[1]孙艳凤,孙向英,杨传孝.基于DNA双链增强量子点自组装膜能量转移的界面比率传感[J].华侨大学学报(自然科学版),2013,34(5):529-533.[doi:10.11830/ISSN.1000-5013.2013.05.0529]
 SUN Yan-feng,SUN Xiang-ying,YANG Chuan-xiao.Interfacial Ratio Sensing Based on DNA Double Helix Enhancing Energy Transfer in Quantum Dots Self-Assembled Multilayers[J].Journal of Huaqiao University(Natural Science),2013,34(5):529-533.[doi:10.11830/ISSN.1000-5013.2013.05.0529]
点击复制

基于DNA双链增强量子点自组装膜能量转移的界面比率传感()
分享到:

《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第5期
页码:
529-533
栏目:
出版日期:
2013-09-20

文章信息/Info

Title:
Interfacial Ratio Sensing Based on DNA Double Helix Enhancing Energy Transfer in Quantum Dots Self-Assembled Multilayers
文章编号:
1000-5013(2013)05-0529-05
作者:
孙艳凤 孙向英 杨传孝
华侨大学 材料科学与工程学院, 福建 厦门 361021
Author(s):
SUN Yan-feng SUN Xiang-ying YANG Chuan-xiao
College of Material Science and Engineering, Huaqiao University, Xiamen 361021, China
关键词:
能量转移 自组装膜 界面荧光 比率传感 脱氧核糖核酸
Keywords:
energy transfer self-assembled layers inferfacial fluorescence ratio sensing deoxyribonucleic acid
分类号:
O657.3
DOI:
10.11830/ISSN.1000-5013.2013.05.0529
文献标志码:
A
摘要:
分别以巯基乙酸(TGA)和三聚磷酸钠(STPP)为稳定剂,在水相条件下制备CdTe量子点和ZnO@CdS量子点.基于DNA双链增强自组装膜能量转移的原理,以ZnO@CdS量子点为能量供体,CdTe量子点为能量受体,构筑Quartz/PDDA/CdTe/PDDA/T1-DNA/PDDA/ZnO@CdS SAMs自组装膜,并实现对DNA的界面比率传感.随着匹配DNA(P1-DNA)的加入,ZnO@CdS量子点的荧光强度FD减小,相对荧光强度FA/FD和P1-DNA浓度的负对数呈线性关系,线性范围为8.686×10-9~6.080×10-8 mol·L-1,检测限为0.707 nmol·L-1.
Abstract:
CdTe quantum dots(QDs)and ZnO@CdS QDs were synthesized directly in aqueous solution which were respectively stabilized by thioglycolic acid and sodium tripolyphosphate. With ZnO@CdS quantum dots as energy donors and CdTe quantum dots as energy acceptor, Quartz/PDDA/CdTe/PDDA/T1-DNA/PDDA /ZnO@CdS SAMs was constructed and detected DNA by inferfacial ratio sensing successfully based on DNA double helix enhancing energy transfer of quantum dots self-assembled multilayers. With the matching DNA(P1-DNA)adding,the fluorescence intensity of ZnO@CdS QDs decreased and CdTe QDs increased. A good linear relationship between the relative fluorescence intensity FA/FD and the negative logarithm of concentration of P1-DNA could be obtained in the range from 8.686×10-9 to 6.080×10-8 mol·L-1 and the detection limit was 0.707 nmol·L-1.

参考文献/References:

[1] CHI Chun-wei,LAO Y H,LI Yi-shan,et al.A quantum dot-aptamer beacon using a DNA intercalating dye as the FRET reporter: Application to label-free thrombin detection[J].Biosensors and Bioelectronics,2011,26(7):3346-3352.
[2] WANG T H.Discerning single molecule interactions of DNA and quantum dots[J].Biotechnology Journal,2013,8(1):15-16.
[3] ASTAKHOVA I K, KUNAR T S, CAMLBELL M A, et al. Branched DNA nanostructures efficiently stabilized and monitored by novel pyrene-perylene 2’-α-L-amino-LNA FRET pairs[J].Chemical Communications,2013,49(5):511-513.
[4] SUZUKI M,HUSIMI Y,KOMATSU H,et al.Quantum dot FRET biosensors that respond to pH, to proteolytic or nucleolytic cleavage, to DNA synthesis or to a multiplexing combination[J].Journal of the American Chemical Society,2008,130(17):5720-5725.
[5] JIANG G,SUSHA A S,LUTICH A A,et al.Cascaded FRET in conjugated polymer/quantum dot/dye-labeled DNA complexes for DNA hybridization detection[J].ACS Properties,2009,3(12):4127-4131.
[6] SUN Xiang-ying,LIU Bin,ZHANG Ying.Rhodamine B aggregation in self-assembled multilayers induced by polyelectrolyte and interfacial fluorescence recognition for DNA[J].Talanta,2011,85(2):1187-1192.
[7] ZHANG Hao,ZHOU Zhen,YANG Bai.The influence of carboxyl groups on the photoluminescence of mercaptocarboxylic acid-stabilized CdTe nanoparticles[J].The Journal of Physical Chemistry B,2003,107(1):8-13.
[8] ZHANG Hao,WANG Li-ping,XIONG Huan-ming,et al.Hydrothermal synthesis for high-quality CdTe nanocrystals[J].Advanced Materials,2003,15(20):1712-1715.
[9] YANG Chuan-xiao,SUN Xiang-ying,LIU Bin.Visual and trap emission spectrometric detections of Ag(Ⅰ)ion with mesoporous ZnO/CdS@SiO2 core/shell nanocomposites[J].Analytica Chimica Acta,2012,746(9):90-97.
[10] SCHOLES G D.Long-range resonance energy transfer in molecular systems[J].Annual review of physical chemistry,2003,54(1):57-87.
[11] MARRAS S A E,KRAMER F R,TYAGI S.Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes[J].Nucleic Acids Research,2002,30(21):e122.
[12] HANNESTAD J K,SANDIN P,ALBINSSON B.Self-assembled DNA photonic wire for long-range energy transfer[J].Journal of the American Chemical Society,2008,130(47):15889.
[13] LEE L,JOHNSTON A P R,CARUSO F.Probing the dynamic nature of DNA multilayer films using Förster resonance energy transfer[J].Langmuir,2012,28(34):12527-12535.

