[1]何燕芳,孙向英,刘斌.检测色氨酸的免标记生物传感器研制[J].华侨大学学报(自然科学版),2007,28(4):414-417.[doi:10.3969/j.issn.1000-5013.2007.04.021]
　HE Yan-fang,SUN Xiang-ying,LIU Bin.A Novel L-Tryptophan Sensor Based on Electrochemical Impedance[J].Journal of Huaqiao University(Natural Science),2007,28(4):414-417.[doi:10.3969/j.issn.1000-5013.2007.04.021]
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检测色氨酸的免标记生物传感器研制()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第28卷
期数:
2007年第4期
页码:
414-417
栏目:
出版日期:
2007-10-20

文章信息/Info

Title:
A Novel L-Tryptophan Sensor Based on Electrochemical Impedance
文章编号:
1000-5013(2007)04-0414-04
作者:
何燕芳; 孙向英; 刘斌
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021
Author(s):
HE Yan-fang;  SUN Xiang-ying;  LIU Bin
College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China
关键词:
生物传感器;  自组装膜;  电化学阻抗谱;  铜离子;  L-色氨酸
Keywords:
biosensor;  self-assembled membrane;  electrochemical impedance;  copper ion;  L-tryptophan
分类号:
TP212.3
DOI:
10.3969/j.issn.1000-5013.2007.04.021
文献标志码:
A
摘要:
利用Au-S化学键的作用,在金基底上组装L-半胱氨酸(L-Cys),通过铜离子的配位作用,构建Cu2+/L-Cys/Au自组装膜.考察该自组装膜的组装条件、电化学特征、重现性与稳定性,研究不同浓度的L-色氨酸中膜的识别能力和识别选择性.结果表明,自组装膜具有对L-色氨酸识别的选择性,在不同浓度的L-色氨酸中,膜的电化学阻抗会发生明显变化.因此,利用该膜对L-色氨酸(L-Try)的选择性识别引起电化学阻抗变化的特性,可构建一种新型的免标记电化学阻抗法色氨酸传感器.
Abstract:
With the help of chemical bonding of Au-S,L-cysteiney(L-Cys) was assembled onto the gold surface to form L-Cys/Au self-assembled monolayer.Taking advantage of coordinate action of Cu2+with L-cysteine,the Cu2+/L-Cys/Au self-assembled membrane was constructed.The films assembling conditions,electrochemical characteristics,reproducibility and stability are discussed,and the reorganization selectivity for L-tryptophan(L-Try) are also studied.The experimental results showed that the film Cu2+/L-Cys/Au could selectively recognize for L-tryptophan.When Cu2+/L-Cys/Au acted on L-tryptophan,the electrochemical impedance changed,and the self-assembled membrane could selectively recognize L-tryptophan.A novel L-tryptophan sensor based on electrochemical impedance was built up.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20575023); 福建省国际合作重点项目(2006I0021); 福建省自然科学基金资助项目(D0410019)
更新日期/Last Update: 2014-03-23