[1]王镇发,陈培钦,邓秩韬,等.反胶束萃取阿魏酸酯酶[J].华侨大学学报(自然科学版),2013,34(2):182-185.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
 WANG Zhen-fa,CHEN Pei-qin,DENG Yi-tao,et al.Purification of Feruloyl Esterase Using Reverse Micelles Extraction[J].Journal of Huaqiao University(Natural Science),2013,34(2):182-185.[doi:10.11830/ISSN.1000-5013.2013.02.0182]
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反胶束萃取阿魏酸酯酶()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第34卷
期数:
2013年第2期
页码:
182-185
栏目:
出版日期:
2013-03-20

文章信息/Info

Title:
Purification of Feruloyl Esterase Using Reverse Micelles Extraction
文章编号:
1000-5013(2013)02-0182-04
作者:
王镇发 陈培钦 邓秩韬 谢晨 李夏兰
华侨大学 化工学院, 福建 厦门 361021
Author(s):
WANG Zhen-fa CHEN Pei-qin DENG Yi-tao XIE Chen LI Xia-lan
College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
关键词:
阿魏酸酯酶 反胶束 萃取 十六烷基三甲基溴化铵
Keywords:
feruloyl esterase reverse micelles extraction cetrimonium bromide
分类号:
Q556.1
DOI:
10.11830/ISSN.1000-5013.2013.02.0182
文献标志码:
A
摘要:
利用十六烷基三甲基溴化铵(CTAB)/正己醇/异辛烷反胶束萃取阿魏酸酯酶.考察pH值、离子强度、CTAB浓度对阿魏酸酯酶的CTAB/正己醇/异辛烷反胶束萃取及其反萃取的影响.结果表明:萃取的最佳条件为酶液透析24 h,pH值为12,萃取液为25 mmol·L-1 CTAB的正己醇/异辛烷溶液(体积比为1∶5),萃取率接近100%;反萃取的最佳条件为pH值为7的0.20 mol·L-1 KCl溶液,反萃取率可达79%.纯化后比活为356.7 μkat·g-1,纯化倍数为6.9,SDS-PAGE电泳显示两条带.
Abstract:
The cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were used to purify feruloyl esterase. The effects of pH, ionic strength and cetrimonium bromide(CTAB)concentration on the cetrimonium bromide/hexanol/isooctane reverse micelles extraction and backward extraction were studied. The results showed that the extraction efficiency was nearly 100% when the optimal extraction conditions were used as followed: enzyme dialysis time 24 h, pH 12 and CTAB concentration in hexanol/isooctane solution(volume ratio 1∶5)25 mmol·L-1. The optimal condition of backward extraction was 0.20 mol·L-1 KCl with pH 7, and the efficiency of backward extraction was 79%. After extraction and backward extraction of the enzyme, specific activity was 356.7 μkat·g-1, purification fold was 6.9, and SDS-PAGE electrophoresis showed two bands.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期: 2012-04-18
通信作者: 李夏兰(1965-),女,教授,主要从事生物化工的研究.E-mail:xialan@hqu.edu.cn.
基金项目: 福建省科技计划重点基金资助项目(2011N0020); 华侨大学科研基金资助项目(09X0289); 华侨大学高层次人才启动基金资助项目(11BS221)
更新日期/Last Update: 2013-03-20