[1]施腾鑫,刘嘉,贺淹才.黏质沙雷氏菌产几丁质酶的发酵工艺优化[J].华侨大学学报(自然科学版),2010,31(6):667-670.[doi:10.11830/ISSN.1000-5013.2010.06.0667]
　SHI Teng-xin,LIU Jia,HE Yan-cai.Culture Parameter Optimization of Chitinase Production by Serratia marcescens[J].Journal of Huaqiao University(Natural Science),2010,31(6):667-670.[doi:10.11830/ISSN.1000-5013.2010.06.0667]
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黏质沙雷氏菌产几丁质酶的发酵工艺优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第31卷
期数:
2010年第6期
页码:
667-670
栏目:
出版日期:
2010-11-20

文章信息/Info

Title:
Culture Parameter Optimization of Chitinase Production by Serratia marcescens
文章编号:
1000-5013(2010)06-0667-04
作者:
施腾鑫; 刘嘉; 贺淹才
华侨大学化工学院
Author(s):
SHI Teng-xin;  LIU Jia;  HE Yan-cai
College of Chemical Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
黏质沙雷氏菌;  几丁质酶;  均匀设计;  正交设计
Keywords:
Serratia marcescens;  chitinase;  uniform-design;  orthogonal-design
分类号:
TQ925
DOI:
10.11830/ISSN.1000-5013.2010.06.0667
文献标志码:
A
摘要:
利用均匀设计法,优化一株黏质沙雷氏菌(Serratia marcescens)产几丁质酶培养基的组分; 同时,利用正交设计法优化其摇瓶发酵产酶的条件.研究结果表明,最佳培养基组分(质量分数):0.504%胶体几丁质,1.178%酵母粉,0.025%MgSO4.7H2O,0.095%K2HPO4,0.001%FeSO4.7H2O,0.005%山梨醇,0.030%KH2PO4; 最佳摇瓶发酵工艺条件:培养时间为48 h,发酵温度为30℃,初始pH值为9.0,装液量为30 mL,接种量为1%.在此优化条件下,酶活力可达9.39μkat.L-1,比未优化的产酶条件下酶活力提高了81.6%.
Abstract:
Chitinase-producing Serratia marcescens culture medium components and culture conditions of enzyme production by shake flask fermentation were optimized with uniform-design and orthogonal-design respectively.The results showed that the optimal component concentrations(m/m) were colloid chitin 0.504%,yeast extract 1.178%,MgSO4·7H2O 0.025%,K2HPO4 0.095%,FeSO4·7H2O 0.001%,sorbitol 0.005%,KH2PO4 0.030%.The optimal culture conditions were incubation time 48 h,temperature 30 ℃,initial pH 9.0,loadage 30 mL per 250 mL flask,inoculation quantity 1%.The activity of chitinase reached 9.39 μkat·L-1 which was increased by 81.6% than that of the unoptimized conditions..

参考文献/References:

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备注/Memo

备注/Memo:
福建省自然科学基金资助项目(C04010011)
更新日期/Last Update: 2014-03-23