[1]杨传孝,黄承志.TAPP与DNA作用的共振光散射光谱校正[J].华侨大学学报(自然科学版),2007,28(1):34-37.[doi:10.3969/j.issn.1000-5013.2007.01.010]
　YANG Chuan-xiao,Huang CHENG-zhi.Studies on the Interaction of α,β,γ,δ-Tetrakis [4-(Trimethylammoniumyl) Phenyl] Porphine with DNA by Corrections of Resonance Light Scattering Spectra[J].Journal of Huaqiao University(Natural Science),2007,28(1):34-37.[doi:10.3969/j.issn.1000-5013.2007.01.010]
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TAPP与DNA作用的共振光散射光谱校正()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第28卷
期数:
2007年第1期
页码:
34-37
栏目:
出版日期:
2007-01-20

文章信息/Info

Title:
Studies on the Interaction of α,β,γ,δ-Tetrakis [4-(Trimethylammoniumyl) Phenyl] Porphine with DNA by Corrections of Resonance Light Scattering Spectra
文章编号:
1000-5013(2007)01-0034-04
作者:
杨传孝; 黄承志
华侨大学材料科学与工程学院; 西南大学化学化工学院 福建泉州362011; 重庆400715
Author(s):
YANG Chuan-xiao1;  Huang CHENG-zhi2
1.College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China; 2.College of Chemistry and Engineering, Southwest University, Chongqing 400715, China
关键词:
共振光散射;  光谱校正;  α; β; γ; δ-四-(对三甲氨基苯基)卟啉;  小牛胸腺DNA(ctDNA)
Keywords:
resonance light scattering;  spectrum correction;  α; β; γ; δ-tetrakis(4-(trimethylammoniumyl) phenyl) porphine;  calf thymus DNA
分类号:
O657.3
DOI:
10.3969/j.issn.1000-5013.2007.01.010
文献标志码:
A
摘要:
用吸收装置改变荧光分光光度计入射光的光路,获得光源的强度光谱和样品的透光光谱,并在同一台仪器上建立一种简单的分离表观吸收光谱中吸收和散射成分的方法.通过对TAPP(α,β,γ,δ-四-(对三甲氨基苯基)卟啉)与DNA作用的共振光散射光谱进行校正,结果表明,校正表观分子吸收后,灵敏度提高了2倍左右.校正仪器影响后,从F-2500和F-4500荧光分光光度计获得表观吸收光谱中分离出的TAPP与DNA作用的散射成分,其形状基本一致.
Abstract:
By changing the propagation direction of the incident light beam of a common spectrofluorometer through an absorption cell holder,the incident light spectrum and the transmitted light spectrum are directly measured through a spectrofluorometer.A simple corrected resonance light-scattering technique was established to separate absorbance and scattering components from apparent absorption spectrum.With measurement of the resonance light scattering signals,after which the molecular effect was corrected,the interaction of α,β,γ,δ-Tetrakis[4-(trimethylammoniumyl) phenyl] porphine(TAPP) with DNA,the detection sensitivity could be improved about two fold and the shape of scattering components separated from F-2500 and F-4500 spectrofluorometer was identical when the instrumental effect was corrected.

参考文献/References:

[1] PASTERNACK R F, BUSTAMANTE C, COLLINGS P J. Porphyrin assemblies on DNA as studied by a resonance light scattering technique [J]. Journal of the American Chemical Society, 1993.5393-5399.doi:10.1021/ja00066a006.
[2] HUANG Chengzhi, LI Ke-an, Tong Shen-yang. Determination of nucleic acids by a resonance light-scattering technique with α, β, γ, δ-tetrakis-[4-(trimethyammomiumyl) phenyl] porphine [J]. Analytical Chemistry, 1996, (13):2259-2260.doi:10.1021/ac9511105.
[3] 杨传孝, 李原芳, 黄承志. 丽春红G用于血清总蛋白的共振光散射测定 [J]. 分析化学, 2003, (2):148-152.doi:10.3321/j.issn:0253-3820.2003.02.005.
[4] YANG Chuan-xiao, LI Yuan-fang, HUANG Cheng-zhi. Determination of proteins with fast red VR by a corrected resonance light scattering technique [J]. Analytical Sciences, 2003, (2):211-215.doi:10.2116/analsci.19.211.
[5] COLLINGS P J, GIBBS E J, STARR T E. Resonance light scattering and its application in determination the size, shape, and aggregation number for supramolecular assemblies of chromophores [J]. Journal of Physical Chemistry B, 1999.8474-8481.doi:10.1021/jp991610s.
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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20425517,20575023); 福建省自然科学基金资助项目(2006J0261); 华侨大学科研基金资助项目(06HZR15)
更新日期/Last Update: 2014-03-23