[1]王晓霞,方柏山,李雯君.木糖还原酶基因在大肠杆菌中活性表达[J].华侨大学学报(自然科学版),2008,29(3):383-386.[doi:10.11830/ISSN.1000-5013.2008.03.0383]
　WANG Xiao-xia,FANG Bai-shan,LI Wen-jun.Expression of Xylose Reductase Genes from Candida shehatae in E.coli[J].Journal of Huaqiao University(Natural Science),2008,29(3):383-386.[doi:10.11830/ISSN.1000-5013.2008.03.0383]
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木糖还原酶基因在大肠杆菌中活性表达()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第29卷
期数:
2008年第3期
页码:
383-386
栏目:
出版日期:
2008-07-20

文章信息/Info

Title:
Expression of Xylose Reductase Genes from Candida shehatae in E.coli
文章编号:
1000-5013(2008)03-0383-04
作者:
王晓霞; 方柏山; 李雯君
华侨大学工业生物技术福建省高等学校重点实验室; 华侨大学工业生物技术福建省高等学校重点实验室 福建泉州362021; 福建泉州362021
Author(s):
WANG Xiao-xia;  FANG Bai-shan;  LI Wen-jun
Key Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
木糖还原酶;  乳糖诱导;  聚合酶链式反应;  大肠杆菌;  活性表达
Keywords:
xylose reductase;  induct by lactose;  polymerase chain reaction;  Candida shehatae;  activity expression
分类号:
Q78
DOI:
10.11830/ISSN.1000-5013.2008.03.0383
文献标志码:
A
摘要:
利用聚合酶链式反应(PCR)方法,从休哈塔假丝酵母(Candida shehatae)基因组DNA扩增得到了木糖还原酶(Xylose Reductase,XR)基因,并在大肠杆菌中活性表达.重组菌在诱导6 h时的XR表达量最大,约为总蛋白的20%.实验考察不同质量浓度的乳糖和异丙基--βD-硫代半乳糖苷(IPTG)对酶活性的影响,结果表明,重组菌在以乳糖作为诱导剂时酶活最高为232.38μkat.g-1,以IPTG作为诱导剂时酶活最高为206.04μkat.g-1.
Abstract:
The xr gene encoding xylose reductase was amplified by using the genomic DNA of Candida shehatae CICC1766 as the template and expressed heterologously with protein in E.coli BL21(DE3) plysS.The expression level which accounted for 20% of total cell protein reached the maximum degree after 6 h of induction.And the effect of lactose and IPTG was investigated as the inducer on specific activity of XR.The maximum activity was 232.38 μkat·g-1 with lactose as the inducer,while with IPTG was 206.04 μkat·g-1.

参考文献/References:

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　Wan Ning,Li Weicheng,Fang Baishan,et al.Property and Influencing Factors of Catalysis Induced by Xylose Reductase in Crude Extract of Candida Mogii Cells[J].Journal of Huaqiao University(Natural Science),2001,22(3):194.[doi:10.3969/j.issn.1000-5013.2001.02.019]

备注/Memo

备注/Memo:
福建省自然科学重点基金资助项目(C96068)
更新日期/Last Update: 2014-03-23