[1]石敢当,张光亚,方柏山.新型G/11家族木聚糖酶的设计及生物学分析[J].华侨大学学报(自然科学版),2008,29(4):559-562.[doi:10.11830/ISSN.1000-5013.2008.04.0559]
　SHI Gan-dang,ZHANG Guang-ya,FANG Bai-shan.Design and Bioinformatical Analysis of a Novel G/11 Xylanase[J].Journal of Huaqiao University(Natural Science),2008,29(4):559-562.[doi:10.11830/ISSN.1000-5013.2008.04.0559]
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新型G/11家族木聚糖酶的设计及生物学分析()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第4期
页码:
559-562
栏目:
出版日期:
2008-10-20

文章信息/Info

Title:
Design and Bioinformatical Analysis of a Novel G/11 Xylanase
文章编号:
1000-5013(2008)04-0559-04
作者:
石敢当; 张光亚; 方柏山
华侨大学工业生物技术重点实验室
Author(s):
SHI Gan-dang;  ZHANG Guang-ya;  FANG Bai-shan
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
木聚糖酶;  调和序列;  三级结构;  理化性质;  合成生物学
Keywords:
xylanase;  consensus sequence;  tertiary structure;  physical and chemical properties;  synthetic biology
分类号:
Q814
DOI:
10.11830/ISSN.1000-5013.2008.04.0559
文献标志码:
A
摘要:
运用Bioedit软件,从已知耐热性能较好的G/11木聚糖酶蛋白序列出发,构建调和序列,获得新型木聚糖酶.首先运用InterProScan得出该序列为G/11家族,再运用MotifScan确定其活性部位,对比G/11木聚糖酶的特征序列确定该酶为木聚糖酶.通过SWISS-MODEL对其三级结构进行预测,了解信号肽长度为27个.运用Protparam Tool对其部分理化性质进行预测,得出其理论等电点为6.82,最适温度为81.72℃,高于调和前的任何一条序列.最后,通过选择宿主菌最优密码子获得其基因序列.
Abstract:
A novel xylanase sequence was obtained by using Bioedit to compare six G/11 thermostable xylanase protein sequences.The calculated optimal temperature of the new sequence was 81.72 ℃,which was higher than any of the six ancestral sequences.Firstly,we knew this new sequence belonged to G/11 through using InterProScan,and got its active site by using MotifScan.The signal peptide of the new xylanase could know by using the SWISS-MODEL which predicted the tertiary structure of the protein.The length of the signal peptide was 27.By using protparam tool,we got some physical and chemical properties of the new xylanase,from which we knew the theoretical isoelectric point of it was 6.28.The gene encoding of the xylanase was generated through the optimal codon used by the host strain.The current study paved the way for the synthetic biology of xylanase.

参考文献/References:

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备注/Memo

备注/Memo:
国家重点基础研究发展(973)计划项目(2007CB707804); 国家高技术研究发展(863)计划项目(2006AA020103); 国家自然科学基金资助项目(20676048)
更新日期/Last Update: 2014-03-23