[1]陈巍,林锡煌,戴丹凤,等.短乳杆菌产NADH氧化酶的发酵及纯化[J].华侨大学学报(自然科学版),2010,31(5):548-551.[doi:10.11830/ISSN.1000-5013.2010.05.0548]
　CHEN Wei,LIN Xi-huang,DAI Dan-feng,et al.Fermentation and Purification of NADH Oxidase from Lactobacillus brevis[J].Journal of Huaqiao University(Natural Science),2010,31(5):548-551.[doi:10.11830/ISSN.1000-5013.2010.05.0548]
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短乳杆菌产NADH氧化酶的发酵及纯化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第31卷
期数:
2010年第5期
页码:
548-551
栏目:
出版日期:
2010-09-20

文章信息/Info

Title:
Fermentation and Purification of NADH Oxidase from Lactobacillus brevis
文章编号:
1000-5013(2010)05-0548-04
作者:
陈巍; 林锡煌; 戴丹凤; 方柏山
华侨大学工业生物技术福建省高等学校重点实验室
Author(s):
CHEN Wei;  LIN Xi-huang;  DAI Dan-feng;  FANG Bai-shan
Key Laboratory of Industrial Biotechnology Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
NADH氧化酶;  短乳杆菌;  发酵;  纯化
Keywords:
NADH oxidase;  Lactobacillus brevis;  fermentation;  purification
分类号:
Q814
DOI:
10.11830/ISSN.1000-5013.2010.05.0548
文献标志码:
A
摘要:
有氧环境下,利用优化后的发酵培养基,研究短乳杆菌(Lactobacillus brevis)产NADH氧化酶(NOX)的发酵条件.采用8层纱布,在温度为30℃,pH=7.0,转速为150 r.min-1的条件下发酵培养23 h,用pH值为5.8的0.1 mol.L-1磷酸钾缓冲液对细胞进行洗涤悬浮,超声波破碎(功率为400 W,超声2 s,间歇2 s,破碎12 min)后,测得其比活力可达2.317 mkat.g-1.通过DEAE Sepharose Fast Flow,Macro-Prep High Q和Hydroxyapatite-Ⅰ三步纯化后,其纯化倍数为3.11,回收率为8.50%.
Abstract:
Researches on fermentation conditions of NADH oxidase(NOX) from Lactobacillus brevis were studied in aerobic environment using optimal fermentation medium.Eight layer gauze was used and the Lactobacillus brevis was cultured under 30 ℃ for 23 h,with pH 7.0 and rotation speed 150 r·min-1.Then the cells were washed and suspended with 0.1 mol·L-1 K-PBS and disintegrated for 12 min using an ultrasonic cell disintegrator under 400 W(pulse 2 s,interval 2 s).The final enzymatic activity obtained was 2.371 mkat·g-1.In the end,the NOX was purified by DEAE Sepharose Fast Flow,Macro-Prep High Q and Hydroxyapatite-Ⅰ,a 3.11 purification fold was obtained with the recovery enzymatic activity of 8.5%.

参考文献/References:

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相似文献/References:

[1]卿三红,方柏山.NADH氧化酶的研究进展[J].华侨大学学报(自然科学版),2011,32(5):554.[doi:10.11830/ISSN.1000-5013.2011.05.0554]
　QING San-hong,FANG Bai-shan.Recent Progress in Research of NADH Oxidase[J].Journal of Huaqiao University(Natural Science),2011,32(5):554.[doi:10.11830/ISSN.1000-5013.2011.05.0554]

备注/Memo

备注/Memo:
国家高技术研究发展(863)计划项目(2006AA020103); 国家自然科学基金资助项目(20676048)
更新日期/Last Update: 2014-03-23