[1]罗菊香,王晓霞,方柏山,等.重组PDOR基因的表达与纯化及部分酶学性质[J].华侨大学学报(自然科学版),2009,30(2):166-170.[doi:10.11830/ISSN.1000-5013.2009.02.0166]
 LUO Ju-xiang,WANG Xiao-xia,FANG Bai-shan,et al.Expression,Purification and Characterization of 1,3-Propanediol Oxidoreductase from Klebsiella pneumoniae[J].Journal of Huaqiao University(Natural Science),2009,30(2):166-170.[doi:10.11830/ISSN.1000-5013.2009.02.0166]
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重组PDOR基因的表达与纯化及部分酶学性质()
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《华侨大学学报(自然科学版)》[ISSN:1000-5013/CN:35-1079/N]

卷:
第30卷
期数:
2009年第2期
页码:
166-170
栏目:
出版日期:
2009-03-20

文章信息/Info

Title:
Expression,Purification and Characterization of 1,3-Propanediol Oxidoreductase from Klebsiella pneumoniae
文章编号:
1000-5013(2009)02-0166-05
作者:
罗菊香王晓霞方柏山夏启容
华侨大学工业生物技术福建省高等学校重点实验室
Author(s):
LUO Ju-xiang WANG Xiao-xia FANG Bai-shan XIA Qi-rong
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
13-丙二醇氧化还原酶 基因表达 纯化 酶学性质
Keywords:
13-propanediol oxidoreductase expression purification characterisation
分类号:
Q789
DOI:
10.11830/ISSN.1000-5013.2009.02.0166
文献标志码:
A
摘要:
将dhaT在E.coliBL21(DE3)pLysS中进行表达,并对含有His6标记的1,3-丙二醇氧化还原酶(PDOR)进行纯化.重组PDOR反应的最适pH值为10.0,最适温度为55℃,其以1,3-丙二醇和NAD+为底物的表观米氏常数Km值分别是15.5,0.23 mmol.L-1.实验表明,Ca2+,Mg2+和Cu2+对酶活性有抑制作用,而Fe2+,Na+,NH4+和Mn2+对酶活性有促进作用; 1,3-丙二醇是PDOR适合的氧化底物.
Abstract:
The dhaT gene was expressed with protein in E.coli BL21(DE3)pLysS and the His6-tagged 1,3-propanediol oxidoreductase(PDOR) was purified.Optimal pH and temperature for purified enzyme were 10.0 and 55 ℃,respectively.The apparent Km values of the enzyme for 1,3-propanediol and NAD+ were 13.5 and 0.25 mmol·L-1,respectively.The enzyme could be inhibited by metal ions including Ca2+,Mg2+ and Cu2+,while increased by Fe2+,Na+,NH4+ and Mn2+; PDOR showed high specificity for the alcohol substrate.

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备注/Memo

备注/Memo:
国家重点基础研究发展(973)计划项目(2007CB707804); 国家自然科学基金资助项目(20446004,20676048); 福建省科技计划重点项目(2003I020)
更新日期/Last Update: 2014-03-23