[D].江苏:江南大学,2009:17.
[5] 屠春燕,袁艳娟,徐娟,等. Alcalase碱性蛋白酶酶解绿豆分离蛋白制备小分子肽的工艺研究[J].食品研究与开发,2009,30(2):23-27.
[6] 陈钧辉,李俊,张太平,等.生物化学实验[M].4版.北京:科学出版社,2008:66-67.
[7] 夏宁,高媛,迟玉杰.蛋白酶水解低温豆粕制备大豆寡肽的研究[J].食品工业科技,2008,29(1):171-172.
[8] 余勃,陆兆新.微生物发酵法产大豆多肽液水解度的测定[J].食品科学,2005,26(4):104-107.
[9] 丁丹华,彭光华,何东平.HPLC法测定油茶籽多肽相对分子质量分布及氨基酸组成[J].中国油脂,2010,35(11):68-71.

备注/Memo

备注/Memo:
收稿日期: 2012-10-10
通信作者: 黄雅燕(1979-),女, 讲师,主要从事生物转化和生物合成的研究.E-mail:yyhuang@hqu.edu.cn.
基金项目: 福建省泉州市科技计划项目(2010N11)
更新日期/Last Update: 2013-11-20