[1]张兰,程珊影,李夏兰,等.高效液相色谱测定麦糟水解液中单糖和有机酸[J].华侨大学学报(自然科学版),2010,31(2):187-191.[doi:10.11830/ISSN.1000-5013.2010.02.0187]
　ZHANG Lan,CHENG Shan-ying,LI Xia-lan,et al.Analysis of Monosaccharides and Organic Acids in the Liquors of Acid Hydrolysis on Brewery’s Spent Grain by HPLC[J].Journal of Huaqiao University(Natural Science),2010,31(2):187-191.[doi:10.11830/ISSN.1000-5013.2010.02.0187]
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高效液相色谱测定麦糟水解液中单糖和有机酸()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第31卷
期数:
2010年第2期
页码:
187-191
栏目:
出版日期:
2010-03-20

文章信息/Info

Title:
Analysis of Monosaccharides and Organic Acids in the Liquors of Acid Hydrolysis on Brewery’s Spent Grain by HPLC
文章编号:
1000-5013(2010)02-0187-05
作者:
张兰; 程珊影; 李夏兰; 方柏山
华侨大学化工学院
Author(s):
ZHANG Lan;  CHENG Shan-ying;  LI Xia-lan;  FANG Bai-shan
College of Chemical Engineering, Huaqiao University, Quanzhou, 362021, China
关键词:
麦糟;  单糖;  有机酸;  糖醛酸;  高效液相色谱
Keywords:
brewery’s spent grain;  monosaccharide;  organic acid;  uronic acid;  high performance liquid chromatography
分类号:
TQ353.14
DOI:
10.11830/ISSN.1000-5013.2010.02.0187
文献标志码:
A
摘要:
建立在麦糟水解液中,单糖、有机酸和糖醛酸等的高效液相色谱测定法.在确定的色谱条件下,葡萄糖、阿拉伯糖、鼠李糖、甲酸、乙酸、葡萄糖醛酸、半乳糖醛酸的分离状况良好,其峰面积与质量浓度的线性关系良好,相关系数R2值为0.991 2~0.999 7,回收率为99.40%~105.90%.木糖、半乳糖、甘露糖在AminexHPX-87H柱上不能分离,但可用ZORBAX Carbohydrate Analysis柱分离.采用示差折光检测器检测,柱温为30℃,流动相中乙腈和水的体积比为80∶20,流速为1.0 mL.min-1,进样量为0.02 mL,外标法定量,结果表明,其峰面积与浓度线性关系良好,相关系数R2值为0.995 9~0.999 1,回收率为99.99%~102.70%.
Abstract:
High performance liquid chromatography(HPLC) method was constructed to test individual monosaccharides and organic acids in the liquors of acid hydrolysis on brewery’s spent grain.Mixture of the glucose,arabinose,rhamnose,formic acid,acetic acid,glucuronic acid and galacturonic acid was measured in the given conditions.The correlation coefficient of these matters was 0.991 2 to 0.999 7 and the corresponding average recovery was 99.40% to 105.90%.For the case where,xylose,galactose and mannose can not be separated by Aminex HPX-87H column,ZORBAX Carbohydrate Analysis column was used for further separation.The operating conditions were as follows: ZORBAX Carbohydrate Analysis column with differential refractive index detector,30 ℃,elution influent acetonitrile∶water(80∶20; volume ratio),flow rate 1.0 mL·min-1 and injection volume 0.02 mL.Under these conditions,the correlation coefficient was 0.995 9 to 0.999 1 and the corresponding average recovery was 99.99% to 102.70%.

参考文献/References:

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[3]余晓芬,刘英丽,程珊影,等.麦糟水解液中单糖及脂肪酸的HPLC分析[J].华侨大学学报(自然科学版),2007,28(4):403.[doi:10.3969/j.issn.1000-5013.2007.04.018]
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备注/Memo

备注/Memo:
国家重点基础研究发展(973)计划项目(2007CB707804)
更新日期/Last Update: 2014-03-23