[1]范韵敏,李夏兰,方柏山.阿魏酸酯酶产生菌的培养条件优化[J].华侨大学学报(自然科学版),2010,31(4):426-429.[doi:10.11830/ISSN.1000-5013.2010.04.0426]
　FAN Yun-min,LI Xia-lan,FANG Bai-shan.Optimization of Fermentation Conditions of Ferulic Acid Esterase Producers[J].Journal of Huaqiao University(Natural Science),2010,31(4):426-429.[doi:10.11830/ISSN.1000-5013.2010.04.0426]
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阿魏酸酯酶产生菌的培养条件优化()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第31卷
期数:
2010年第4期
页码:
426-429
栏目:
出版日期:
2010-07-20

文章信息/Info

Title:
Optimization of Fermentation Conditions of Ferulic Acid Esterase Producers
文章编号:
1000-5013(2010)04-0426-04
作者:
范韵敏; 李夏兰; 方柏山
华侨大学工业生物技术福建省高校重点实验室
Author(s):
FAN Yun-min;  LI Xia-lan;  FANG Bai-shan
Key Laboratory of Industrial Biotechnology of Fujian Province, Huaqiao University, Quanzhou 362021, China
关键词:
阿魏酸酯酶;  麦糟;  优化;  均匀设计
Keywords:
ferulic acid esterase;  brewer’s spent grain;  optimization;  uniform-design
分类号:
TQ920.6
DOI:
10.11830/ISSN.1000-5013.2010.04.0426
文献标志码:
A
摘要:
通过均匀设计法和二次多项式逐步回归,对产阿魏酸酯酶的培养基配方进行优化,得出优化的培养基配方(质量分数):0.30%KH2PO4,0.60%Na2HPO4.7H2O,0.01%NaCl,0.80%酵母粉,1.33%麦糟.在此基础上,用单因素法考察发酵条件对产酶的影响,确定最佳条件:初始pH值为6.0,培养温度为28℃,摇床转速为210 r.min-1,发酵时间为68 h,装液量为75 mL,接种量为5%,麦糟粒径为0.054 mm.采用优化的培养基组分进行最优发酵培养,优化后的阿魏酸酯酶酶活达到119.36μkat.L-1,比优化前提高了32倍.
Abstract:
The optimal parameters of fermentation conditions of ferulic acid esterase from brewer’s spent grain were obtained through uniform-design and quadratic polynomial regression techniques.The optimal culture medium were as follows(%):KH2PO4 0.30,Na2HPO4·7H2O 0.60,NaCl 0.01,yeast powder 0.80,brewer’s spent grain 1.33.Based on the results above,the influence of fermentation conditions on enzymes were also studied by single factor method and the optimal conditions were confirmed as follows: the optimization initial pH 6.0,cultural temperature 28 ℃,the strain was cultivated with 75 mL medium in a 250 mL flask for 68 h on 210 r·min-1 shaker,inoculum concentration 5% and grain diameter 0.054 mm.Ferulic acid esterase reached 119.36 μkat·L-1 under optimal conditions,increased 32 times than that before optimization.

参考文献/References:

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备注/Memo

备注/Memo:
国家重点基础研究发展(973)计划项目(2007CB707804)
更新日期/Last Update: 2014-03-23