[1]兰琳,林锡煌,彭益强,等.耐高浓度甘油的1,3-丙二醇生产菌的诱变选育[J].华侨大学学报(自然科学版),2007,28(4):399-402.[doi:10.3969/j.issn.1000-5013.2007.04.017]
　LAN Lin,LIN Xi-huang,PENG Yi-qiang,et al.Breeding of Strain Producing 1,3-Propanediol in High Concentration of Glycerol[J].Journal of Huaqiao University(Natural Science),2007,28(4):399-402.[doi:10.3969/j.issn.1000-5013.2007.04.017]
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耐高浓度甘油的1,3-丙二醇生产菌的诱变选育()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第28卷
期数:
2007年第4期
页码:
399-402
栏目:
出版日期:
2007-10-20

文章信息/Info

Title:
Breeding of Strain Producing 1,3-Propanediol in High Concentration of Glycerol
文章编号:
1000-5013(2007)04-0399-04
作者:
兰琳; 林锡煌; 彭益强; 方柏山
华侨大学工业生物技术福建省高等学校重点实验室; 华侨大学工业生物技术福建省高等学校重点实验室 福建泉州362021; 福建泉州362021
Author(s):
LAN Lin;  LIN Xi-huang;  PENG Yi-qiang;  FANG Bai-shan
Laboratory of Industrial Biotechnology of Fujian Province University, Huaqiao University, Quanzhou 362021, China
关键词:
克雷伯杆菌;  1; 3-丙二醇;  紫外诱变;  甘油
Keywords:
Klebsiella pneumoniae;  1; 3-propanediol;  ultraviolet mutagenesis;  glycerol
分类号:
TQ223.162
DOI:
10.3969/j.issn.1000-5013.2007.04.017
文献标志码:
A
摘要:
利用紫外线对克雷伯杆菌进行诱变,筛选出耐高浓度甘油且1,3-丙二醇(1,3-PD)产量较高的突变株KpⅥ.实验结果表明,在距离34 cm,功率30 W的紫外灯垂直照射下,最佳紫外诱变时间为6 min,此时,致死率为90.9%.克雷伯杆菌经过紫外诱变后,菌落明显增大,是原始菌株的2~4倍; 变异菌株KpⅥ经过6代遗传稳定性考察,甘油消耗率和1,3-PD产量稳定,且保持了较高水平.在甘油质量浓度为90 g.L-1的条件下,变异菌株KpⅥ的甘油消耗率为98.3%,甘油转化率为50.4%,1,3-PD产量为44.81 g.L-1,生产能力达到0.75 g.(L.h)-1.
Abstract:
A mutant Klebsiella pneumoniae(KpⅥ) strain that endure high glycerol concentration and had high 1,3-PD yield was screened by ultraviolet(UV).The optimal mutation time was 6 min when 30 W ultraviolet plumb irradiation from 30 cm distance,the lethal ratio was 90.9%.The Klebsiella pneumoniae strain was enlarged distinctly after ultraviolet mutation,2~4 times than original strain.The mutant strain KpⅥ had showed steady glycerol consumption rate and high 1,3-PD yield via 6 generation inherit stability review.The mutant strain′s glycerol consumption rate was 98.3%,glycerol transformation rate was 50.4%,1,3-PD yield was 44.8 g·L-1 and the through put was 0.75 g·(L·h)-1 when glycerol concentration was 90 g·L-1.

参考文献/References:

[1] 刘德华, 刘宏娟, 程可可. 微生物发酵法生产1, 3-丙二醇研究进展 [J]. 合成纤维, 2005(9):11-15.doi:10.3969/j.issn.1001-7054.2005.09.003.
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[6] 赵红英, 张建, 刘宏娟. 1, 3-丙二醇发酵过程中底物抑制及其对策的研究 [J]. 现代化工, 2002(7):34-38.doi:10.3321/j.issn:0253-4320.2002.07.009.
[7] 李凤梅, 刘长江, 郑艳. 产1, 3-丙二醇菌株丁酸梭菌的诱变育种 [J]. 生物技术, 2005(1):34-35.doi:10.3969/j.issn.1004-311X.2005.01.015.
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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026,20446004); 福建省科技计划重点项目(2003I020)
更新日期/Last Update: 2014-03-23