相似文献/References:

[1]翁文婷,孙向英.Cu2+配位桥联作用组建荧光性多层膜[J].华侨大学学报(自然科学版),2005,26(3):255.[doi:10.3969/j.issn.1000-5013.2005.03.008]
 Weng Wenting,Sun Xiangying.Preparing a Multilayer Film with Fluorescence by Coordinate Bridging Action of Cu2+[J].Journal of Huaqiao University(Natural Science),2005,26(5):255.[doi:10.3969/j.issn.1000-5013.2005.03.008]
[2]连惠婷,孙向英,刘斌,等.二苯基硫脲自组装膜电极的制备及其特性[J].华侨大学学报(自然科学版),2006,27(4):372.[doi:10.3969/j.issn.1000-5013.2006.04.010]
 Lian Huiting,Sun Xiangying,Liu Bin,et al.The Study on Preparation and Characteristics of Diphenylthiourea Self-Assembled Monolayer Electrode[J].Journal of Huaqiao University(Natural Science),2006,27(5):372.[doi:10.3969/j.issn.1000-5013.2006.04.010]
[3]何燕芳,孙向英,刘斌.检测色氨酸的免标记生物传感器研制[J].华侨大学学报(自然科学版),2007,28(4):414.[doi:10.3969/j.issn.1000-5013.2007.04.021]
 HE Yan-fang,SUN Xiang-ying,LIU Bin.A Novel L-Tryptophan Sensor Based on Electrochemical Impedance[J].Journal of Huaqiao University(Natural Science),2007,28(5):414.[doi:10.3969/j.issn.1000-5013.2007.04.021]
[4]夏开豪,孙向英,刘斌,等.β-环糊精荧光增敏的间苯二酚荧光分析法[J].华侨大学学报(自然科学版),2008,29(4):550.[doi:10.11830/ISSN.1000-5013.2008.04.0550]
 XIA Kai-hao,SUN Xiang-ying,LIU Bin,et al.Detection of Resorcinol by Fluorescence Enhance of β-Cyclodextrin[J].Journal of Huaqiao University(Natural Science),2008,29(5):550.[doi:10.11830/ISSN.1000-5013.2008.04.0550]
[5]刘辉,孙向英,杨燕花,等.荧光性CdTe量子点自组装膜的合成及对Cu2+的界面传感[J].华侨大学学报(自然科学版),2009,30(5):533.[doi:10.11830/ISSN.1000-5013.2009.05.0533]
 LIU Hui,SUN Xiang-ying,YANG Yan-hua,et al.Synthesis of CdTe Quantum Dots and Interfacial Sensing for Cu2+ Based on Self-Assembled Monolayers[J].Journal of Huaqiao University(Natural Science),2009,30(5):533.[doi:10.11830/ISSN.1000-5013.2009.05.0533]
[6]杨光昕,孙向英,刘斌.荧光探针自组装膜对久效磷农药的检测[J].华侨大学学报(自然科学版),2010,31(1):37.[doi:10.11830/ISSN.1000-5013.2010.01.0037]
 YANG Guang-xin,SUN Xiang-ying,LIU Bin.Determination of Monocrotophos Based on Self-Assembled Multilayer Films with Fluorescent Probes[J].Journal of Huaqiao University(Natural Science),2010,31(5):37.[doi:10.11830/ISSN.1000-5013.2010.01.0037]
[7]柯培林,孙向英.CdS量子点自组装膜对DNA的界面传感[J].华侨大学学报(自然科学版),2011,32(1):48.[doi:10.11830/ISSN.1000-5013.2011.01.0048]
 KE Pei-lin,SUN Xiang-ying.Prepariton of CdS Self-Assembled Monolayers and Interfacial Sensing for DNA[J].Journal of Huaqiao University(Natural Science),2011,32(5):48.[doi:10.11830/ISSN.1000-5013.2011.01.0048]

备注/Memo

备注/Memo:
收稿日期: 2013-04-27
通信作者: 孙向英(1965-),女,教授,主要从事纳米材料自组装与传感的研究.E-mail:sunxy@hqu.edu.cn.
基金项目: 国家自然科学基金资助项目(21275059, 21175049); 福建省自然科学基金资助项目(2011J01049, 2012J01044); 中央高校基本科研业务费专项基金资助项目(JB-2R1118)
更新日期/Last Update: 2013-09-20