[1]方柏山,洪燕,夏启容.酶体外定向进化(Ⅱ) 文库筛选的方法及其应用[J].华侨大学学报(自然科学版),2005,26(2):113-116.[doi:10.3969/j.issn.1000-5013.2005.02.001]
　Fang Baishan,Hong Yan,Xia Qirong.Directed Evolution of Enzymes in Vitro (Ⅱ) Methods of Enzyme-Library Screening and Its Application[J].Journal of Huaqiao University(Natural Science),2005,26(2):113-116.[doi:10.3969/j.issn.1000-5013.2005.02.001]
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酶体外定向进化(Ⅱ) 文库筛选的方法及其应用()
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《华侨大学学报（自然科学版）》[ISSN:1000-5013/CN:35-1079/N]

卷:
第26卷
期数:
2005年第2期
页码:
113-116
栏目:
出版日期:
2005-04-20

文章信息/Info

Title:
Directed Evolution of Enzymes in Vitro (Ⅱ) Methods of Enzyme-Library Screening and Its Application
文章编号:
1000-5013(2005)02-0113-04
作者:
方柏山; 洪燕; 夏启容
华侨大学材料科学与工程学院; 华侨大学材料科学与工程学院 福建泉州362021; 福建泉州362021; 福建泉州362021
Author(s):
Fang Baishan;  Hong Yan;  Xia Qirong
College of Material Science and Engineering, Huaqiao University, 362021, Quanzhou, China
关键词:
酶;  筛选;  定向进化
Keywords:
enzyme;  screening;  directed evolution or orthogenesis
分类号:
Q785
DOI:
10.3969/j.issn.1000-5013.2005.02.001
文献标志码:
A
摘要:
定向进化是改造蛋白质分子的一种有效的新策略,但其构建的文库非常大,待筛选的克隆常常多达105~1010.同时在目的酶筛选中,至关重要的是找到高灵敏度、高选择性和高通量的筛选方法.居于此,文中综合评述在突变株分离、酶检测和信号探测等方面,目前的一些常用技术及其最新进展.
Abstract:
Directed evolution is an effective new strategy for remoulding molecules of protein, but a very large library is required to be constructed and the clones so much as 105~10~10 are required to be screened. Moreover, it is vital for screening object enzymes to find the method of screening with high sensitivity, high selectivity and high throughput. In view of these, the authors give here a comprehensive commentary on some common techniques in mutant isolation, enzyme assay and signal detection and their progress recently.

参考文献/References:

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[1]方柏山,郑媛媛.酶体外定向进化(Ⅰ) 突变基因文库构建技术及其新进展[J].华侨大学学报(自然科学版),2004,25(4):337.[doi:10.3969/j.issn.1000-5013.2004.04.001]
　Fang Baishan,Zheng Yuanyuan.Directional Evolution of Enzymes in Vitro (Ⅰ) Technique for Generating Mutator Libraries and Its New Progress[J].Journal of Huaqiao University(Natural Science),2004,25(2):337.[doi:10.3969/j.issn.1000-5013.2004.04.001]
[2]邓轶韬,李夏兰,陈宗香,等.阿魏酸酯酶产生菌的筛选及产酶条件的优化[J].华侨大学学报(自然科学版),2011,32(3):300.[doi:10.11830/ISSN.1000-5013.2011.03.0300]
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备注/Memo

备注/Memo:
国家自然科学基金资助项目(20276026,20446004); 福建省科技计划重点课题基金资助项目(2003I020)
更新日期/Last Update: 2014-03-23